International
Biotechnology and Research Conference

April 25-27, 2018 | Rome, Italy

Program Schedule

  • Keynote Speaker

    Time:
    09:30 - 10:15

    Title

    Title: Cold-active lipases: Characterization and Structure Elucidation

    Raja Noor Zaliha Raja Abd Rahman
    University Putra Malaysia, Malaysia
    Biography
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    Biography

    Raja Noor Zaliha Raja Abd. Rahman obtained her B.Sc. (Hons)-Microbiology from Universiti Sains Malaysia in 1989 and her M.S. in Microbiology from Universiti Putra Malaysia in 1994. She obtained her Doctor of Engineering in Molecular Biology from Kyoto University, Kyoto, Japan in 1998. She is currently the Professor and Head of Enzyme and Microbial Technology Research Centre, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia. In recognition of her scientific findings, she has been awarded patents, prizes and commendations and has derived personal satisfaction from the success of her graduate students, her participation on national and international governmental projects and not least, having a bacterial species named after her.



    Abstract
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    Abstract


    Keynote Speaker

    Time:
    10:30-11:15

    Title

    Title: The CITAS Story on a Multidisciplinary Approach in Disease Detection in Agriculture for Developing Countries

    Maria Regina Justina Estuar
    Ateneo de Manila University, Philippines
    Biography
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    Biography

    Maria Regina is a Fulltime Professor at the Department of Information Systems and Computer Science, Ateneo de Manila University. She currently manages two laboratories: Ateneo Java Wireless Competency Center and the Ateneo Social Computing Science Laboratory where research focus is on in the design and development of social, mobile and wireless systems to understand and model collective behavior and capacity. She teaches Social Computing, Information Systems for Disaster and Health.



    Abstract
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    Abstract


    Sessions:
    Industrial and Microbial Biotechnology & Biotechnology in Agriculture & Biosensors and Biomarkers & Environmental Biotechnology and Biodiversity, Waste Water Treatment

    Time:
    11:15-11:40

    Title: New Opportunities of Biocatalysis in Industrial Biotechnology

    Luis Fonseca
    Institute for Bioengineering and Biosciences, Portugal.

    Biography
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    Biography

    Luis Fonseca
    Institute for Bioengineering and Biosciences, Portugal.

    Luis P. Fonsecais Associate Professor with Habilitation and Ph.D. of Department of Bioengineering at Instituto Superior Tecnico (I.S.T.) of University of Lisbon (U.L.), Portugal and Senior Research Scientist (PI) at the Institute for Bioengineering and Bioscience (iBB) at I.S.T. Prior positions included Chemical Engineer at Hovione, Lda (1985-1986), a Visiting Researcher at CIPAN, Lda (1987-1988), Post-Doc at The School of Biochemistry and Molecular Biology, University of Leeds (1997-1998), Visiting Scholar at Chemical Engineering Department, University of California, Berkeley (2004-2005), and at Institute of Chemistry at University Federal of Rio Grande do Sul, Brazil (2011-2012) and São Carlos at University of São Paulo, Brazil (2015 and 2017).



    Abstract
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    Abstract

    Luis Fonseca
    Institute for Bioengineering and Biosciences, Portugal.

    Chemical products continue to have vital importance to the world economy and are essential to the health, food and consumer product industries.However, theyled to environmental damage and a low public perception of chemical industry. For that reason, there is increasing pressure from both society and Governments for development of new industrial processes to become more sustainable, reducing waste and preventing the use of toxic substances. Consequently, there is a huge opportunity for research and implementation of new cleaner and green processes in the field of industrial biotechnology namely Biocatalysis and Biotransformation. Within this scope, Luis P. Fonseca has focus his research interests on application of biocatalysis in green processes, and development and implementation of reaction media using basically water due to higher enzyme stability and also increasing environment concerns. The design of oil-in-water emulsions (also named miniemulsions) have allowed the production of a high range of high-value products from flavors and fragrances, emollients, polymers, nutraceuticals, and specialty and fine chemicals that led to the development ofAromase technology. This technology is characterized by the enzymatic catalysis, utilization of renewable material resources, high energetic efficiency, no toxicity by using reactions based in water, prevention of waste and sub-products,, among many others advantages. Later modification of the miniemulsion composition evolved to NanoLipCar technology with main goal to design and processing of new drug delivery carriers for encapsulation of cosmeceuticals, nutraceuticals and pharmaceuticals at form of Lipid Nano-Emulsions (LNEs) and Lipid Nano-Particles (LNPs) in this last using long fatty acids that solidify at higher than 37°C.

    Time:
    11:40-12:05

    Title: Immobilization of ARM Lipase for Industrial Use

    Raja Noor Zaliha Raja Abd Rahman
    University Putra Malaysia, Malaysia

    Biography
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    Biography

    Raja Noor Zaliha Raja Abd Rahman
    University Putra Malaysia, Malaysia

    Raja Noor Zaliha Raja Abd. Rahman obtained her B.Sc. (Hons)-Microbiology from Universiti Sains Malaysia in 1989 and her M.S. in Microbiology from Universiti Putra Malaysia in 1994. She obtained her Doctor of Engineering in Molecular Biology from Kyoto University, Kyoto, Japan in 1998. She is currently the Professor and Head of Enzyme and Microbial Technology Research Centre, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia. In recognition of her scientific findings, she has been awarded patents, prizes and commendations and has derived personal satisfaction from the success of her graduate students, her participation on national and international governmental projects and not least, having a bacterial species named after her.



    Abstract
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    Abstract

    Raja Noor Zaliha Raja Abd Rahman
    University Putra Malaysia, Malaysia

    ARM lipase was isolated from Geobacillus sp. strain ARM, holds many potentials for industrial applications as it is thermostable, organic solvent tolerant, 1,3-regioselective. It prefers medium and long chain fatty acids as substrate. In this study, the enzyme was overexpressed in Escherichia coli system and then purified using affinity chromatography. The purified enzyme was immobilized in gelatinized sago solution and spray-dried by entrapment technique in order to enhance the enzyme operational stability for handling at high temperature and also for storage. The physical characteristic of the immobilized enzyme was studied by scanning electron microscopy, surface area and porosity. The immobilized ARM lipase showed good performance at high temperature. The immobilized enzyme could be stored at for few months without loss of activity. Collectively, the immobilized lipase shows promising capability for industrial use.

