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International
Biotechnology and Research Conference

April 25-27, 2018 | Rome, Italy

Program Schedule

  • Sessions:
    Industrial and Microbial Biotechnology

    Time:

    Title: Potential of indigenous lactococci as starter cultures in dairy industry

    Mirjana Bojanic Rasovic
    University of Montenegro, Montenegro.

    Biography
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    Biography

    Mirjana Bojanic Rasovic
    University of Montenegro, Montenegro.

    Dr. Mirjana Bojanic Rasovic began to work in 1994 at Biotechnical Institute, now Biotecnical faculty in Podgorica. From 2012, she is a senior research associate in the field of Animal hygiene and Preventive Diseases and Microbiology. For two terms (2003 to 2007), she was president of the Society of Microbiologists of Montenegro for which time she organized several scientific meetings and lectures. She has participated in the work of a number of domestic and foreign scientific and professional conferences. As author or co-author, about 80 scientific and professional papers have been published. She was the head of national projects "Influence of zoohygienic conditions on the appearance of mastitis in high dairy cows", 2001-2003., "Examination of the spread of bovine infections with Mycoplasma bovis in the Montenegro, “Isolation and characterization of indigenous lactic acid bacteria for the production of specific cheeses in Montenegro", (2009-2012). Dr Mirjana Bojanic Rasovic is Member of following committees: The Veterinary Chamber of Montenegro, Commission for Accreditation of the Accreditation Body of Montenegro, Technical Committee for Laboratories of the Accreditation Body of Montenegro, Sectoral Commission for Agriculture, Food Processing, Committee for Agriculture and Forestry of the Montenegrin Academy of Science and Arts, Commission of the Ministry of Agriculture and Rural Development for professional exam for graduate veterinarians.



    Abstract
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    Abstract

    Mirjana Bojanic Rasovic
    University of Montenegro, Montenegro.

    The diversity of lactic acid bacteria in traditional dairy products represents great potential in biotechnology. In order to standardize indigenous products, the basic requirement is the application of the determined indigenous lactic acid bacteria as starter cultures affecting their specific characteristics by performing fermentation and influencing the ripening process. Starter microorganisms produce lactic acid, which is very important during the coagulation and texturizing of the curd cheese. Production of volatile compounds (e.g. diacetyl and acetaldehyde) contribute to the flavor of these dairy products. Starter culture may posses a proteolytic and lipolytic activity that may be desirable, especially during the maturation of some types of cheese. They also produce bacteriocins, which prevents the growth of pathogens and many spoilage microorganisms. In the process od fermentation of cheese usually participate bacteria of the genus Lactococcus – Lc. lactis ssp. lactis, Lc. lactis ssp. cremoris and homofermentative lactobacilli. Lactococcus lactis species is one of the most important of lactic acid bacteria that are used in the dairy industry. The major functions of this species in dairy fermentation are the production of lactic acid from lactose, hydrolysis of casein and citric acid fermentation. Their metabolic end products and enzymes directly or indirectly have significant influence in determining the texture and flavour of the final products. The utilization of lactococci isolated from indigenous fermented milk products would lead to potential starter cultures with the necessary properties for typical local products that are well accepted by the local population. Also, the use of such starter cultures would allow the production of cheese with designated geographical origin.

    Time:

    Title: Immobilization of ARM lipase for industrial use

    Raja Noor Zaliha Raja Abd Rahman
    University Putra Malaysia, Malaysia

    Biography
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    Biography

    Raja Noor Zaliha Raja Abd Rahman
    University Putra Malaysia, Malaysia

    Raja Noor Zaliha Raja Abd. Rahman obtained her B.Sc. (Hons)-Microbiology from Universiti Sains Malaysia in 1989 and her M.S. in Microbiology from Universiti Putra Malaysia in 1994. She obtained her Doctor of Engineering in Molecular Biology from Kyoto University, Kyoto, Japan in 1998. She is currently the Professor and Head of Enzyme and Microbial Technology Research Centre, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia. In recognition of her scientific findings, she has been awarded patents, prizes and commendations and has derived personal satisfaction from the success of her graduate students, her participation on national and international governmental projects and not least, having a bacterial species named after her.



    Abstract
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    Abstract

    Raja Noor Zaliha Raja Abd Rahman
    University Putra Malaysia, Malaysia

    ARM lipase was isolated from Geobacillus sp. strain ARM, holds many potentials for industrial applications as it is thermostable, organic solvent tolerant, 1,3-regioselective. It prefers medium and long chain fatty acids as substrate. In this study, the enzyme was overexpressed in Escherichia coli system and then purified using affinity chromatography. The purified enzyme was immobilized in gelatinized sago solution and spray-dried by entrapment technique in order to enhance the enzyme operational stability for handling at high temperature and also for storage. The physical characteristic of the immobilized enzyme was studied by scanning electron microscopy, surface area and porosity. The immobilized ARM lipase showed good performance at high temperature. The immobilized enzyme could be stored at for few months without loss of activity. Collectively, the immobilized lipase shows promising capability for industrial use.

    Time:

    Title: Efficient Retting of Natural Fibre Using Microbial Enzyme

    Wan Zuhainis Saad
    Universiti Putra Malaysia, Malaysia.

    Biography
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    Biography

    Wan Zuhainis Saad
    Universiti Putra Malaysia, Malaysia.

    Wan Zuhainis Saad is an associate professor in Microbiology. Research activities include drug discoveries from thermophilic fungi and lactic acid bacteria, microbial enzymes technology in biopolymers and pulp and papers. Awarded three patents on isolation and preservation techniques of thermophilic fungi and enzyme for bioretting of kenaf. An Educational Technology enthusiast. Practices E-learning and active learning approach in enhancing students’ engagement for effective and meaningful learning. Shortlisted for the QS Wharton Reimagine Education Award 2015 and 2016. QS Wharton Reimagine Education Awards Judge 2017. Awarded Vice-Chancellor Fellowship Award (Young Educator) 2012 and Vice-Chancellor Fellowship Award (Excellent Educator) 2016.



    Abstract
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    Abstract

    Wan Zuhainis Saad
    Universiti Putra Malaysia, Malaysia.

    The global natural fibre composites market is forecast to grow 8.2% from 2015 to 2020. The major driver for the growth of this market is; The rise in demand for lightweight and environmentally sustainable composite materials in various applications, such as automotive, building & construction, and others.In this market, natural fibre (hemp, flax, jute, kenaf, and others) are the major raw materials used for producing natural fibre composites. Kenaf also known as Hibiscus cannabinus is an annual fibre plant closely related to cotton. The kenaf bast fibre found its application in many industries such as textile industry, automotive industries, structural and building materials and made into biocomposite consumer products. The process of separating the bast from the core by degrading the pectin rich middle lamella is known as retting. The traditional method of water retting requires a longer retting time and caused pollution while dew retting produced fibres with poor quality. Hence, there is a need to seek for an environmental-friendly approach to produce high-quality kenaf bastfibres. In this study, pectinolytic fungi were isolated from various sources and screened for their pectinase activity. A potential pectinase producing strain was chosen and identified as Aspergillus fumigatus R6 by amplification of the Internal Transcribed Spacer region. It was found the retting process using the enzyme has reduced the retting time from 3 weeks to 3 days. Kenaf bast fibres quality can improve by further optimisation of the enzyme formulation. Aspergillus fumigatus R6 pectinase enzyme showspotential to be used in kenaf bast bio retting process to produce strong and high quality kenaf long bast fibres.

    Time:

    Title: Production and Evaluation of Protease Enzyme (B.subtilis strain) for Eco-friendly Leather Processing Empowerment in Ethiopian Tanneries

    Biruk Abate
    Ku Lueven Engineering College, Belgium.

    Biography
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    Biography

    Biruk Abate
    Ku Lueven Engineering College, Belgium.



    Abstract
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    Abstract

    Biruk Abate
    Ku Lueven Engineering College, Belgium.

    Production and Evaluation of Protease Enzyme (B.subtilis strain) for Eco-friendly Leather Processing Empowerment in Ethiopian Tanneries

    Time:

    Title: Sustainable and Efficient Recovery of Polyhydroxyalkanoate Polymer from Cupriavidus necator using Environment Friendly Solvents

    Geeta Gahlawat
    Panjab University, India

    Biography
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    Biography

    Geeta Gahlawat
    Panjab University, India

    Dr. Geeta Gahlawat is presently working as UGC-Kothari Postdoctoral fellow at Panjab University, India. She obtained his Ph.D. degree in November 2014 from Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, India. During PhD, she has worked on the project entitled "Microbial production of Polyhydroxybutyrate (PHB) and its copolymers". She has more than 8 years of experience in fermentation and microbial technology along with bioreactor operation and their maintenance. She has systematically built a firm foundation of bioprocess engineering fundamentals to optimize any fermentation processes. She has published eight papers in peer reviewed International Journals.



    Abstract
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    Abstract

    Geeta Gahlawat
    Panjab University, India

    An imprudent use of environmentally hazardous petrochemical-based plastics and limited availability of fossil fuels have provoked research interests towards production of biodegradable plastics - polyhydroxyalkanoate (PHAs). However, the industrial application of PHAs based products is primarily restricted by their high cost of recovery and extraction protocols. Moreover, solvents used for the extraction and purification are toxic and volatile which causes adverse environmental hazards. Development of efficient downstream recovery strategies along with utilization of non-toxic solvents will accelerate their commercialization. In this study, various extraction strategies were designed for sustainable and cost-effective recovery of PHAs from Cupriavidus necator using non-toxic environment friendly solvents viz. 1,2-propylene carbonate, ethyl acetate, isoamyl alcohol, butyl acetate. The effect of incubation time i.e. 10, 30 and 50 min and temperature i.e. 60, 80, 100, 120°C was tested to identify the most suitable solvent. PHAs extraction using a recyclable solvent, 1,2 propylene carbonate, showed the highest recovery yield (90%) and purity (93%) at 120°C and 30 min incubation. Ethyl acetate showed the better capacity to recover PHAs from cells than butyl acetate. Extraction with ethyl acetate exhibited high recovery yield and purity of 96% and 92%, respectively at 100°C. Effect of non-toxic surfactant such as linear alkylbenzene sulfonic acid (LAS) was also studied at 40, 60 and 80°C, and detergent pH range of 3.0, 5.0, 7.0 and 9.0 for the extraction of PHAs from the cells. LAS gave highest yield of 86% and purity of 88% at temperature 80°C and 5.0 pH.

    Time:

    Title: Purification of α-amylase from thermophilic Bacillus licheniformis and its application in the production of ethanol

    Reena Gupta
    Himachal Pradesh University, India

    Biography
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    Biography

    Reena Gupta
    Himachal Pradesh University, India

    Presently working as Professor in the Department of Biotechnology, Himachal Pradesh University, Summerhill, Shimla. Area of specialization is Biochemistry, Immunology and Enzyme Technology. Completed B.Sc. (Hons.) and M.Sc. (Hons.) in Biochemistry from Panjab University, Chandigarh in the year 1985 and 1987 respectively. Ph.D. was completed in the year 1992 from Panjab Unversity, Chandigarh and Post Doctorate from May 1993 to Oct. 1995 from IMTECH (Institute of Microbial Technology), Chandigarh. She has a 22 years of teaching and 29 years of research experience. She completed four Research Projects funded by UGC, New Delhi and one Project funded by DEST, Shimla is ongoing. The amount of total fund generated through Projects is 20.54 lakhs. Total number of publications in peer reviewed Journals of international repute are 87 (National and International). She is a Member of five National Professional Bodies.



    Abstract
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    Abstract

    Reena Gupta
    Himachal Pradesh University, India

    An a-amylase from thermophile Bacillus licheniformis was purified by using DEAE- Cellulose column chromatography followed by Sephadex G-75 column chromatography. Native and SDS-PAGE analyses showed a single band for the purified enzyme, with an apparent molecular weight of 33 kDa. This showed that the enzyme was purified to homogeneity, was a monomer and had no subunits. The α-amylase immobilized on WhatmanTM filter paper showed maximum activity at 55°C of incubation temperature, 10 min of incubation time and pH 8.5 of 0.1M Tris-HCl buffer. The immobilized enzyme gave maximum activity with 0.175% (w/v) of substrate (starch) concentration and showed Km and Vmax value of 0.74 mM and 6.39 μmol/g/min respectively. Immobilized enzyme retained almost 50.25% of its original activity after 9th cycle of reusability. During storage, immobilized enzyme retained relative activity of 90.87% and 56.28% upto 10 and 40 days respectively. The immobilized enzyme produced maximum amount of reducing sugar from maize stalk juice extract when incubated at 55°C of temperature at pH 8.5 and for 20 min of incubation. It was observed that there was a 50-fold increase in production of ethanol from extract of maize stalk treated with immobilized α-amylase (27.33%) as compared to the untreated sample (18.92%).

    Time:

    Title: Enhanced Production, Purification and Thermodynamic Characterization of Endotoxin Free Antileukemic Recombinant L-Asparaginase of Escherichia Coli K-12

    Santosh Kumar Jha
    BITS, Indai.

    Biography
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    Biography

    Santosh Kumar Jha
    BITS, Indai.

    L-Asparaginase (E.C. 3.5.1.1) is a well accepted chemotherapeutic agent against the acute lymphoblastic leukemia and lymphosarcoma. The recombinant L-asparaginase enzyme was produced by the over-expression of ansB gene of E. coli K-12 in E. coli BL21. Different carbon sources, nitrogen sources, minerals and additives having yield enhancing effect, were screened by Plackett Burman Design (PBD). Their optimum level was identified by the using the orthogonal array method of Taguchi design of experiment. The screening of media components by PBD proposed the glucose as main carbon source, tryptone and yeast extract as organic nitrogen source, NH4Cl as inorganic nitrogen source, NaCl and K¬2HPO4 as mineral source have significant effect on the production of enzyme. Glycerol was identified as the most influential effect on the recombinant L-asparaginase production among the all additives. The addition of small amount (0.6% v/v) of it, significantly increased the enzyme yield. After the complete optimization of the selected process parameters 121.8% enhanced production of L-asparaginase was observed at shake flask level. There was further 14.8% enhancement in the enzyme production after the scale up the process in 5L bioreactor. The volumetric yield of 3.58 X 105 U/L of L-asparaginase with the specific activity of 6.97 X103 U/mg in fermentation broth was reported. The enzyme was purified by affinity chromatography followed by three-phase partitioning. The endotoxin level was estimated by LAL-assay. The purified recombinant enzyme was further used to study the thermodynamic parameters. The enzyme showed highest stability at 280C than at higher temperatures with a half-life of 46 hrs which is quite significant. Its deactivation energy was found to be 60.64 kJ/mol. The value of thermodynamic parameters including ∆H, ∆S and ∆G were found to be -49.23kJ/mol, 0.09kJ/mol.K and 73.12-74.78 kJ/mol respectively implying that there are no significant processes of aggregation and the enzymatic reaction was exothermic and spontaneous in nature. The antileukemic potential of the enzyme was accessed by using human leukaemia cell line HL-60 through viability study, cell and morphological studies.



    Abstract
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    Abstract

    Santosh Kumar Jha
    BITS, Indai.

    Dr. Santosh Kumar Jha is an academician cum researcher and presently serving as an Assistant Professor in the Birla Institute of Technology, Mesra, India. He has expertise in the process development and designing of bioprocess for the production of biotherapeutics. Presently he is working on the bioprocess development of various biotherapeutics, development of nanobiocomposite based nanobiotics and tissues scaffolds for various biomedical applications.

    Sessions:
    Biosensors and Biomarkers

    Time:

    Title: QCM-basedbiosensors for the detection of tumorreleasedexosomes

    Agnese Magnani
    University of Siena, Italy

    Biography
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    Biography

    Agnese Magnani
    University of Siena, Italy

    Agnese Magnani is a Professor of Inorganic Chemistry- University of Siena – Department of Biotechnology, Chemistry and Pharmacy. Her research topics are: - Functional polymers, materials and coatings for biomedical and agriculture applications - Biosensors for biomarkersidentification and determination in body fluids - Nanocarriers for drug target delivery - Study of molecular recognition processes: protein-ligand interactions; protein and cell interaction with solid surfaces; biofilm formation - Application of IR and ToF-SIMS to materials and biological systems: thin films, SAMs and nanomaterialscharacterisation; geographical characterisation of agrifood products; development of micro-imaging methods for biological processes in tissues and cells.



    Abstract
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    Abstract

    Agnese Magnani
    University of Siena, Italy

    Biosensors cansatisfy the rapidityand accuracydiagnosisrequirements in cancerbiomarkers(tumorassociatedantigens) detectionduringearlystages of the disease, thusovercomingmany of the problemsrelated to the classicaldiagnosticmethods more expensive and often time consuming. Modernbioaffinitysensors, suchas DNA- or immunosensors, haverecentlydemonstratedgreatpotential for monitoringcancer-relatedproteinmarkers and DNA mutations. Mass-sensitive devices like Quartz Crystal Microbalances (QCMs) are commonly used to develop bio-affinity sensors: this kind of sensor is usually made of an AT-cut quartz disk with electrodes on both sides, one of which is functionalized with a receptor selective to the target analyte. If the quartz is used as a feedback element in an electronic sinusoidal oscillator (setting in this way the oscillation frequency), in case of a bio-recognition event, the change of the mass of the quartz is proportionally converted to a frequency shift of the oscillator frequency, providing an indirect measure of the adsorbed mass with a good sensitivity (1Hz/ng for 10 MHz AT-cut quartzes). The development and the characterization of QCM biosensors for the detection of exosomes is presented. Exosomes are cell-derived vesicles that are present in many biological fluids, and that possess diagnostic potential in the oncologic field. From tests with physiological solutions and human plasma, the developed biosensors have proved to give a rapid response (within minutes) with high sensitivity and specificity against the PSMA (prostate-specific membrane antigen).

    Sessions:
    Biotechnology in Agriculture

    Time:

    Title: Plant Regeneration from Immature Embryos of Foxtail Millet: A Millet and Biofuel Crop

    Kokiladevi Eswaran
    Agriculture College Research Institute, India.

    Biography
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    Biography

    Kokiladevi Eswaran
    Agriculture College Research Institute, India.



    Abstract
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    Abstract

    Kokiladevi Eswaran
    Agriculture College Research Institute, India.