    Time:
    12:05-12:30

    Title: Towards the Development of a Multidimensional Multisensor Spatiotemporal Model for Disease Detection and Spread

    Maria Regina Justina Estuar
    Ateneo de Manila University, Philippines

    Biography
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    Biography

    Maria Regina Justina Estuar
    Ateneo de Manila University, Philippines

    Maria Regina is a Fulltime Professor at the Department of Information Systems and Computer Science, Ateneo de Manila University. She currently manages two laboratories: Ateneo Java Wireless Competency Center and the Ateneo Social Computing Science Laboratory where research focus is on in the design and development of social, mobile and wireless systems to understand and model collective behavior and capacity. She teaches Social Computing, Information Systems for Disaster and Health.



    Abstract
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    Abstract

    Maria Regina Justina Estuar
    Ateneo de Manila University, Philippines

    Conductivity, amount of light, and air temperature. Weather data include air temperature, relative humidity, rainfall, and air pressure. Mobile phone data include leaf images captured from infected and non-infected sites. Weather data is used to impute for sWith the increasing availability of low cost devices in monitoring crops, small scale farmers, especially in developing countries, now have the opportunity to access Smart farming technologies in monitoring crops. The Philippines is the 3rd largest export producer of Cavendish banana. There are also over 180 small to medium scale banana plantations, nationwide. This study attempts to develop a predictive model for the the early detection of Fusarium oxysporum f. sp. cubense Tropical Race 4 or more commonly known as Panama Wilt, a soil born fungi based disease that greatly affects the production of bananas in the country. The study attempts to test whether a multidimensional approach, using multi-sensor data can be used to develop a geospatial predictive model for early detection of disease. Data is extracted from three sources including soil sensors, weather station, and mobile phone. Soil data include data coming from home grown pH sensors calibrated to work with an off the shelf soil sensor that captures soil temperature, soil moisture, soil data that was corrupted or not captured for some time period. A resulting ARIMAX model has been created that had an RMSE of roughly 16 epidemic incidences for short-term forecasting. Image data has also been explored to generate structural features of possibly infected banana leaves through an autoencoder, which allows the feature extraction for a single class. This results to latent variables that efficiently encode significant information in the images. The success rate of the autoencoder is at 91% for healthy specimens, while only at 78% for infected samples. Integrating the various data extracted from both time-series and image-based models, a linear model that incorporates both spatial and temporal factors can be created to quantify and predict disease spread.

    Time:
    12:30-12:55

    Title: A Framework for Real time Detection and Monitoring of Plant Diseases: A Case Study on Use of Web and Mobile Applications on Foc TR4

    Marlene M. De Leon
    Ateneo de Manila University, Philippines

    Biography
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    Biography

    Marlene M. De Leon
    Ateneo de Manila University, Philippines

    Marlene De Leon earned her Ph.D. in Computer Science degree from the Ateneo de Manila University, Philippines. She currently works as a full-time Associate Professor at the Department of Information Systems and Computer Science (DISCS), where she teaches Software Engineering, Systems Analysis and Design, and Database Management. She serves as one of the mentors in the Ateneo Social Computing Science Laboratory and the Ateneo Java Wireless Competency Center where disaster management systems and healthcare management systems are developed.



    Abstract
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    Abstract

    Marlene M. De Leon
    Ateneo de Manila University, Philippines

    The proliferation of Internet of Things (IoT) devices and cyberphysical structures allowed mobile and web applications to be used for early detection and monitoring of plant diseases. The Cloud-based Intelligent Total Analysis System (CITAS) is an intelligent farming system composed of mobile applications, web applications, and wireless sensor networks (WSNs). It aims to detect early and monitor the presence of Fusarium oxysporum cubense TR4 (Foc TR4) on Cavendish bananas cultivated in the Philippines using modeling and analysis. CITAS Mobile was developed as a data collection tool for farmers. Data collected include: 1) plant leaf images; 2) qualitative plant characteristics; 3) soil WSN parameter readings (e.g., humidity, pH); and 4) GPS coordinates. CITAS Mobile analyzes plant leaf images in real-time to determine the presence or absence of the disease. Data collected by the mobile application are sent to the CITAS Server through its Application Program Interface (API) end-point via mobile internet connection for multidimensional modeling and analysis. CITAS Web provides farm owners and researchers access to historical data collected by CITAS mobile. CITAS Web has the following features: 1) data-entry of soil sample characteristics; 2) database display of farms and plants; 3) historical trend visualization of soil WSN parameter readings; and 4) visualization of multidimensional analysis results involving plant leaf images, microscopic soil images, soil WSN parameter readings, and soil physico-chemical and nutrient characteristics. CITAS provides a framework for collection, management, analysis, and visualization of plant disease spread in a geospatial interface. Currently, total analysis allows for early detection and monitoring of Foc TR4. Farmers, farm owners, and researchers may use CITAS for early detection and monitoring of other plant diseases through extension of its analysis parameters. Through this study, the framework can be modified and used to monitor other plant diseases.

    Time:
    14:00-14:25

    Title: The Automated Detection of Fusarium Oxysporum Sp. in Soil Samples through Mapped Methods of Image Analysis and Machine Learning

    Andrei D. Coronel
    Ateneo de Manila University, Philippines

    Biography
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    Biography

    Andrei D. Coronel
    Ateneo de Manila University, Philippines

    Dr. Andrei Coronel graduated with Bachelor of Science degree in Biology in 1999, a Master of Science degree in 2005, and a Ph.D. degree in Computer Science in 2014. He was a Research Engineer for the IIR(A-Star)/NUS in Singapore last 2008, where he contributed in the development of an ontology-based cancer database system. He involves himself with projects that intersect CS with health or environmental science, such as this recent project in UC Berkeley. He is currently the head of the Ateneo Computational Sound and Music Lab in his University, furthering his research oriented in data analytics.



    Abstract
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    Abstract

    Andrei D. Coronel
    Ateneo de Manila University, Philippines

    The export of bananas is a major area of trade in the Philippines, with Cavendish cultivars making up 50% of the export volume of this market. This type of banana, however, is susceptible to the infection of Fusarium oxysporum sp. Cubense, Tropical Race 4 (Foc TR4), the fungus that causes Fusarium Wilt. Capable of rendering wide areas of banana farms with infected plantations, Foc is a significant threat to this commerce. There is therefore a need for the early detection of Foc, especially in juvenile and asymptomatic bananas. This study has implemented an image analysis and machine learning approach in detecting Foc in magnified soil samples. Several image analysis methods were tested alongside a number of machine learning techniques towards the goal of identifying an ideal mapping of these procedures. This study not only served to improve the classification accuracy of Foc identification, but the results also acted as a guide to the development of both the soil preparation protocol and mobile microscope design. The results show that CNN, ANN, and SVM machine learning methods yield classification accuracies of 72.6%, 82.23%, and 81.12%, respectively, where SVM performed with the fastest processing time. It is conclusive that a fluorescence-based soil preparation protocol paired with at least 100x magnification and a phase contrast modality produces a viable input for a shape-recognition based image analysis technique. The study is an important step towards a multi-parameter approach in the early detection of Foc TR4 infection, thus potentially changing farmer behavior from reactive practices to preventive measures in the context of Fusarium Wilt. Similar methods can be applied in other plant diseases that make use of image analysis for early detection of diseases.