    The small millets comprising six species, namely, finger millet (Eleusinecoracana), little millet (Panicumsumatrense), Italian or foxtail millet (Setariaitalica), barnyard millet (Echinochloacrusgalli), proso millet (Panicummiliaceum) and kodo millet (Paspalumscrobiculatum) are grown in about 2 million ha area in India, Among these, finger millet is the most important and occupies about 60% of the area and contributes 70 % of small millet production. These crops are hardy and quite resilient to varied agroclimatic adversities and play important role in marginal agriculture more common in hilly and semi-arid regions as important source of food grain as well as highly valued fodder. Many kinds of traditional foods and beverages are made from these grains in different regions and hence have important role in the local food culture. Nutritionally, they have high micronutrient content, particularly calcium and iron, high dietary fibre, higher amount of essential amino acids and low glycemic index and thus play an important role in the food and nutritional security of the poor. However, their presence in the Indian food basket had been declining over the years primarily due to wheat and rice being available at subsidized rates. These species are neglected in research and development and are not receiving the policy support they need and rightly deserve. This neglect is also causing the marginalization of farmers who have been traditionally depending on these crops for their food security and income. However, there is an increasing recognition of their favourable nutrient composition and utility as health food, in the context of increasing life style diseases. Thus, apart from their traditional role as a staple for the poor in the marginal agricultural regions, they are gaining a new role as crops for healthy food and for the urban high income people. To meet the strong increase in cereal demand worldwide, new approaches and technologies for generating new varieties are necessary. One of these methods is the creation of transgenic plants with desirable traits. Although millets are economically important, especially in the developing world, little genetic improvement has been done so far specifically using wide- or cross- hybridization among closely related species. The incompatibilities due to interspecific hybridization are alleviated by directly transferring the desirable traits to millets using optimum or efficient transformation method. Hence, crossing barriers could be overcome, and genes from unrelated sources would be introduced asexually into crop plants. Monocots in general and cereals in specific were initially difficult to genetically engineer, mainly due to their recalcitrance to in vitro regeneration and their resistance to Agrobacterium-mediated infection. However, efficient transformation protocols have been later established for the major cereals including rice and maize. Gene transfer to millets would be facilitated once efficient or optimum regeneration and transformation techniques are established. The optimization of regeneration method is, therefore, necessary for different millet types in order to increase the efficiency of transformation. Hence, a study was taken in foxtail millet to develop tissue culture regeneration protocol for high frequency shoot regeneration from immature embryos through somatic embryogenesis. In this study,we report optimization of the regeneration system for foxtail millet through improvement of the regeneration system efficiency. Immature inflorescences explants of foxtail millet cv. Co (Te)7 varying in length (0.5 to 1.0 cm) were cultured on modified MS medium for callus induction and regeneration. Calli were obtained at the highest frequency (80%) from immature inflorescences on MS containing 2, 4 D (2.5mg/L). The efficiency declined dramatically namely 40, 60 and 60% in MS medium containing 2, 4, D 1.0, 2.0 & 3.0 mg/L. The results indicate that 2, 4, D (2.5mg/L) is the best concentration for callus induction. To assess the regeneration potential, calli induced from MS medium containing 2, 4, D (2.5mg/L) were transferred to regeneration medium with different concentrations of BAP. Differentiation was defined as the development of green spots distributed discretely throughout the callus within two weeks of transfer. The differentiation rate (percentage of differentiated calli among the total number of embryogeniccalli) was varying. The calli induced from 2,4 D (2.0 mg/L) and cultured for 25 days in regeneration media containing BAP (1.5 mg/L) showed the highest differentiation frequency. The differentiation frequency decreased with increasing concentration of BAP.Shoots were developed from the green spots after one-month culture on regeneration medium and the number of shoots per callus varied from one to ten. Shortly after, the shoots (about 1 cm long) were excised and transferred to rooting medium (1/2MS + 1 mg/l L-Proline + 800 mg/l Casein Hydrolysate + 3 % (w/v) Sucrose + 0.3 % (w/v) Phytagel, pH 5.8) for two weeks until roots developed. Regenerated plantlets with fully grown shoots and roots were gradually acclimatized in the greenhouse and the survival rate was more than 90%. These results demonstrate that calli induced from 0.5 to 1.0 cm immature inflorescences in MS containing 2, 4 D (2.5 mg/L) is optimal for regeneration. The regeneration frequency is more in MS media containing BAP (1.5 mg/L).Regenerated plantlets were further rooted on the medium in bottles. Thereafter, rooted plants were grown to maturity in the greenhouse and allowed to set seeds.

    Time:

    Title: An Enzyme To Increase Seed Vigor & Longevity

    Zhanar Chunetova
    Al-Farabi Kazakh National University, Kazikisthan.

    Biography
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    Biography

    Zhanar Chunetova
    Al-Farabi Kazakh National University, Kazikisthan.



    Abstract
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    Abstract

    Zhanar Chunetova
    Al-Farabi Kazakh National University, Kazikisthan.

    Mutagenic effect of physical factors and chemical substances (aziridine or ethylene imine, nitrosoethylurea, nitrosoethyleneurea) leads to increase of the spectrum of hereditary variability for breeding purposes, which however is not studied in full extent. Ecological study of anthropogenic factors action leading to disruption of certain links between chemical elements and their combinations, raise of heavy metals concentration in soil, facilitate examination of mutagenic and toxic properties of heavy metals. Increase in wheat yields by improving its genotype is one of the most urgent problems of agriculture and economy. Atpresent, using traditional methods of selection and genetic studies, such as backcross selection, distant hybridization, and experimental mutagenesis, increased efficiency of obtaining genetically modified and improved forms of wheat [1-5]. Heavy metals are defined as metals having a density higher than 5 g/cm3. Of the total 90 naturally occurring elements divided into three classes by the degree of their threat, 53 are considered heavy metals and few are of biological importance. Accumulation of heavy metals such as cadmium (Cd) in the environment is now becoming a major cause of environmental pollution. Toxic metals can inactivate proteins, shifting metal cofactors, blocking active centers or causing allosteric changes. Besides, large number of those possesses ability of inducing mutagenic changes, tumors and causing macroscopic changes. Molecular mechanism of heavy metals toxicity is not completely understood. Cd is non-essential element that negatively affects plant growth and development, released into the environment by power stations, heating systems, metal working industries or urban traffic, which has high cumulative effect with almost no biodegradation. In plants it affects such processes as stomata opening, transpiration and photosynthesis, consequently chlorosis, leaf rolls and stunting are the main symptoms of Cd toxicity in plants accompanied by root browning, leaf red-brownish discoloration. It can also reduce the absorption of nitrate from root to shoot by inhibiting the nitrate reductase activity in shoots. The negative effect of Cd on plant growth was accompanied by an increase in dry to fresh mass ratio in all organs. Several researches have suggested that an oxidative stress could be involved in cadmium toxicity, by either inducing oxygen free radical production, or by decreasing enzymatic and non-enzymatic antioxidants [6-9]. On the other hand, the use of induced mutagenesis showed high efficiency in the production of forms with high yield, quality bakery, lodging resistance, modified plant height and resistance. And, this paper is an attempt of summarizing results performed by our group in this direction.

    Time:

    Title: Protein L-Isoaspartyl Methyltransferase

    Manoj Majee
    National institute Of Plant Genome Research, India.

    Biography
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    Biography

    Manoj Majee
    National institute Of Plant Genome Research, India.



    Abstract
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    Abstract

    Manoj Majee
    National institute Of Plant Genome Research, India.

    Seed longevity is a vital trait for the germplasam conservation of endangered and cultivated species. Maintenance of seed vigor and longevity during storage is also one of the important concerns in Agriculture, since under sub tropical climate, seeds of most crop species show rapid deterioration and exhibit reduced seed longevity. Recently in our lab, we demonstrated the role of Protein L-Isoaspartyl Methyl Transferase (PIMT) in enhancing seed vigor and longevity. PIMT is a protein repairing enzyme and catalyzes the conversion of spontaneously modified isoAsp to Asp in protein. Our study in rice (Oryza sativa) demonstrated that PIMT activity sharply increases at maturation phase, is retained in dry seed and then declines upon completion of germination. Likewise, deleterious isoaspartyl accumulation also increases during seed maturation and is highly abundant in dry seed but decreases upon imbibition. Rice possesses two genes (OsPIMT1 and OsPIMT2) which encode multiple PIMT isoforms. Transcript and western blot analyses clearly demonstrated distinct tissue and seed development stage specific accumulation of these PIMT isoforms, indicating their participation and specific contribution in seed desiccation, longevity and germination in rice. Further analyses of overexpression transgenic lines for each OsPIMT isoform revealed that they play a distinct role in restricting deleterious isoAsp and ROS accumulation to improve seed vigor and longevity. Overall, our study demonstrated that PIMT enzyme can be exploited to maintain high seed vigor ensuring a vigorous crop establishment in wide environmental conditions and would be substantial benefit to society

  • Keynote Speaker

    Time:

    Title

    Title: Tumor Extracellular Matrix Microenvironment, Role of Macrophages

    Henry Lopez
    Murigenics, USA.
    Biography
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    Biography

    Henry Lopez is a founder, CEO/CSO of MuriGenics, Inc. which is a preclinical contract research organization working with national and international companies, located in Vallejo, California. Prior to this he worked with many Northern California biotechnology companies such as Pfizer, Parke-Davis, GeneNetworks, Glycomed, Xoma and Cetus. In 2012 he founded Riptide Bioscience (Senior Vice President) a synthetic peptide company focusing on immunomodulation and antimicrobials. Have licensed out one peptide to Pharma for treating pancreatic cancer- working partner has been the NCI for the last 4 years. He received PhD from the University College London –Royal Free Hospital.



    Abstract
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    Abstract


    Sessions:
    Biotechnology and Applied Biochemistry

    Time:

    Title: Molecular insights into membrane trafficking by the SNX27-retromer complex

    Mintu Chandra
    The University of Queensland, Australia.

    Biography
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    Biography

    Mintu Chandra
    The University of Queensland, Australia.



    Abstract
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    Abstract

    Mintu Chandra
    The University of Queensland, Australia.

    Compartmentalisation is a defining feature of all eukaryotic cells, and we have evolvedhighly sophisticated protein machineries to control the flow of transmembrane moleculesand membrane lipids between different organelles. Disruption of these processes are linkedto numerous diseases including neurodegenerative disorders, pathogen invasion andcancer. We are determining how these trafficking machineries are assembled and regulatedat the molecular level through a combination of structural biology, biophysical, and cellbiology approaches. In my talk, I will describe our most recent work on critical protein sortingmachineries – the retromer complex and the sorting nexins (SNXs) - regulating endosomalmembrane recycling and cellular homeostasis. We have defined sorting signals requiredfor endosomal recycling by the SNX27-retromer complex, how this is regulated by posttranslationalphosphorylation, and the structural basis for SNX27-retromer-cargoassembly.

    Time:

    Title: New tool for enzymatic analysis in raw materials

    Julia Schuckel
    Glycospot, Denmark.

    Biography
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    Biography

    Julia Schuckel
    Glycospot, Denmark.

    Dr. Julia Schückel holds a M.Sc. degree in chemistry from the Technical University of Dresden, Germany and a PhD degree in biochemistry from the University of York, United Kingdom. She developed together with her colleague Stjepan K. Kracun the screening technology for testing enzyme activities in raw material.



    Abstract
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    Abstract

    Julia Schuckel
    Glycospot, Denmark.

    Industries, such as pharma, feed, and food, are increasingly looking for enzymes to solve production and product issues. Correct and adequate information on enzyme activity would help producers avoid prolonged production times, reduce waste, improve end-products, prolong shelf-life and save costs for overtreatment. Most of the production processes turning raw materials into products are under the influence of a wide variety of enzyme combinations. Existing tools for screening of enzymatic activity, are cumbersome, time-consuming and often requires expert staff. Each assay needs to be constructed from the bottom up forcing the user to aliquot substrates with high precision into a large number of reaction vessels manually. Furthermore, all existing assays measure the activity of one enzyme only at a time. We have developed a ready-to-use screening technology, where it is possible to detect important enzymatic activity in raw material. We will show different examples of endogenous enzymatic activity in the grain, flour and malt. This data helps to minimize uncertainties when using technical aids and eventually, desired properties of the final product are ensured.

    Sessions:
    Biotechnology for Genetic disorders

    Time:

    Title: Genomic Modification By Ocimum canum Against Lead-Induced Chromosom -Aberration And Its Effect On Antioxidant Enzymes

    Oladimeji Tugbobo
    Federal Polytechnic, Nigeria.

    Biography
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    Biography

    Oladimeji Tugbobo
    Federal Polytechnic, Nigeria.



    Abstract
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    Abstract

    Oladimeji Tugbobo
    Federal Polytechnic, Nigeria.

    Anticlastogenic potential of Ocimum canum (Black leaf) extract was studied in bone marrow cells of mice using micronucleus assay. 200mg/kg of Ocimum canum aqueous extract was administered as dietary supplement for 30-days. The mice were divided into three groups A, B and C. Animals in group A were fed with distilled water, B were treated with 2.5mg/kg lead acetate while group C were fed with 200mg/kg Ocimum canum aqueous extract and 2.5mg/kg lead acetate simultaneously. After 30-days, mice were sacrificed and chromosome preparations were made from bone marrow according to colchicines hypotonic-fixation air drying Giemsa schedule. The cytogenic end-point observed was chromosomal aberration which increased significantly (P<0.05) in group B animals treated with lead acetate only. However, the chromosomal aberration was significantly (P<0.05) reduced by the extract fed to animals in group C. In addition, the effect of the extract on the defensive antioxidant enzymes of the test animals was also assessed. The results indicate synergistic effect of the extract on the antioxidant enzymes in the liver tissues. Hence, the results of this study suggest viable anticlastogenic and antioxidant potentials of Ocimum canum extract which could protect against lead-induced chromosomal aberration and as well enhance activities of antioxidant enzymes.

    Sessions:
    Biotechnology Applications in Genomics and Proteomics

    Time:

    Title: CRISPR/CAS9 TARGETING Micro RNA-24 IN Chinese Hamster Ovary Cells increases growth and boosts productivity

    Kevin Kellner
    NICB, Ireland.

    Biography
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    Biography

    Kevin Kellner
    NICB, Ireland.



    Abstract
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    Abstract

    Kevin Kellner
    NICB, Ireland.

    Chinese Hamster Ovary (CHO) cells are the prominent cell line used in biopharmaceutical production. Although optimisation efforts have led to a vast increase in productivity, CHO cells yield less than other expression systems like yeast or bacteria. To improve yields and find beneficial bioprocess phenotypes, genetic engineering plays an essential role in recent research. The miR-23 cluster with its genomic paralogues (miR-23a and miR-23b) was first identified as differentially expressed during temperature shift, suggesting its role in proliferation and productivity. The common approach to deplete miRNAs is the use of a sponge decoy which, requires the introduction of reporter genes. As an alternative this work aims to knockdown miRNA expression using the recently developed CRISPR/Cas9 system which does not require a reporter transcript. This system consists of two main components: the single guide RNA (sgRNA) and an endonuclease (Cas9) which induces double strand breaks (DSBs). These DSBs can result in insertion or deletion (indels) of base pairs which can disrupt miRNA function and processing. A CHO-K1 cell line stably expressing an IgG was used for knockdown experiments. SgRNAs were designed to target the seed region of miR-24-3p and stable mixed populations were generated. It was shownthat miRNA expression for miR-24-3p as well as miR-24-5p was significantly reduced in mixed populations. Furthermore, an increase in pre-miR-24 was exhibited suggesting impaired processing by Drosha or Dicer. A knockdown up to 95% was achieved for miR-24 as well as the passenger miRNA (Figure 1A, B and C). The other members of the cluster which are located proximal to miR-24 were not affected by the knockdown (Figure 1D). Depletion of miR-24 showed increased proliferation in batch culture as well as boosted productivity (Figure 1E and F). However, faster growth led to increased nutrient demand of miR-24 depleted cultures and a reduction in culture time was exhibited. Quantitative label-free LC/MS was used to identify 81 more abundant protein targets. Pathway analysis revealed proteins potentially involved in enhanced ribosomal RNA biogenesis, recycling and assembly of ribosomal subunits. Furthermore, proteins involved in catalysing the loading of cognate aminoacyl-tRNAs and release of deacetylated tRNAs were higher expressed. These targets were highlighted as potential cell line engineering targets to improve productivity of CHO cells. Out of all 81 upregulated proteins, 50 were predicted targets of either miR-24-3p or miR-24-5p. In this work, we have shown that CRISPR/Cas9 can be successfully applied as a tool to knockdown miRNA expression in CHO cells. The data was generated using mixed pools and it remains to be established if both alleles can be successfully targeted e.g. using next-generation sequencing ofindividual clones.

    Sessions:
    Cell and Molecular Biology

    Time:

    Title: Manipulation of tRNA Genes to Enhance Cellular Productivity of Biologics

    Bob White
    York University, England.

    Biography
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    Biography

    Bob White
    York University, England.

    Bob White studied BiochemistryatUniversity of Oxford and Molecular Biology at the National Institute for Medical Research. He then spent 5 years at University of Cambridge before establishing his own laboratory at University of Glasgow. In 2013 he became Chair of Biochemistryat University of York. His research focuses on gene expression and has been published in Nature, Science and Cell (>6400 citations, h-index 44). He has received several national and international awards and is a Fellow of The Royal Society of Edinburgh, The Academy of Medical Sciences and The European Academy of Cancer Sciences.



    Abstract
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    Abstract

    Bob White
    York University, England.

    Recombinant monoclonal antibodies are a powerful class of therapeutics that are used to treat a wide range of diseases, including cancers and inflammatory disorders. However, they are expensive to produce and this restricts their availability to patients; health authorities sometimes cannot afford the costs of these therapies. Lowering production costs may increase access to potentially life-saving treatments. Through synthetic cell bioengineering, we are developing novel approaches to improve production of therapeutic proteins. One of our strategies is to manipulate tRNA expression to enhance translation of recombinant products. Our progress in this regard will be described. We aim to reduce costs and thereby increase availability to patients of therapeutics that at present are prohibitively expensive.

    Sessions:
    Environmental Biotechnology and Biodiversity, Waste water treatment

    Time:

    Title: Assessing the microbiome dynamics in three photo-bioreactors established for coking wastewater treatment: An orchestration between microalgae and bacterial communities

    Mariam Hassan
    Cairo University, Ezypt.

    Biography
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    Biography

    Mariam Hassan
    Cairo University, Ezypt.

    Mariam Hassan is an Assistant Lecturer in Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University. She teach the practical courses to the undergraduate and graduate students. She is working on my PhD project on biomonitoring water microbiome(s) involved in biological wastewater treatment processes. She had a experience on experience: microbiology, biotechnology, biodiversity, bioinformatics and high through put data analysis.



    Abstract
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    Abstract

    Mariam Hassan
    Cairo University, Ezypt.

    The investigation of microbial community structures is a significant way to understand biodegradation capacities in biological wastewater treatment processes. Photo-bioreactors A, B and C received real coking-wastewater as influent with COD 776 ± 56, 1229 ± 85 and 2033 ± 27 mg/l, respectively. In phase-1 phenol was added to the influent, while dichlorophenol was added in combination with phenol in phase-2. Treatment efficiency of algal-bacterial systems was biomonitored using different bioassays (phytotoxicity, Artemia toxicity, cytotoxicity, algal-bacterial ratio and settleability). COD removal %, phenol and dichlorophenol concentrations were also monitored. All systems efficiently detoxified the influents in phase-1. In phase-2, Systems B and C failed to detoxify the influents. Illumina-sequencing generated 2119749 effective sequences of 16S-rRNA gene from 21 samples collected from different influents and effluents. The number of observed species was significantly lower in effluent samples than influent samples, as some taxa dominated in photo-bioreactors and contributed to the systems performance. Significant difference in the microbial diversity between influent and effluent samples was detected. Proteobacteria (78 %), Firmicutes (12 %), Bacteroidetes (5 %) and Deferribacteres (2 %) were the dominant phyla in influent samples. While in effluent samples Proteobacteria (68 %) and Bacteroidetes (25 %) dominated. Failure in treatment process in systems B and C at phase-2 was accompanied with significant difference in the microbial diversity. Significant relative abundance of anaerobic bacteria from Deferribacteraceae and Peptococcaceae families in influent samples conformed to the nature of coking-wastewater. The co-culture of microalgae shifted the microbiome and promoted the activity of genera affiliated to Chitinophagaceae, Pseudomonadaceae and Xanthomonadaceae families, which dominated in effluent samples. These bacteria are known for their catabolic diversity that enables xenobiotic degradation. The superiority of algal-bacterial systems for coking-wastewater treatment was confirmed as co-culture of microalgae eradicated pathogenic bacteria such as Arcobacter and Legionella genera in the treated effluent.

    Time:

    Title: Phenotypic and molecular characterization and rapid evaluation of oil-degrading native bacteria isolated from different habitats in UAE

    Ismail Saadoun
    Sharjah University, UAE

    Biography
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    Biography

    Ismail Saadoun
    Sharjah University, UAE



    Abstract
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    Abstract

    Ismail Saadoun
    Sharjah University, UAE

    Despite the beneficial value of crude oil and its derivatives to the country’s economy, it could have a huge adverse impact on the environment specifically when accidently spilled to the water and soil; thus, makes it as an environmental catastrophe. This study addresses the role of identified microorganisms as hydrocarbon degraders to be used as an eco-friendly solution in oil spill bioremediation. Successfully, 19 bacterial isolates were recovered from different habitats including crude oil wells, soil treated with diesel, oil contaminated seawater and surface hydrocarbon sediment. The isolates were assessed for degradation of different hydrocarbon compounds by agar hole-plate diffusion method. Results indicated the recovery of 9 isolates namely (2A, 1D, So1, S1A, S3, KF1, SO2, AJ1 and 2B) which were identified as positive degraders for one or more of the tested hydrocarbon compounds including diesel, pentane, hexane, heptane and tetradecane. These isolates were identified biochemically using VITEK 2 microbiology system as 2A (Pseudomonas stutzeri 97%),1D (Kocuriakristinae 92%), SO1 (Staphylococcus aureus 93%), S1A (Leuconostocmesenteroides ssp. Cremoris 90%), KF1 (Rhizobium radiobacter 99%), and S3 (Staphylococcus hominis96%). Almost all of these isolates were able to utilize heptane as a sole carbon source for their survival with the isolates SO2, S1A and S3 being the most potent ones observed by their growth around the agar hole-plate. PCR analysis of the positive hydrocarbon degrading isolates for the presence of alkBgene showedtwo groups with different band size products; group 1 (G1) (~330 bp) and group 2 (G2) (multiple of 330 pb). This may imply that alkB gene can be found in multiple homologues as shown in G2 and each one may cover degradation of specific carbon number range in the tested hydrocarbon compound. Rapid evaluation of hydrocarbon compounds degradation by the native microbial communities was shown to be successful with considerable biodegradation role exploited by the recovered isolates.