    Time:
    14:25-14:50

    Title: QCM-Based Biosensors for the Detection of Tumor Released Exosomes

    Agnese Magnani
    University of Siena, Italy

    Biography
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    Biography

    Agnese Magnani
    University of Siena, Italy

    Agnese Magnani is a Professor of Inorganic Chemistry- University of Siena,Department of Biotechnology, Chemistry and Pharmacy. Her research topics are: - Functional polymers, materials and coatings for biomedical and agriculture applications - Biosensors for biomarkersidentification and determination in body fluids - Nanocarriers for drug target delivery - Study of molecular recognition processes: protein-ligand interactions; protein and cell interaction with solid surfaces; biofilm formation - Application of IR and ToF-SIMS to materials and biological systems: thin films, SAMs and nanomaterialscharacterisation; geographical characterisation of agrifood products; development of micro-imaging methods for biological processes in tissues and cells.



    Abstract
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    Abstract

    Agnese Magnani
    University of Siena, Italy

    Biosensors cansatisfy the rapidity and accuracy diagnosis requirements in cancer biomarkers(tumorassociatedantigens) detection during early stages of the disease, thus overcoming many of the problems related to the classical diagnostic methods more expensive and often time consuming. Modernbioaffinity sensors, suchas DNA- or immunosensors, have recently demonstrated great potential for monitoring cancer-related protein markers and DNA mutations. Mass-sensitive devices like Quartz Crystal Microbalances (QCMs) are commonly used to develop bio-affinity sensors: this kind of sensor is usually made of an AT-cut quartz disk with electrodes on both sides, one of which is functionalized with a receptor selective to the target analyte. If the quartz is used as a feedback element in an electronic sinusoidal oscillator (setting in this way the oscillation frequency), in case of a bio-recognition event, the change of the mass of the quartz is proportionally converted to a frequency shift of the oscillator frequency, providing an indirect measure of the adsorbed mass with a good sensitivity (1Hz/ng for 10 MHz AT-cut quartzes). The development and the characterization of QCM biosensors for the detection of exosomes is presented. Exosomes are cell-derived vesicles that are present in many biological fluids, and that possess diagnostic potential in the oncologic field. From tests with physiological solutions and human plasma, the developed biosensors have proved to give a rapid response (within minutes) with high sensitivity and specificity against the PSMA (prostate-specific membrane antigen).

    Time:
    14:50-15:15

    Title: Removal of Lead and Mercury from Contaminated Water

    Hossein Rostami
    Philadelphia University, USA

    Biography
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    Biography

    Hossein Rostami
    Philadelphia University, USA



    Abstract
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    Abstract

    Hossein Rostami
    Philadelphia University, USA

    Over the past 15 years, about one half million sites with potential contamination have been reported to federal or state authorities. Of these, about 217,000 sites still need remediation and new contaminated sites continue to appear each year. The most common type of contaminants is metals, solvents and petroleum products. Heavy metals are present in two thirds of Department of Defense (DOD) and superfund sites and about 50% of Department of Energy (DOE) and Resource Conservation and Recovery Act (RCRA) sites. Heavy metals are the largest class of contaminates and also the most difficult to treat. Heavy metals most frequently found in the waste stream are lead, mercury, chromium, cadmium, arsenic and zinc. This work focuses on the removal of lead from aqueous solution. Lead and Mercury has long been acknowledged as a harmful environmental contaminant. Lead pollution impacts all of the body's systems. Lead exposure can adversely influence the brain, central nervous system, blood cells, and kidneys. Mercury exposure can adversely influence entire body including the brain, central nervous system, lung, skin, and kidneys. To remove lead and mercury from wastewater, several methods are utilized. Some of these methods are chemical precipitation, ion exchange adsorption, reverse osmosis and electrodialysis. All of these techniques have shortcomings, Alkali Fly Ash Permeable Reactive Barrier (AFA-PRB) is a newly developed novel material made with fly ash alkali activating solution and filler material (sand and coarse aggregates). AFA-PRB was used to remove leadand mercury from contamination water. AFA-PRB materials with high permeability were created. For reactive barrier material permeability, must be rapid, in the range of 10-2 cm/sec to 10-1 cm/sec. AFA - PRB from three ash sources with permeability of 10-1 cm/sec were produced and crushed into pelletized form. Effectiveness of the various barriers was determined by batch and column tests. Laboratory experiment indicates lead ion reduces from 1000 ppm to less than 2 ppm with 10 liters of solution and Lead ion from 10 ppm to less than 0.01 ppm. . Laboratory experiment also indicates mercury ion reduces from 1000 ppm to less than 0.5 ppm with 10 liters of solution and mercury ion from 10 ppm to less than 0.01 ppm.

    Time:
    14:50-15:15

    Title: Removal of Lead and Mercury from Contaminated Water

    Mozhgan Bahadory
    Community College of Philadelphia, USA

    Biography
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    Biography

    Mozhgan Bahadory
    Community College of Philadelphia, USA

    Dr. Bahadory is currently an assistant professor of Chemistry at Community College of Philadelphia. She received a Ph.D. in Chemistry from Drexel University, a M.S. in Environmental Engineering from Temple University as well as a M.S. and a B.S. degree in Chemistry from Azad University in Iran. Her research interests includechemistry curriculum development and laboratory experiment designs, synthesis of silver and copper nanoparticles, removal of heavy metals from contaminated water and soil using a permeable reactive barrier system and utilization of waste materials to produce new and useful engineering products. Dr. Bahadory is a Co-PI of a NSF grant which supported the development of the Biomedical Equipment Technology curriculum at Community College of Philadelphia.



    Abstract
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    Abstract

    Mozhgan Bahadory
    Community College of Philadelphia, USA

    Over the past 15 years, about one half million sites with potential contamination have been reported to federal or state authorities. Of these, about 217,000 sites still need remediation and new contaminated sites continue to appear each year. The most common type of contaminants is metals, solvents and petroleum products. Heavy metals are present in two thirds of Department of Defense (DOD) and superfund sites and about 50% of Department of Energy (DOE) and Resource Conservation and Recovery Act (RCRA) sites. Heavy metals are the largest class of contaminates and also the most difficult to treat. Heavy metals most frequently found in the waste stream are lead, mercury, chromium, cadmium, arsenic and zinc. This work focuses on the removal of lead from aqueous solution. Lead and Mercury has long been acknowledged as a harmful environmental contaminant. Lead pollution impacts all of the body's systems. Lead exposure can adversely influence the brain, central nervous system, blood cells, and kidneys. Mercury exposure can adversely influence entire body including the brain, central nervous system, lung, skin, and kidneys. To remove lead and mercury from wastewater, several methods are utilized. Some of these methods are chemical precipitation, ion exchange adsorption, reverse osmosis and electrodialysis. All of these techniques have shortcomings, Alkali Fly Ash Permeable Reactive Barrier (AFA-PRB) is a newly developed novel material made with fly ash alkali activating solution and filler material (sand and coarse aggregates). AFA-PRB was used to remove leadand mercury from contamination water. AFA-PRB materials with high permeability were created. For reactive barrier material permeability, must be rapid, in the range of 10-2 cm/sec to 10-1 cm/sec. AFA - PRB from three ash sources with permeability of 10-1 cm/sec were produced and crushed into pelletized form. Effectiveness of the various barriers was determined by batch and column tests. Laboratory experiment indicates lead ion reduces from 1000 ppm to less than 2 ppm with 10 liters of solution and Lead ion from 10 ppm to less than 0.01 ppm. . Laboratory experiment also indicates mercury ion reduces from 1000 ppm to less than 0.5 ppm with 10 liters of solution and mercury ion from 10 ppm to less than 0.01 ppm.

    Time:
    15:30-15:55

    Title: Diversity of Microorganisms in Heavy Oil Reservoirs (Russia) and their Possible Application in Meor

    Diyana Sokolova
    Russian Academy of Sciences, Russia

    Biography
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    Biography

    Diyana Sokolova
    Russian Academy of Sciences, Russia

    Dr. Diyana Sokolova, graduated from the Mendeleyev University of Chemical Technology of Russia (MUCTR) in 2001. From 2001 she has been working as a researcher at the Laboratory of Petroleum Microbiology, Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences,Moscow, Russia. She did her Ph.D., on "Formation of biosurfactants by aerobic organotrophic bacteria from oil reservoir", under the supervisor Dr. T.N. Nazina.DiyanaSokolova worked at petroleum reservoirs of Russia, China and USA. Areas of specialization: petroleum microbiology, biosurfactants, physiology of microorganisms, heavy oil,biodegradation, biotechnology, microbial enhancement for oil recovery (MEOR).



    Abstract
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    Abstract

    Diyana Sokolova
    Russian Academy of Sciences, Russia

    Geological reserves of heavy and high-viscosity oil in Russia reach 6–7 billion tons and exceed residual reserves of light conventional oil. The goal of the present work was to study the diversity and activity of microorganisms from low-temperature heavy oil reservoirs (Russia) and their biotechnological potential for development of the microbiological method for enhanced recovery of heavy oil. Microorganisms are able to produce diverse oil-displacing agents (organic and mineral acids, biosurfactants, solvents, biopolymers, gases), which can be in technologies for microbial enhancement of oil recovery (MEOR). The functional and phylogenetic diversity of microorganisms at five heavy oil deposits (Russia) was studied. The oilfieldsexploited without water-flooding, were shown to harbor scant microbial communities, while microbial numbers in the water-flooded strata was high. Methanogens of the genera Methanothrix,Methanobacterium, and Methanoregulawere revealed by high-throughput sequencing of 16S rRNA genes and DGGE analysis of mcrAgenes. Aerobic bacteria of the genera Rhodococcus, Pseudomonas, Gordonia, Cellulomonas, etc., capable of biosurfactant production, were isolated. Fermentative enrichments producing volatile organic acids (acetic, propionic, and butyric) from sugar-containing substrates were obtained. These acids dissolved the carbonates of oil-bearing rock efficiently. The major microbial groups of interest for development of MEOR technology are aerobic oil-oxidizing bacteria, anaerobic fermenting bacteria, and methanogenic prokaryotes. Core-flooding experiments with injection culture liquid of aerobic bacteria Gordoniaamicalis6-1 and Rhodococcuserythropolis KS22 resulted in recovery of 17.6% of additional oil from the models because of biosurfactants and biomass production. Application of starch and culture liquid of fermenting enrichment capable of producing low fatty acids resulted in recovery of 13.4% of additional oil from the models.Selection of the most efficient microbial technology for enhanced recovery of heavy oil from terrigenous and carbonate strata is discussed.

    Time:
    15:55-16:20

    Title: Assessing the Microbiome Dynamics in Three Photo-Bioreactors Established for Coking Wastewater Treatment: An Orchestration between Microalgae and Bacterial Communities

    Mariam Hassan
    Cairo University, Egypt

    Biography
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    Biography

    Mariam Hassan
    Cairo University, Egypt

    Mariam Hassan is an Assistant Lecturer in Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University. She teaches the practical courses to the undergraduate and graduate students. She is working on her Ph.D. project on biomonitoring water microbiome(s) involved in biological wastewater treatment processes. Areas of specialization: microbiology, biotechnology, biodegradation, biosurfactants, biodiversity, bioinformatics and high throughput data analysis.



    Abstract
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    Abstract

    Mariam Hassan
    Cairo University, Egypt

    The investigation of microbial community structures is a significant way to understand biodegradation capacities in biological wastewater treatment processes. Photo-bioreactors A, B and C received real coking-wastewater as influent with COD 776 ± 56, 1229 ± 85 and 2033±27 mg/l, respectively. In phase-1 phenol was added to the influent, while dichlorophenol was added in combination with phenol in phase-2. Treatment efficiency of algal-bacterial systems was biomonitored using different bioassays (phytotoxicity, Artemia toxicity, cytotoxicity, algal-bacterial ratio and settleability). COD removal %, phenol and dichlorophenol concentrations were also monitored. All systems efficiently detoxified the influents in phase-1. In phase-2, Systems B and C failed to detoxify the influents. Illumina-sequencing generated 2119749 effective sequences of 16S-rRNA gene from 21 samples collected from different influents and effluents. The number of observed species was significantly lower in effluent samples than influent samples, as some taxa dominated in photo-bioreactors and contributed to the systems performance. Significant difference in the microbial diversity between influent and effluent samples was detected. Proteobacteria (78 %), Firmicutes (12 %), Bacteroidetes (5 %) and Deferribacteres (2 %) were the dominant phyla in influent samples. While in effluent samples Proteobacteria (68 %) and Bacteroidetes (25 %) dominated. Failure in treatment process in systems B and C at phase-2 was accompanied with significant difference in the microbial diversity. Significant relative abundance of anaerobic bacteria from Deferribacteraceae and Peptococcaceae families in influent samples conformed to the nature of coking-wastewater. The co-culture of microalgae shifted the microbiome and promoted the activity of genera affiliated to Chitinophagaceae, Pseudomonadaceae and Xanthomonadaceae families, which dominated in effluent samples. These bacteria are known for their catabolic diversity that enables xenobiotic degradation. The superiority of algal-bacterial systems for coking-wastewater treatment was confirmed as co-culture of microalgae eradicated pathogenic bacteria such as Arcobacter and Legionella genera in the treated effluent.