    Sessions:
    Food Biotechnology

    Time:

    Title: Geographical origin of food by multivariate ToF-SIMS analysis

    Marco Consumi
    University of Siena, Italy.

    Biography
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    Biography

    Marco Consumi
    University of Siena, Italy.

    Dr. Marco Consumi is a post-doc at university of Siena. He received his Ph.D. in Biomaterials on hydrogel biomedical application and, actually his research focus on polymer based materials for controlled release of active substances in pharmaceutical and nutraceutical field. As a postdoctoral fellow, he was focused on understanding the correlation between the chemical composition of materials and their biological activity. He has also broad expertise in synthesis modification and characterization of polymers (naturals and synthetics) and materials for biomedical applications. Actually, involved in 2 EU projects on bacterial biofilm.



    Abstract
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    Abstract

    Marco Consumi
    University of Siena, Italy.

    In this work, time-of-flight secondary ion mass spectrometry (ToF-SIMS) in conjunction with Multivariate analysis (MVA) are applied to study the chemical composition and variability of different food matrices: - Sardinian myrtle (Myrtus communis L.): analysis of both berries alcoholic extracts and berries epicarp. through the. ToF-SIMS spectra of berries epicarp show that the epicuticular waxes consist mainly of carboxylic acids with chain length ranging from C20 to C30, or identical species formed from fragmentation of long-chain esters. PCA of ToF-SIMS data from myrtle berries epicarp distinguishes two groups. Seggianese olives and olive oil: three different groups of Seggianese olives: (i) treated with an insecticide (dimethoate) and a fungicide (copper oxychloride) (TU); (ii) untreated (UT); and (iii) treated-washed (TW) have been analyzed by TOF-SIMS. Intact olive slices and olive oil were analyzed. Principal component analysis (PCA) of the Tof-SIMS spectra was used to investigate similarities among samples. Peaches and nectarine: samples of yellow-fleshedpeaches (Prunus persica L. Batsch) and yellow flesh nectarines (Prunus persica L. Batsch, var. Nectarina) of four different cultivars from areas of Southern Italy have been analyzed. ToF-SIMS analysis confirmed the presence of Cyanidin and Phosphatidylcholine in the skin of peaches and nectarines, and Cyanidin, Phosphatidylcholine, Oleic Acid and Coniferyl Alcohol in the skin of seed. ToF-SIMS with statistical data analysis is a promising method for thoroughly investigating the chemical composition and variability of food and natural products, allowing to extrapolate information on geographical origin and possible adulteration

    Time:

    Title: Fermentative Production and Statistical Optimization of Xylitol Using Novel Isolates of Candida parapsilosis Strain BKR1 in Indigenously Designed Multiphase Reactor

    Balakrishnaraja R
    Bannari Amman Institute of Technology, India.

    Biography
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    Biography

    Balakrishnaraja R
    Bannari Amman Institute of Technology, India.



    Abstract
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    Abstract

    Balakrishnaraja R
    Bannari Amman Institute of Technology, India.

    Xylitol is a natural polyol and most widely known for its sugar substitute properties in diabetic patients. It is also used against the oral bacterial population. Most fascinating approach for commercial production of xylitol involves the suitable yeast fermentation. In this present investigation, factorials Optimization of these medium and process conditions are studied. Xylitol production by Candida parapsilosis strain BKR1 using Plackett-Burman and RSM are reported in modified minimal medium. The Plackett-Burman screening design reports the significant medium components are Xylose, yeast extract, Potassium Dihydrogen phosphate and magnesium sulphate. Further factorial optimization using face centred central composite design reveals the optimum levels of the significant medium components as Xylose – 104.69 g/l, Yeast Extract – 4.12 g/l; KH2PO4 – 2.84 g/l and MgSO4⋅7H2O – 2.09 g/l. Also the process parameters such as agitation, pH, Temperature and Inoculum level were optimized and validated as Agitation: 107 rpm, pH – 5, Temperature – 29.9ºC, Inoculum level – 1 ml. Bioreactor was designed and a pilot scale study was being carried out.

  • Sessions:
    Genetic Engineering and rDNA Technology

    Time:

    Title: Insulation of novel nitrogen starvation induced promoters in the microalgae Phaeodactylumtricornutum

    Zachor Adler Agmon
    Ben-Gurion University,Israel.

    Biography
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    Biography

    Zachor Adler Agmon
    Ben-Gurion University,Israel.



    Abstract
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    Abstract

    Zachor Adler Agmon
    Ben-Gurion University,Israel.

    Insulation of novel nitrogen starvation induced promoters in the microalgae Phaeodactylumtricornutum

    Sessions:
    Marine Biotechnology

    Time:

    Title: Lipid droplets biogenesis andtrafficking in the microalgae Phaeodactylumtricornutum.

    Zachor Adler Agmon
    Ben-Gurion University, Israel.

    Biography
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    Biography

    Zachor Adler Agmon
    Ben-Gurion University, Israel.



    Abstract
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    Abstract

    Zachor Adler Agmon
    Ben-Gurion University, Israel.

    The understanding and genetic engineering of algal lipid droplets (LD) biogenesis and trafficking hold great promise for the enhanced production of high value compounds or oil in microalgae. It seems that in P. tricornutum, the biogenesis of the LDs begins with several small, scattered LDs which gradually associate into one big LD. By using tubulin-specific cytoskeleton inhibitors, we found that this globule translocation and ultimate fusion can be inhibited. In addition, applying cytoskeleton inhibitors after relocating the LDs inside the cytoplasm, we saw a clear inhibitory effect of the microtubule inhibitor on the return of the LDs to their original location, which suggests that P.tricornutum LDs are connected to the microtubules. Further supporting evidence for this notion was found by tagging α tubulin and β actin with GFP, which demonstrated how the microtubules embrace the LDs, while the β actinis not come with any close interaction with the LDs. We have carry out proteomics on the LDs proteins of the P. tricornutum, to date, the majority of the proteins identified by it, are unknown proteins. Based on our need to isolated and identified proteins who have a role in LDs trafficking, we had to establish our won model; this model is based on the predicted 3D structure of a given protein, and its hypothetical interaction with the cytoskeleton. We will show in-vivo result that support our model. For example, we will show how the HOGP (Peled et al., 2011)is able to hijack the control over the cytoskeleton.

    Sessions:
    Pharmaceutical Biotechnology

    Time:

    Title: Polysaccharide Bacterial Toxins as Anticancer Agents

    Roger A Laine
    Rice University, USA.

    Biography
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    Biography

    Roger A Laine
    Rice University, USA.

    Professor Roger Laine, B.A., University of Minnesota, PhD, Rice University, Houston, Texas (Professor Alan D. Elbein), postdoctorals with Prof. Charles C Sweeley at Michigan State University, and Prof. Sen-itiroh Hakomori, University of Washington. He was Assistant and Associate Professor, University of Kentucky-Medical in Lexington, Kentucky, and then Professor and Chair of the Department of Biochemistry, Louisiana State University, currently Professor of Biochemistry and Chemistry. He was Chief Scientist, Glycomed, Inc., San Francisco, and Founder of biotechnology companies Anomeric, Inc., TumorEnd, LLC (www.tumorend.com), Citrazone, LLC, Enzomeric, LLC, and partner in Glycon, LLC, and has authored 150 publications, and 28 patents.



    Abstract
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    Abstract

    Roger A Laine
    Rice University, USA.

    Busch, (1866, 1868), observed sarcoma patients with nosocomial erysipelas had spontaneous regression of tumors. Intentionally infection of sarcoma patients with erysipelas, showed regression of tumors in patients. Fehleisen, (1882) identified the erysipelas organism Streptococcus erysipelatos (S. pyogenes), repeated Busch’s observations, deliberately infecting cancer patients with injections of S. erysipelatos, finding tumor reduction in 7 of 7 cases. Bruns, (1888), described 3 out of 5 permanent cures of malignancies with erysipelas infections. Roger, (1892) in France, enhanced virulence of erysipelas streptococcus by co-injection in rabbits with Serratia marcescens,. W. B. Coley (1893, 1896, 1909, 1910) used heat killed cultures of both organisms commonly referred to as “Coley’s Toxin” for his treatments. “Coley’s Toxin” achieved clinical successes by Coley and others into the 1930’s. In the 1980’s Hellerqvist, Sundell, et al. isolated polysaccharide toxin from Group B Streptococcus, the causative agent of “Early Onset Disease” in humans. A potent 300kDa polysaccharide from GBS culture filtrate, was protein, LPS free containing lipid and phosphate. (Hellerqvist, 2002). GBS Toxin caused tumor specific capillary damage and tumor regression in rodents, and in a successful Phase I clinical trial in volunteer stage 4 humans (DeVore, et al. 1997), with 33% effectivity. A binding receptor 55kda protein (SP55) (Fu, et al., 2002) was found in humans encoded by the SLC17A5 gene. This receptor was found to be expressed on all tested human tumors in capillary endothelium. A Phase II trial is planned, and a canine cancer trial is in progress.

    Time:

    Title: Native Cell MembraneNanoparticlesSystem for Membrane Proteins

    Youzhong Guo
    Virginia Commonwealth University, USA.

    Biography
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    Biography

    Youzhong Guo
    Virginia Commonwealth University, USA.