    Time:
    16:20-16:45

    Title: Increased Concentration of Diesel in Soil has Varying Impacts on Different Soil Biological Activities

    Chukwudi Onwosi
    University of Nigeria, Nigeria

    Biography
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    Biography

    Chukwudi Onwosi
    University of Nigeria, Nigeria

    The resultant effects of the persistent contamination of the soil environment by organic pollutants are known to be deleterious to soil components and by extension, humans. Therefore, bioremediation monitoring is pertinent in ensuring the effective and efficient restoration of soil activities. In this study, soils polluted with varying concentrations of diesel (IC 1, IC 2, IC 3) at initial TPH concentrations of 14785.84 mg/kg, 23859.52 mg/kg and 42134.96 mg/kg, respectively, were bioremediated using rice husk as biostimulant. Different soil biological parameters namely soil enzyme activities (catalase and -glucosidase), soil microbial biomass carbon (MBC), nitrogen (MBN) and phosphorus (MBP), soil microbial respiration as well as the soil phytotoxicity were used to monitor the bioremediation process. At the end of 56-day study, the degradation rate for IC 1, IC 2 and IC 3 were recorded at 99.1%, 98% and 97.6% respectively. The 1st, 2nd and nth-order kinetic equations were used in determining the efficiency of the treatment for the increasing concentrations of diesel polluted soils. The results of 1st order kinetics for IC 1 (k= 0.6745 d-1, R2= 0.9388); IC 2 (k= 0.5738 d-1, R2= 0.9287); IC 3 (k= 0.6058 d-1, R2= 0.9626); 2nd order kinetics for IC 1 (k= 8.748×10-5 d-1, R2= 0.8594); IC 2 (k= 4.301×10-5 d-1, R2= 0.8457); IC 3 (k= 3.046×10-5 d-1, R2= 0.9839); nth order kinetics for IC 1 (R2= 0.9492, k= 4.472 d-1, n= 0.7919); IC 2 (R2= 0.9394, k= 6.239 d-1, n= 0.7517); IC 3 (R2= 0.9882, k= 0.0028 d-1, n= 0.3082). From the results obtained, all biological activities for IC 1 except MBN were most responsive to the rice husk treatment than those of the IC 2 and IC 3. Improved plant growth was also observed in IC 1 and IC 2, as compared to IC 3, towards the end of the bioremediation study. These outcomes showed that the use of biological parameters is indispensible in monitoring the efficacy of a bioremediation process on contaminated soil.



    Abstract
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    Abstract

    Chukwudi Onwosi
    University of Nigeria, Nigeria

    Time:
    16:45-17:10

    Title: Effect of Clarifying Agents on the Volatile Composition of Mead

    Leticia M. Estevinho
    Instituto Politecnico de Braganca, Portugal.

    Biography
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    Biography

    Leticia M. Estevinho
    Instituto Politecnico de Braganca, Portugal.

    Leticia M. Estevinho received the Zootechnical Engineering degree in 1985, the MS Degree in Biotechnology in 1989 and the PhD Degree in Science in 1995. In 2009 passed with merit the proofs of “Agregacao”. Leticia M. Estevinho published more than 100 articles in indexed international journals, wrote several book chapters and presented more 160 oral and written communications. Leticia Estevinho is the Head of the Microbiology Laboratory of Escola Superior Agraria de Braganca since 1986 and has been the principal investigator of more than 30 financed research projects.



    Abstract
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    Abstract

    Leticia M. Estevinho
    Instituto Politecnico de Braganca, Portugal.

    Mead is an ancient alcoholic beverage containing between 8% and 18% alcohol by volume. It is obtained by fermentation of honey-wort though a complex process demanding both long-term fermentation and maturation. In wine production different procedures are applied for organoleptic properties’ stabilization and improvement, among which clarification. However, studies regarding those procedures in the context of mead fermentation are practically non-existent. This study aimed to assess the effect of several clarifying agents (i. casein, ii. gelatin, iii. silica, iv. bentonite, v. tannins and vi. bentonite + gelatine + egg yolk) on the volatile composition of mead. The volatile compounds were determined by gas chromatography fitted with a flame ionisation detector (FID) and by gas chromatography-mass spectrometry (GC-MS). Thirty-six volatile compounds were identified, among which 42.50% belong to the group of alcohols, 40.40% were carbonyl compounds, 14.40% were acetates and 1.8% were esters. Volatile compounds’ concentration differed according to the concentration and type of fining agent used. Significant differences (p < 0.05) were found in ten volatile compounds independently of the type of treatment used. Highest volatile compounds’ concentration, mainly esters, lactones, terpenes and norisopenoids, were observed in meads clarified with silica; the lowest values were obtained for samples in which tanines were used. From the identified compounds, eleven had major impact on meads’ aroma, with OAV>1. These results contribute to select the fining agent to be used in mead fermentation and, to a later extent, to improve the sensorial characteristics of this beverage.

    Time:
    17:10-17:35

    Title: Potential of Indigenous Lactococci as Starter Cultures in Dairy Industry

    Mirjana Bojanic Rasovic
    University of Montenegro, Montenegro.

    Biography
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    Biography

    Mirjana Bojanic Rasovic
    University of Montenegro, Montenegro.

    Dr. Mirjana Bojanic Rasovic began to work in 1994 at Biotechnical Institute, now Biotecnical faculty in Podgorica. From 2012, she is a senior research associate in the field of Animal hygiene and Preventive Diseases and Microbiology. For two terms (2003 to 2007), she was president of the Society of Microbiologists of Montenegro for which time she organized several scientific meetings and lectures. She has participated in the work of a number of domestic and foreign scientific and professional conferences. As author or co-author, about 80 scientific and professional papers have been published. She was the head of national projects "Influence of zoohygienic conditions on the appearance of mastitis in high dairy cows", 2001-2003., "Examination of the spread of bovine infections with Mycoplasma bovis in the Montenegro",Isolation and characterization of indigenous lactic acid bacteria for the production of specific cheeses in Montenegro", (2009-2012). Dr Mirjana Bojanic Rasovic is Member of following committees: The Veterinary Chamber of Montenegro, Commission for Accreditation of the Accreditation Body of Montenegro, Technical Committee for Laboratories of the Accreditation Body of Montenegro, Sectoral Commission for Agriculture, Food Processing, Committee for Agriculture and Forestry of the Montenegrin Academy of Science and Arts, Commission of the Ministry of Agriculture and Rural Development for professional exam for graduate veterinarians.



    Abstract
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    Abstract

    Mirjana Bojanic Rasovic
    University of Montenegro, Montenegro.

    The diversity of lactic acid bacteria in traditional dairy products represents great potential in biotechnology. In order to standardize indigenous products, the basic requirement is the application of the determined indigenous lactic acid bacteria as starter cultures affecting their specific characteristics by performing fermentation and influencing the ripening process. Starter microorganisms produce lactic acid, which is very important during the coagulation and texturizing of the curd cheese. Production of volatile compounds (e.g. diacetyl and acetaldehyde) contribute to the flavor of these dairy products. Starter culture may posses a proteolytic and lipolytic activity that may be desirable, especially during the maturation of some types of cheese. They also produce bacteriocins, which prevents the growth of pathogens and many spoilage microorganisms. In the process od fermentation of cheese usually participate bacteria of the genus Lactococcus-Lc.lactis ssp. lactis, Lc. lactis ssp. cremoris and homofermentative lactobacilli. Lactococcus lactis species is one of the most important of lactic acid bacteria that are used in the dairy industry. The major functions of this species in dairy fermentation are the production of lactic acid from lactose, hydrolysis of casein and citric acid fermentation. Their metabolic end products and enzymes directly or indirectly have significant influence in determining the texture and flavour of the final products. The utilization of lactococci isolated from indigenous fermented milk products would lead to potential starter cultures with the necessary properties for typical local products that are well accepted by the local population. Also, the use of such starter cultures would allow the production of cheese with designated geographical origin.

    Time:
    17:35-18:00

    Title: Production and Evaluation of Protease Enzyme (B.subtilis strain) for Eco-friendly Leather Processing Empowerment in Ethiopian Tanneries

    Biruk Abate
    Ku Lueven Engineering College, Belgium.

    Biography
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    Biography

    Biruk Abate
    Ku Lueven Engineering College, Belgium.

    Biruk Abate Fenta is Lecturer and researcher at Bahir Dar Institute of Technology, Bahir Dar University in the Faculty of Chemical and Food Engineering and Biotechnology Research Institute. He received a B.S. degree in Chemical Engineering from Bahir Dar Institute of Technology, Bahir Dar University, Ethiopia and the Master of Technology in Chemical Engineering from the Addis Ababa Institute of Technology, Addis Ababa University, Ethiopia. He has been active in the area of Postharvest and Energy technologies, water quality and environment, chemicals and Pharmaceuticals researches in addition to his regular work of lecturing undergraduate students and mentoring postgraduate students during their thesis work. His national experience includes consulting feasibility project works of household/small scale, medium and large scale industries across his country. Currently he is an international PhD student in Ku Leuven Engineering Technology, Leuven, Belgium



    Abstract
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    Abstract

    Biruk Abate
    Ku Lueven Engineering College, Belgium.

    Across the world the tanning industries are facing a high challenge pressure from strict legislation articulated for the purpose of protecting and preserving the environment. In Ethiopia leather processing industry is considered from the directions of economic activity and environment polluting concern. In order to operate it within the environmentally compatible situations and sustain the agriculture development led industrialization policy and the ongoing development strategy of the tanning industry in Ethiopia should come out that can mitigate the adverse effect of the conventional leather processing methods particularly that of lime-sulphide dehairing in beam house operation. This study was focused on dehairing enzyme production as green technology alternative for the existed conventional unhairing practice. The B.subtilis strain was produced from microorganism from beam house waste from the local leather processing areas, sub-cultured and characterized for its growth and dehairing protease production in terms of pH, temperature, incubation time and growth and production media composition. The combined effects of pH and temperature on protease production also investigated and they were found to have high interactive effect. After the culture conditions for production were clearly studied the alkaline protease was produced in a laboratory scale fermenter containing ingredients with required concentration of glucose , peptone,MgSO4.7 H2O, KH2PO4 and FeSO4.7H2O and it was maintained at a temperature about 37°C for 24 to 48 hoursand trembled uncontrollably in the incubator operated close to 140rpm. After completion of fermentation time all fermented sauce base of the broth was separated by centrifuge rotated at 10,000 rpm at 4°C for 15-18 min and the clear liquid part was taken for crude enzyme preparation. From this clear supernatant liquid part the concentration of protease enzyme produced in the production medium was analyzed at best conditions near to PH 8.5, temperature 37°C and incubation time of 24 hours and closer to the 96 hrs operations maximum amunt of enzyme was yielded. The final protease product was recovered, partially purified and stabilized by primary downstream processing such as crude enzyme formulation, ammonium sulphate precipitation. The use of the protease product of this technique on sheep skins resulted in a highly promising hair removal efficiency that can really compete with lime-sulphide chemical unhairing process.

  • Keynote Speaker

    Time:
    09:30-10:15

    Title

    Title: Polysaccharide Bacterial Toxins as Anticancer Agents

    Roger A Laine
    Louisiana State University, USA
    Biography
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    Biography

    Professor Roger Laine, B.A., University of Minnesota, PhD, Rice University, Houston, Texas (Professor Alan D. Elbein), postdoctorals with Prof. Charles C Sweeley at Michigan State University, and Prof. Sen-itiroh Hakomori, University of Washington. He was Assistant and Associate Professor, University of Kentucky-Medical in Lexington, Kentucky, and then Professor and Chair of the Department of Biochemistry, Louisiana State University, currently Professor of Biochemistry and Chemistry. He was Chief Scientist, Glycomed, Inc., San Francisco, and Founder of biotechnology companies Anomeric, Inc., TumorEnd, LLC (www.tumorend.com), Citrazone, LLC, Enzomeric, LLC, and partner in Glycon, LLC, and has authored 150 publications, and 28 patents.