    Youzhong Guo was born in Xiangcheng, Henan, China, in 1974. He received the B.S. degree in biology from Henan Normal University, Xinxiang, Henan, China, in 1997. He received the Ph.D. degree in pharmacy/structural biology from the University of Texas at Austin, Austin, TX, U.S.A., in 2010. From 2010 to 2016, he worked with Dr. Wayne A. Hendrickson as a postdoc in Columbia University, New York, NY, U.S.A. In 2016, he joined the Department of Medicinal Chemistry, Virginia Commonwealth University, Richmond, VA, U.S.A. as an Assistant Professor. His current research interests include membrane protein structural biology and structure-based drug discovery.



    Abstract
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    Abstract

    Youzhong Guo
    Virginia Commonwealth University, USA.

    We devised a native cell membrane nanoparticles system, which we applied in a single-particle cryo-EM study of the multidrug exporter AcrB. Lipid-AcrB nanoparticles were prepared directly from membranes without any use of detergents. A 3D reconstruction in C1 symmetry achieved a final density map at 3.2 Å resolution, an atomic model of quasi-C3-symmetric AcrBwas fitted to this map, and the residual density revealed many ordered lipid molecules. Most remarkably, a central cavity between the three transmembrane domains contains a 24-lipid patch of well-ordered bilayer structure. Inner leaflet lipid chains pack in a hexagonal array like that in phosphatidylethanolamine crystal structures, whereas the outer leaflet has highly irregular packing. Protein side chains interact with both leaflets and participate in the hexagonal pattern. The AcrB export mechanism requires reorganization of the lipid bilayer structure. This system should be broadly applicable for membrane protein structural biology and structure-based drug discovery and development.

    Sessions:
    Biotechnology in Healthcare

    Time:

    Title: Continuous multilayered composite PVA hydrogel as cartilage substitute

    Gemma Leone
    University of Siena, Italy.

    Biography
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    Biography

    Gemma Leone
    University of Siena, Italy.

    Gemma Leone is a researcher at the University of Siena. The research activity is focused on the synthesis and the physico-chemical and rheological characterization of natural or synthetic macromolecular based bi-dimensional or three-dimensional surfaces, which can be utilized as cell scaffolds or drug vehicles. Her research fields mainly concern on : I) Development of new polysaccharide –based hydrogels for soft tissue regeneration II) Protein adsorption studies on biomaterials III) Synthesis and physico-chemical and rheological characterization of polyvinylic hydrogels as ophthalmological devices IV) Development of model systems for saccharide based biosensors.



    Abstract
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    Abstract

    Gemma Leone
    University of Siena, Italy.

    Cartilage is a highly organized avascular soft tissue that assembles from nano-to macro-scale to produce a complex structural network. To mimic cartilage tissue, we developed a stable multilayered composite material (MSC), characterized by a tailored gradient of mechanical properties. MSCwas obtained through a multistep procedure. A mixture of PVA and HA nanocrystals (nHA/PVA molar ratio of 0.015) was crosslinked using tri-sodium tri-metaphosphate (STMP), the crosslinking agent, added in a molar ratio 1:1 with PVA (PS11HA)(first layer). The second layer was obtained by crosslinking PVA directly on the surface of first layer without addition of the inorganic phase but with the same PVA/STMP molar ratio of the first layer (PS11). The same procedure was then applied to crosslink a third layer, which was produced with a greater PVA/STMP molar ratio (2:1). MSC can be considered a promising potential substitute for damaged cartilage tissue, since it mimics the gradient of water content and rheological properties strictly comparable with those of cartilage in terms of complex modulus (G*: 0.032±0.003 MPa; cartilage: G*: 0.03±0.003 MPa) and recovery (70% recovery after just 0.1 s). The presence of nano-hydroxyapatite in its bottom layer stimulate the adhesion to bone, whereas the uppermost soft layer represents an ideal environment for interaction with cartilage guaranteeing a lubricant action as confirmed by the good cytocompatibility shown by MSC (layer PS21) and MSC (layer PS11HA) towards chondrocytes and osteoblasts, respectively, and by the increased BALP production in samples containing nHA in comparison with samples without nHA.

    Time:

    Title: Development of Low Cost Nano Bioadsorbent Composite Materials for Pharma Waste Treatment

    Lingayya Hiremath
    R.V College, India.

    Biography
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    Biography

    Lingayya Hiremath
    R.V College, India.

    Dr. Lingayya Hiremath, had awarded Ph.D in Biotechnology in year 2004 from Gulbarga University, Gulbarga. He is currently working as Asst. Professor, Dept. Biotechnology, R.V. College of Engineering, Bengaluru, and Karnataka India. He received Young scientist state award from VGST, Govt. of Karnataka in year 2014. He had published more than 20 research papers in reputed national and International journals. He given invited talk in National and International forms such as UB Technological University, Prague Czech Republic. He has been guiding UG/M.Tech and Ph.D students in the various areas in Biotechnology. He has research interest in the field of Environmental biotechnology, Nanotechnology and Clean Energy Technology. He had received funds from various funding agencies to carryout research activities. Recently, he received outstanding “Project of the year” award from Karnataka State Council for Science and Technology, Indian Institute of Science Bengaluru. He had received Funds to setup R&D Centre on “Waste to Clean Energy Lab” by Karnataka Council for Technological Upgradation at R.V. College of Engineering, Bangalore.



    Abstract
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    Abstract

    Lingayya Hiremath
    R.V College, India.

    Medication disposal is alarming issue today and gaining more and more awareness from the healthcare professionals as well as consumers. Pharmacists have the potential to be on the forefront of this movement as healthcare professional and pharmacists are in admirable position to educate patient about safe drugs disposal. Proper patient counseling on safe medication disposal can make a significant difference to public health and environment. A practical approach should be there to incorporate this important issue in the curriculum as the need of the hour. Also establishment of cost-effective and acceptable government run collection and disposal systems is necessary. There should be some norms and stringent guidelines for same. Careful and proper disposal of medications can help to decrease environmental load of drugs. The role of biotechnology for disposal and treatment techniques for medical waste at cost-effective methods will be discussed. All multidisciplinary stake holders, governments, NGOs, physician, pharmacist, patients and public should work together hand in hand to reduce burden of unused and expired medicines on ecosystem. Scientific waste management strategy is needed to ensure health and environmental safety.

    Time:

    Title: The casein kinase 2 (CK2) and PIm kinase as a target for anticancer therapy

    Maria Bretner
    Warsaw University of Technology, Poland.

    Biography
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    Biography

    Maria Bretner
    Warsaw University of Technology, Poland.

    Maria Bretner received M.S. degree in chemistry from the Faculty of Chemistry , University of Warsaw in 1974 and Ph.D in biological sciences from the Institute of Biochemistry and Biophysicsin 1997.She completed postdoctoral studies at the University of Maryland Baltimore County, USA. (1997-1999). She worked at the IBB PAS developing the methods of new inhibitors synthesis of Thymidylate Synthase, HIV RT andHCV helicase. Since 2007 she is working at the Warsaw University of Technology,Faculty of Chemistry .Research areas include chemistry andenzymology, biocatalysis, biochemistry of the cancer processes. Co-author of 69 scientific papers.



    Abstract
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    Abstract

    Maria Bretner
    Warsaw University of Technology, Poland.

    Protein kinases CK2 and PIM belong to serine/threonine kinases that are involved in the regulation of number of signalling pathways. They are upregulated in multiple cancers, including lymphoma, leukemia, breast, prostate, head and neck cancers and act as repressors of apoptosis, contributing to chemoresistance. Over 30 drugs, that target kinases, have been approved for clinical use over the past decade, and hundreds more are undergoing clinical trials, among them are: specific inhibitor of CK2-CX4945 and few specific inhibitors of PIM family e.g. AZD1208, CX-4595, SGI-9481 or LGB321. But their efficiency is not satisfactory so the increasing attention has been recently focused on the significant role of dual kinase inhibitors. Until now a few examples of the benzimidazole derivatives, which affect the activity of both kinases, CK2 and PIM1 have been known. We designed and synthesized novel 4,5,6,7- tetrabromo-1H-benzimidazole (TBBi) and 4,5,6,7- tetrabromo-1H-benzotriazole (TBBt) derivatives with alkylamine substituents. To test the inhibitory properties recombinant human kinase CK2α, CK2 holoenzyme, and kinase PIM1 was obtained in E. coli bacterial expression system. The most promising compound, the 3- (4,5,6,7-tetrabromo-2 methyl-1H-benzimidazol-1-yl) propan-1-amine (14b) inhibited the activity of CK2 with an IC50 of 0.35 M and PIM1 0.15 M. Furthermore, the influence of new active dual inhibitors on the cell viability was evaluated and EC50 determined for 14b with the use of CCRF-CEM, MCF-7 and PC3 cell lines were in the range of 2-4 M. This work was supported by an NSC Poland grant 2014/13/B/NZ7/02273 and by WUT.

    Sessions:
    Poster Presentations

    Time:

    Title: Identification of bread wheat seed-specific bZIP transcription factors binding sites by genome-wide in vitro binding analysis

    koushik Shah
    National Agri - Food Biotechnology Institute, India.

    Biography
    χ

    Biography

    koushik Shah
    National Agri - Food Biotechnology Institute, India.



    Abstract
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    Abstract

    koushik Shah
    National Agri - Food Biotechnology Institute, India.

    Basic leucine zipper (bZIPs) are dimeric sequence-specific transcription factors (TFs) that are unique to eukaryotes and play an important role in various biological processes including seed development and maturation, plant immunity and defense, and biotic and abiotic stress. In humans and other vertebrates bZIP TFs DNA binding sites gene promoters are well characterized but not much is known in plants. Problem is more pronounced in economically important crop like wheat. Recently an ordered draft sequence of hexaploid wheat (Triticum aestivum) genome and transcriptome analysis has opened new opportunities to study the roles of TFs proteins in gene regulation in wheat. Particularly, we are interested in analyzing the structure and functions of bZIP TFs of wheat. In last few years there has been a spurt in breakthrough technologies that identify TF-binding sites in whole genome (e.g. PBM, ChIP-seq, Bind-n-seq/DAP-seq). The new protocols are helping in identify hitherto unknown binding sites of TFs both in vivo and in vitro. We have cloned seed- specific bZIPs and adapted high-throughput bind-n-seq methodology to identify their genome- wide binding sites. Bind-n-Seq is a simple and robust method in which bZIPs are incubated with bar coded random oligonucleotides libraries (70 mer) with random binding sequences. Pure bZIP bound oligonucleotides are isolated and are sequenced using illumina platform. Based on abundance binding sites are scored. One of significant advantage of bind-n-seq over other method is that large numbers of binding sites are captured for each bZIP that is used in constructing transcription factor binding landscape.