    Abstract
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    Abstract


    Keynote Speaker

    Time:
    10:30-11:15

    Title

    Title: Tumor Extracellular Matrix Microenvironment, Role of Macrophages

    Henry Lopez
    Raptide Biosciences, USA
    Biography
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    Biography

    Henry Lopez is a founder, CEO/CSO of MuriGenics, Inc. which is a preclinical contract research organization working with national and international companies, located in Vallejo, California. Prior to this he worked with many Northern California biotechnology companies such as Pfizer, Parke-Davis, GeneNetworks, Glycomed, Xoma and Cetus. In 2012 he founded Riptide Bioscience (Senior Vice President) a synthetic peptide company focusing on immunomodulation and antimicrobials. Have licensed out one peptide to Pharma for treating pancreatic cancer- working partner has been the NCI for the last 4 years. He received PhD from the University College London –Royal Free Hospital



    Abstract
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    Abstract


    Sessions:
    Pharmaceutical Biotechnology & Biotechnology in Healthcare & Biotechnology for Genetic disorders & Immunology and Therapeutic Antibodies

    Time:
    11:15-11:40

    Title: The Casein Kinase 2 (CK2) and PIm Kinase as a Target for Anticancer Therapy

    Maria Bretner
    Warsaw University of Technology, Poland

    Biography
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    Biography

    Maria Bretner
    Warsaw University of Technology, Poland

    Maria Bretner received M.S. degree in chemistry from the Faculty of Chemistry , University of Warsaw in 1974 and Ph.D in biological sciences from the Institute of Biochemistry and Biophysicsin 1997.She completed postdoctoral studies at the University of Maryland Baltimore County, USA. (1997-1999). She worked at the IBB PAS developing the methods of new inhibitors synthesis of Thymidylate Synthase, HIV RT andHCV helicase. Since 2007 she is working at the Warsaw University of Technology,Faculty of Chemistry .Research areas include chemistry andenzymology, biocatalysis, biochemistry of the cancer processes. Co-author of 69 scientific papers.



    Abstract
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    Abstract

    Maria Bretner
    Warsaw University of Technology, Poland

    Protein kinases CK2 and PIM belong to serine/threonine kinases that are involved in the regulation of number of signalling pathways. They are upregulated in multiple cancers, including lymphoma, leukemia, breast, prostate, head and neck cancers and act as repressors of apoptosis, contributing to chemoresistance. Over 30 drugs, that target kinases, have been approved for clinical use over the past decade, and hundreds more are undergoing clinical trials, among them are: specific inhibitor of CK2-CX4945 and few specific inhibitors of PIM family e.g. AZD1208, CX-4595, SGI-9481 or LGB321. But their efficiency is not satisfactory so the increasing attention has been recently focused on the significant role of dual kinase inhibitors. Until now a few examples of the benzimidazole derivatives, which affect the activity of both kinases, CK2 and PIM1 have been known. We designed and synthesized novel 4,5,6,7- tetrabromo-1H-benzimidazole (TBBi) and 4,5,6,7- tetrabromo-1H-benzotriazole (TBBt) derivatives with alkylamine substituents. To test the inhibitory properties recombinant human kinase CK2α, CK2 holoenzyme, and kinase PIM1 was obtained in E. coli bacterial expression system. The most promising compound, the 3- (4,5,6,7-tetrabromo-2 methyl-1H-benzimidazol-1-yl) propan-1-amine (14b) inhibited the activity of CK2 with an IC50 of 0.35 micromolar and PIM1 0.15 micromolar. Furthermore, the influence of new active dual inhibitors on the cell viability was evaluated and EC50 determined for 14b with the use of CCRF-CEM, MCF-7 and PC3 cell lines were in the range of 2-4 micromolar. This work was supported by an NSC Poland grant 2014/13/B/NZ7/02273 and by WUT.

    Time:
    11:40-12:05

    Title: Native Cell Membrane Nanoparticles System for Membrane Proteins

    Youzhong Guo
    Virginia Commonwealth University, USA

    Biography
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    Biography

    Youzhong Guo
    Virginia Commonwealth University, USA

    Youzhong Guo was born in Xiangcheng, Henan, China, in 1974. He received the B.S. degree in biology from Henan Normal University, Xinxiang, Henan, China, in 1997. He received the Ph.D. degree in pharmacy/structural biology from the University of Texas at Austin, Austin, TX, U.S.A., in 2010. From 2010 to 2016, he worked with Dr. Wayne A. Hendrickson as a postdoc in Columbia University, New York, NY, U.S.A. In 2016, he joined the Department of Medicinal Chemistry, Virginia Commonwealth University, Richmond, VA, U.S.A. as an Assistant Professor. His current research interests include membrane protein structural biology and structure-based drug discovery.



    Abstract
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    Abstract

    Youzhong Guo
    Virginia Commonwealth University, USA

    We devised a native cell membrane nanoparticles system, which we applied in a single-particle cryo-EM study of the multidrug exporter AcrB. Lipid-AcrB nanoparticles were prepared directly from membranes without any use of detergents. A 3D reconstruction in C1 symmetry achieved a final density map at 3.2 Å resolution, an atomic model of quasi-C3-symmetric AcrBwas fitted to this map, and the residual density revealed many ordered lipid molecules. Most remarkably, a central cavity between the three transmembrane domains contains a 24-lipid patch of well-ordered bilayer structure. Inner leaflet lipid chains pack in a hexagonal array like that in phosphatidylethanolamine crystal structures, whereas the outer leaflet has highly irregular packing. Protein side chains interact with both leaflets and participate in the hexagonal pattern. The AcrB export mechanism requires reorganization of the lipid bilayer structure. This system should be broadly applicable for membrane protein structural biology and structure-based drug discovery and development.

    Time:
    12:05-12:30

    Title: Small Molecular Weight Peptides as Potential Therapeutics: Anticancer and Antimicrobial Effects of an Analogue to a Viral Protein

    Taghrid Istivan
    RMIT University, Australia

    Biography
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    Biography

    Taghrid Istivan
    RMIT University, Australia

    Dr. Istivan is a senior academic at RMIT University, Melbourne, Australia within the Biotechnology Discipline. Her research interests are in bacterial virulence, therapies and drug development. She was awarded a PhD in molecular microbiology in 2005 and has been affiliated with RMIT University as a researcher and a lecturer for 15 years. She has also supervised several PhD and Masters research projects within her research team and authored and coauthored research articles and book chapters in the fields of microbiology and novel therapeutics. She is currently the Biosciences senior program manager (Teaching and Learning) within the School of Science.