    Time:

    Title: Development and characterization of transgenic pigeon pea plants carrying Osruvb gene against salinity stress tolerance

    Rakshita Singh
    CCSHAU,India.

    Biography
    χ

    Biography

    Rakshita Singh
    CCSHAU,India.



    Abstract
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    Abstract

    Rakshita Singh
    CCSHAU,India.

    Stress which arises due to environmental parameters such as salinity, drought, high temperature and cold disrupt the normal metabolism of plants. Almost all abiotic stress conditions generate osmotic stress in the plants. Salinity being a very vital problem for the survival of crops leads to major losses in crop productivity. Overexpression of DNA helicases like PDH 45, PDH 47 leads to abiotic stress tolerance (salinity tolerance in tobacco). However, the role of overexpression of RuvB, which is also a DNA helicase, in abiotic stress tolerance in plants has not been reported so far. Therefore, we have developed transgenic pigeonpea plants overexpressing OsRuvB gene, working under the control of CaMV35S promoter to analyse the effect of this gene on plants under saline conditions. The transgene integration in putative T0 plants has been confirmed through PCR analysis and transformation efficiency of 35-40% has been observed. The transgene integration has also been confirmed in T1 plants through PCR and these plants have been exposed to salinity stress. The physio-biochemical parameters such as relative water content, chlorophyll content, membrane stability test, proline content etc. have been studied to assess the tolerance level of the transgenic plants. The PCR positive transgenic plants are being analysed through southern hybridization and real time PCR to determine the transgene copy number.

    Time:

    Title: The effect of stress on behavior and immunity in Wistar rats

    Sansri Soraya
    University Badji Mokhtar Annaba, Algeria.

    Biography
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    Biography

    Sansri Soraya
    University Badji Mokhtar Annaba, Algeria.



    Abstract
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    Abstract

    Sansri Soraya
    University Badji Mokhtar Annaba, Algeria.

    Stress is a major current problem both in humans and in animals and implement strategies to limit sometimes adverse effects. In addition, exposure to stress causes behavioral and immune disorder in rodents. Experimentally, this modification is based on the intensity and type of exerted stress. The objective of this work is to study the effect of three types of stresses, acute restraint, and predation by separation to assess immune and behavioral changes in the Wistar rat. Comparison between the three types of stresses, Behavioral and adaptive changes in the rat are an attempt to identify the behavioral parameters evaluated from the open fields andmaze. Our results on stressed rats showed the following: Increased anxiety with onset of depression evaluated in tests in an elevated cross maze and open field. Impairment of spatial memory. Disturbance of the immune system cells and humoral response (monocytes and lymphocytes).

    Time:

    Title: Study of the in vitro germination of immature embryos of orange trees (Citrus sinensis)

    Karim Mahmoudi
    Ibn Tofail University,Morocco.

    Biography
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    Biography

    Karim Mahmoudi
    Ibn Tofail University,Morocco.



    Abstract
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    Abstract

    Karim Mahmoudi
    Ibn Tofail University,Morocco.

    Oranges constitute the major part of the production of citrus fruit, which is the most important fruit group in international trade.The creation of new triploid hybrids via the rescue of immature embryos allows diversification of the varietal profile of orange trees. The objective of this study is to optimize the in vitro germination of immature embryos according to the chemical composition of four in vitro culture media in two varieties of orange trees (Pineapple and Parson Brown). At the maturity stage, the fruit was harvested and the extracted seeds were classified according to their size. Only small or flat seeds were cultured in a base medium of Murashig and Tuker (MT) under sterile conditions. The different growth regulator concentrations were tested to obtain the best medium for seedling development: M1 (MT + 1 mg / l GA3), M2 (MT + 1mg / l Kenitin + 0.5 mg / l BAP + 0.1 mg / l ANA), M3 (MT + 25 mg / l adenine sulphate), M4 (MT + 0.5 mg / l Kenitin + 0.5 mg / l BAP + 1 mg / l GA3). For both Orange varieties Pineapple and Parson Brown, the germination rate is maximum in M3 medium respectively at percentages of 100% and 90%. varieties between 6 and 7 days. With respect to growth rate (mm / week), both varieties knew a variation in the four media. Similarly, the maximum acclimation rate in the M1 medium is 80% and 90% respectively for the Pineapple and Parson Brown varieties. In general, the smaller the embryos, the more sensitive they are to the composition of the culture medium. It is therefore essential to optimize the components of the medium to promote their growth and their in vitro developments. Therefore, the medium M1 (MT + 1 mg / l GA3) remains the best to promote good germination in short time and a better acclimatizationrate.

    Time:

    Title: Appraisal of microcarrier suspension in a single-use shaken bioreactor with conical bottom

    Gregorio Rodriguez
    University College London, UK.

    Biography
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    Biography

    Gregorio Rodriguez
    University College London, UK.



    Abstract
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    Abstract

    Gregorio Rodriguez
    University College London, UK.

    The pharmaceutical industry is at the forefront of the production of drug products using mammalian cell-based cultures, some of which rely on anchorage dependant cells, with single-use technology bioreactors gaining prominence. Whilst often adapted to suspensioncultures, hybridoma and other cells give increased product yields when culturedattached to a substrate, such as microcarriers, resulting in smaller fermentation runs at higher densities. Microcarriers are also used in tissue engineering andcell for therapy culture. Stem cells are adherent-dependantcells and traditional 2-dimensional static cultures rely on disposable multilayervessels, which have become the common route for stem cell expansion. It has been a recent industry trend to use shaken bioreactors at large scale in the upstream process, performing mammalian cell culture in single-use bags which eliminate the need for cleaning in place, offer flexibility and lower production down-times. Whilst currently still to be fully developed, large scale production of stem cells would require the use of 3-dimensional dynamic culture methods by employing microcarrier technology, asdemonstrated by Frauenschuh et al. (2007). Microcarriers consist of sphericalbeads with a size of 100-300 μm, and can be made of a wide variety of materials, amongst others: plastics, glass, acrylamide, silica, silicone rubber, cellulosedextran and collagen. Medium to large scale single-use devices would greatly benefit the consistency of stem cell culture whilst maintaining potency. Whilst most studies have focused on investigating optimal compositions of microcarrier materials, their concentration and cell culture media, little researchhas been undertaken on the engineering aspects of microcarrier use.The aim of the work is to characterize the suspension dynamics of microcarriers in a cylindrical orbitally shaken bioreactor (OSR) with conical bottoms of different heights. This study builds upon previous works of the research group (1-4) for a flat bottom reactor, where increases in Froude number were found to determine a mean flow transition which was found to be instrumental in determining the just-suspended speed. The dynamicsof solid suspension is studied using commercially available Cytodex-3,stained with trypan for improved visual contrast and image acquisition. The experimental procedure allows to estimate not only the speed required for the solids to lift from the vessel bottom, but also the conditions at which a homogenous distribution of microcarreirs is obtained.Preliminary results indicate that the presence of the conical bottom improves solid suspensionby requiring lower agitation rates for the microcarriers to lift fromthe bottom completely. The critical Froude, which determines theflow type controlling the bioreactor, can be used to scale thesuspension of microcarriers in OSRs.

    Time:

    Title: The Generation of g-secretase inhibitor-loaded PLGA-Fe3O4- Magnetic Nanoparticles

    Roa Bashmail
    Dublin City University, Ireland.

    Biography
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    Biography

    Roa Bashmail
    Dublin City University, Ireland.



    Abstract
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    Abstract

    Roa Bashmail
    Dublin City University, Ireland.

    Cardiovascular disease is the number one killer in Ireland and the wider EU. A hallmark of the disease is the obstruction to blood flow due to the build-up of vascular smooth muscle (SMCs)-like cells within the vessel wall. Treatment options include percutaneous transluminal coronary angioplasty (PTCA) and the insertion of a stent – a metal mesh tube – into the obstructed vessel to keep it open. However, the vessel can become re-occluded due to the accumulation of SMC-like cells within stented area. The introduction of the 1 st generation drug-eluting stents (DES) has resulted in a paradigm shift for the treatment of in-stent restenosis with significant improvement in therapeutic outcomes. However, while polymer-coated DES have significantly reduced the incident of in-stent restenosis, current DESs lack the fundamental capacity for (i) adjustment of the drug dose and release kinetics and the (ii) ability to replenish the stent with a new drug on depletion. This limitation can be overcome by a strategy combining magnetic targeting via a uniform field-induced magnetization effect and a biocompatible magnetic nanoparticle (MNP) formulation designed for efficient entrapment and delivery of specific drugs that target the resident vascular stem cell source of the SMC. Magnetic nanoparticles (MNP’s) containing magnetite (Fe 3 O 4 ) were fabricated, polymer coated with poly(DL-lactide- co-glycolide) polyvinyl alcohol [PLGA-PVA] and loaded with a -secretase inhibitor (GSI) of Notch signalling, DAPT using an oil in water emulsification technique. The free GSI’s and GSI- loaded MNP’s were assessed for drug release, the efficacy at controlling mesenchymal stem cell (MSC) growth (proliferation and apoptosis) and inhibiting myogenic differentiation under magnetic and non-magnetic conditions. The DAPT-loaded MNPs had an average hydrodynamic diameter of 351 d.nm Up to 40% of drug was released from MNPs within 48 h rising to 65% after 1 week under magnetic conditions. The Notch ligand, Jagged1 increased Hey1 mRNA levels and promoted myogenic differentiation of MSCs in vitro by increasing SMC differentiation markers, myosin heavy chain 11 (Myh11) and calponin1 (Cnn1) expression, respectively. This effect was significantly attenuated following treatment of cells with MNP’s loaded with DAPT when compared to unloaded MNP’s. Notch GSI loaded magnetic nanoparticles are functional at targeting vascular stem cells in vitro.

    Time:

    Title: Synergistic effect of cholesterol-vitamin E, solubilized in cyclodextrins, on frozen-thawed bovine semen

    Khellouf Allaeddine
    Abderrahmane-Mira-University,Algeria.

    Biography
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    Biography

    Khellouf Allaeddine
    Abderrahmane-Mira-University,Algeria.



    Abstract
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    Abstract

    Khellouf Allaeddine
    Abderrahmane-Mira-University,Algeria.

    Cryopreservation of sperm has been developed considerably in the recent years. However, during the different phases of the cryopreservation process spermatozoa are affected by various stresses. The aim of this study was to minimize this damage, particularly at the membrane level by supplementing the freezing extender medium with bioactive molecules, vitamin E and cholesterol. The two molecules are supposed to act in a complementary manner, cholesterol to reinforce the cell membrane and to fight against the cold shock, and vitamin E to fight against the oxidative stress. As the two molecules are lipophilic, they were both preloaded in cyclodextrins to enhance their solubility. The two molecules were used alone or in association. Sperm mobility (using CASA), sperm viability (using HOST) and levels of lipid peroxidation (using TBARs) were used to analyze sperm quality. The post-thawed results showed a significant protection of all sperm parameters when vitamin E and cholesterol were used simultaneously with 57.69±0.82 µm/s, 39.87±6.3 %, 0.046±0.12 nmol/108, for VCL, viability, and oxidative stress status, respectively. Associating cholesterol and vitamin E, both preloaded in cyclodextrins, seems to offer a real opportunity to improve bovine semen quality after freezing-thawing process.

    Time:

    Title: EhLINE1 and EhSINE1 expression profiling using Next Generation Sequencing reveals uneven transcript distribution and antisense expression of translationally-silent regions

    Devinder Kaur
    Jawaharlal Nehru University, India.

    Biography
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    Biography

    Devinder Kaur
    Jawaharlal Nehru University, India.



    Abstract
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    Abstract

    Devinder Kaur
    Jawaharlal Nehru University, India.

    Entamoeba histolytica is a primitive parasitic protist. 11% of its genome is comprised of retrotransposons (EhLINEs and EhSINEs). LINE and SINE copies are generally maintained in a transcriptionally silent state with few copies being active. To determine the transcriptional status of E. histolytica retrotransposons, RNA-Seq was carried out in triplicate and reads corresponding to EhLINE1 and EhSINE1 were elucidated. Of total 948 copies of EhLINE1, and 493 copies of EhSINE1, 41 LINE1 and 129 SINE1 copies were shown to be transcribed. Of 41 expressed LINE1, 20 were full length while the rest had deletions/truncations. Uneven read distribution of ORF1 and ORF2 was observed with ratio of 1:40 respectively which was validated by northern with 1.5 kb bands from both ORF1 and 2 probes, ORF2 band is much brighter. Read-through transcription was observed for 26% LINE1 in both direct and antisense direction. Further, we looked for antisense expression of EhLINE1 and EhSINE1. Interestingly, 80% of EhLINE1 and 40% of EhSINE1 expressed copies showed antisense expression with LINE1 at a significantly high level compared with sense strand. Only the reverse transcriptase (RT) and Endonuclease (EN) region of LINE1 showed antisense expression whereas in SINE1 it was seen throughout the element. According to the current paradigm of LINE transcription, these elements are transcribed into a single polycistronic transcript from a 5’end internal promoter. Our data suggest a complex transcriptional profile whereby transcripts corresponding to different regions of EhLINE1 accumulate to different levels, and antisense transcripts presumably attenuate translation of some of the transcripts.

    Time:

    Title: Production and Characterisation of extracellular chitinase from a novel isolate Chitinophaga sp. S167

    Sonia Sharma
    Punjab University,India.

    Biography
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    Biography

    Sonia Sharma
    Punjab University,India.



    Abstract
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    Abstract

    Sonia Sharma
    Punjab University,India.

    Chitinases cleave β-1,4 N acetyl glucosamine linkages and thus have the ability to degrade chitin in cell wall of fungi and exoskeleton of insects. They are potential antifungal, insecticidal and nematacidal agents. Chitinases have been isolated from bacteria, fungi, plants, insects, crustaceans, animals and humans. Based on the site of action on the substrate, they are classified as exochitinases or endochitinases. An isolate S167 (GeneBank Acc. No. KP017541) showing 98.62%, 98.47 and 97.78% 16S rDNA similarity to Chitinophaga ginsengisoli, C. filiformis and C. pinensis respectively, on EzTaxon was isolated from soil rich in organic decaying matter. Chitinophaga sp. S167 shows chitinolytic activity and produces extracellular chitinase. The chitinase from Chitonophaga sp. S167 inhibited Cladosporium sp., Alternaria sp. and Fusarium sp. The enzyme was found to be optimally active at 40⁰C and pH 6. The chitinase was maximally induced at 72 hours by 1.5% swollen chitin when incubated in the medium of pH 8 at 35°C. The enzyme was purified using ion exchange and hydrophobic interaction chromatography. The purity of the enzyme was checked by SDS-PAGE which showed a band with an apparent molecular weight of 50 kDa and its activity was confirmed by zymography. Further studies are being carried out to characterize the enzyme and determine its substrate specificity.

    Time:

    Title: 2,3-Diaryl indenone and 2-Chloro-3-Amino indenone derivatives as selective inhibitor of DNA repair enzyme AlkB and AlkBH3

    Richa Nigam
    IITH, India.

    Biography
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    Biography

    Richa Nigam
    IITH, India.



    Abstract
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    Abstract

    Richa Nigam
    IITH, India.

    Human AlkB homologue-3 (AlkBH3) is a DNA repair enzyme that demethylases N1-methyladenine and N3-methylcytosine base lesions. AlkBH3 is an ortholog of bacterial AlkB and also known as prostate cancer antigen-1 (PCA-1) and known to express abundantly in several types of cancers, including prostate cancer. Because of its immense biological and clinical significance, extensive efforts are being directed in developing selective inhibitors for AlkBH3. Here we report synthesis, screening and evaluation of panel of arylated indenone and 2-chloro-3-amino indenone derivatives as new class of specific inhibitors of AlkB family of DNA repair enzymes. An efficient synthesis of 2,3-diaryl indenones from 2,3-dibromo indenones was achieved via Suzuki-Miyaura cross-coupling. Further, synthesis of 2-chloro-3-amino indenone derivatives was achieved from 2,3-dichloro indenones via addition elimination method. Using a robust quantitative assay, we have obtained few inhibitors that showed a unique competitive inhibition mechanism against DNA substrate and a mixed inhibition against 2OG co-substrate. These AlkBH3 inhibitor rendered human cells hypersensitive to exposure to DNA/RNA damaging alkylating agent. This discovery is the first report of an indenone derivative as inhibitors targeting bacterial and human DNA alkylation repair and provides a framework from which second-generation indenone derivatives may be developed.

    Time:

    Title: Production of High Electricity from Plant Using Genetic Engineering Technique

    Tarhima Jahan Jerin
    Mawlana Bhashani Science and Technology University, Bangladesh.

    Biography
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    Biography

    Tarhima Jahan Jerin
    Mawlana Bhashani Science and Technology University, Bangladesh.



    Abstract
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    Abstract

    Tarhima Jahan Jerin
    Mawlana Bhashani Science and Technology University, Bangladesh.

    In the world 1.4 billion people have accessed electricity.Our aim is to get a large amount of electricity from a plant to meet up the storage of electricity of the world. Living plants can be used to produce electricity in the process of photosynthesis and with the help of bacteria in the soil. By easy placing two electrodes in the soil we can produce literally green electricity. We have heard about an electric fish called Electric Eel(Electrophorus electricus). It is most famous for its ability to generate strong voltage discharge up to̴ 600 volts. The shocking skills depend on a gene called Scn4a.This study also represent the first analysis of miRNAs displaying electric organ specific expression patterns including one novel miRNA highly over expressed in all the electric organs of Electric Eel.We are using pea plants in this process. By using recombinant DNA technology we will insert the Scn4a gene with the miRNA in the root cell of plants. As a result the root cells of the plant will produce electricity.The gene we insert will show its character of electricity.The electricity will flow down through the electrode,power harvester and cathode and then it will be stored in theIPS battery.The rate of electricity production will be about 650 volts.In the houses 220 volts electricity is needed.So we can store a large amount of electricity and supply it.

    Time:

    Title: Enhanced Production of L-glutaminase from Bacillus licheniformis by Taguchi DOE

    Hare Ram Singh
    BITS,India.

    Biography
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    Biography

    Hare Ram Singh
    BITS,India.

    Dr. Hare Ram Singh having a long experience in the field of bioprocess engineering. He is actively engaged in the bioprocess development for the industrially important biomolecules using the microbial system. He has expertise in the process optimization, downstream processing and mathematical modelling of the bioprocess. By profession he is an academician cum researcher and presently serving as a Assistant Professor in the Birla Institute of Technology, Mesra, India.



    Abstract
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    Abstract

    Hare Ram Singh
    BITS,India.

    L-glutaminase (L-glutamine amidohydrolase, EC 3.5.1.2) is an important enzyme due to its property such as, it enhances the flavour of food and can also act as an anti-leukemic agent and as a biosensor. L-glutaminase is produced by micro-organisms like bacteria, fungi, yeast and including humans and animals. L-glutaminase hydrolyses glutamine to glutamic acid and ammonia. The objective of the present investigation is to qualitative and quantitative screening of potential L-glutaminase producers. The primary screening of L-glutaminase is performed by rapid plate assay method on the basis of pH dependent analysis. Bacillus licheniformis is observed as the maximum producer of L-glutaminase, which is then used for the further investigation. The secondary screening is performed under optimized conditions. Taguchi orthogonal method of optimization of six factors viz. Carbon source, Nitrogen source, Salts, Incubation period, pH and Temperature was used for the maximum production. The maximum enzyme production of L-glutaminase was observed at dextrose (2.5g/L), L-glutamine (0.9g/L), MgSO4.7H2O (0.4 g/L), NaCl (0.35 g/L), KH2PO4 (2.5 g/L), CaCl2.2H2O (0.9 g/L), Na2HPO4.2H2O (5 g/L), pH 7.0 at 370 C. An optimized enzyme production ensures high profitability and authentic significance in terms of its usage.

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