    Abstract
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    Abstract

    Taghrid Istivan
    RMIT University, Australia

    The resonant recognition model (RRM) can be employed to de novo design small molecular weight peptide analogues to known anticancer proteins, like viral proteins, interleukins, and tumor necrosis factor (TNFα). The biological effects of RRM-MV, an 18 aabioactive peptide analogue to myxoma virus NM-T5protein (AAC55050, 483 aa), were investigated on mammalian cell cultures and selected bacterial pathogens using qualitative and quantitative cell survival methods. Human apoptosis protein arrays were used to detect the levels of pro-apoptotic and anti-apoptotic proteins in treated cancer cells versus non treated cells. The small molecular weight peptide (2.3 kDa) produced cytotoxic effects on cancer cell lines including mouse melanoma (B16), human melanoma (MM96L), squamous cell carcinoma (COLO16), prostate cancer (PC3) and breast cancer (MCF7). Yet, no cytotoxic effects were detected on human red blood cells, skin fibroblasts and other normal mammalian cell lines. Furthermore, RRM-MV exhibited a bacteriostatic effect on Gram positive bacteria such as Staphylococcus spp. Furthermore, in treated cancer cells and prior to necrosis stage, the peptide was located inside cytoplasmic components predicted to be glycoproteins, and it also expressed different levels of binding specificity to glycans like Sialy Lewis X, gangliosides and mannoses when glycan arrays were performed to evaluate the binding affinity of RRM-MV. The data from this study indicated that RRM designed peptides such as RRM-MV have a potential to be developed as effective therapeutics, mostly for their ability to penetrate cellular membranes, and to interfere with specific cellular biological functions.

    Time:
    12:30-12:55

    Title: The Effect of Etoposide Drug on Chromosomal Changes and Mitotic Index in Mouse Bone Marrow Cells

    AbdulRahman A. I. Alyahya
    Shaqra University, Saudi Arabia

    Biography
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    Biography

    AbdulRahman A. I. Alyahya
    Shaqra University, Saudi Arabia

    Dr. Abdul Rahman A. I. Alyahya is working in the Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, KSA. He is a cell Biologist scientist since 2005. He has strong research and teaching experience in cell Biology, toxicology, animal cell culture, Anticancer Drugs and pharmaceutical biotechnology. He obtained his PhD from King Saud University in Biology. He joined ShaqraUniversity in 2009 as assistant professor and promoted to associate professor. Since 2009, he has secured many national grants for many research projects. He has published 31 refereed Journal papers, 12 conference presentations, 3 industrial reports, two conference proceedings. Formerly he was the dean College of science and currently he is a Vice Rector of Shaqra University for services and consultancy affairs. He has research interest in Cell biology, Toxicology, Animal Cell Culture, Pharmaceutical Biotechnology and Environmental Biotechnology.



    Abstract
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    Abstract

    AbdulRahman A. I. Alyahya
    Shaqra University, Saudi Arabia

    Etoposide is one of the important chemical drugs commonly used worldwide in the treatment of many cancers and it is classified as an anti metabolic drug as it disrupt the process of multiplication of DNA in cancer cells by inhibiting DNA topoisomerase II causing the death of cancer cells. We select short-term tests in the current study, because it is a quick and accurate indicator to study the effect of chemicals and drugs on biological systems. As there are changes can determined accurately by cytological examination. Laboratory male mice of pure SWR / J were exposed to three types of concentrations (20, 40, 60 mg / kg) of Etoposide at different times to study its effect on the index rate of indirect cell division, and the extent of chromosomal abnormalities that can be caused. Treatment with Etoposide drug led to significant decrease in the index rate of cell division and significant increase of chromosomal abnormalities, and it correlates directly with concentration and inversely with the passage of time after treatment.

    Time:
    14:00-14:25

    Title: Effect of Porphyrinoids on the Infectivity of Nosema spp.Microsporidia

    Mariusz Trytek
    Maria Curie-Skłodowska University, Poland

    Biography
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    Biography

    Mariusz Trytek
    Maria Curie-Skłodowska University, Poland

    Dr. Mariusz Trytek is Assistant Professor at the Department of Industrial Microbiology, the Faculty of Biology and Biotechnology of Maria Curie-Skłodowska University in Lublin, Poland. He received his M.S. degree in organic chemistry in 2000 and earneda PhD in biological sciences in 2007 from MCSU, with which he has been affiliated as a researcher and a lecturer for 16 years now. Dr.Trytek has supervised several bachelor and master theses. His current research interests include biomimetic catalysis with porphyrins, biotransformation of organic compounds, and biomedical applications of porphyrinoid compounds. He has co-authored over30 publications (including research papers, reviews, and book chapters), and four patents. He is currently the principal investigatorin the national project "The biological activity of porphyrinoids and mechanisms of their action against intracellular bee pathogens of the genus Nosema", 2016–2019.



    Abstract
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    Abstract

    Mariusz Trytek
    Maria Curie-Skłodowska University, Poland

    Pathogenic fungi from the phylum Microsporidia are intracellular parasites found in both invertebrates and vertebrates. Nosema apis and N.ceranae are particularly devastating to honeybees (Apis mellifera carnica) as they complete their life cycle in the insects’ intestinal tract, causing total colony collapse. In the present work, protoporphyrin amide derivatives [PPIX (Lys)2, PPIX(Asp)2, PPIX(Lys-Lys)2] and porphyrins H2TTMePP and H2TmePyP were studied for their bioactivity against microsporidia isolated from dead honeybees. The microsporidia were treated in vitro with aporphyrin before they were usedto infect the honeybees. The Nosema spp. spores were isolated from winter beehive debris, purified, and incubated in a 0.5% sucrose solution (2 × 10-7 spores/mL) with one of the five porphyrins (100 μM)in the dark on a shaker at 30°Cfor 24 h. A control experiment without aporphyrin was performed simultaneously. Next, the spores were centrifuged, washed with sterile solutions of 0.9% NaCl and 0.5% sucrose to remove porphyrins, and used to infect honeybees. Differences in the infectivity of pretreated and untreated spores were determined in vivo by measuring the number of spores developed in living honeybees on the 7th, 12th and 20th day of the cage test experiment. Microsporidia preincubated with the porphyrins showed a lower infectivity. The largest differences were observed on days 12 and 20. The level of infection in bees infected with porphyrin-treated spores was 2-fold lower than in the control, and in the case of PP(Lys-Lys)2, even 3.3-fold lower. At the same time, lower bee mortality (up to 50 %) was observed compared to the control group. Morphological changes and deformations of the cell wall of the microsporidia treated with porphyrins were observed by light and scanning electron microscopy (SEM). Inactivation of Nosema spp. spores with porphyrins reduces their ability to infect honeybees and develop in their intestines, thus diminishing bee mortality. This work was financially supported by the National Science Centre, Poland (2015/17/B/NZ9/03607).

    Time:
    14:25-14:50

    Title: Curcumin Reduces Antioxidant Activity Following Spinal Cord Injury in Rat Model

    Ahmed Abdellatif
    The American University in Cairo, Egypt

    Biography
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    Biography

    Ahmed Abdellatif
    The American University in Cairo, Egypt

    Ahmed Abdellatif is an assistant professor of biology at the School of Sciences and Engineering, The American University in Cairo, Egypt. He received an MSc and a PhD from the University of Louisville, School of Medicine in Anatomical Sciences and Neurobiology. He also earned an MBBCH and a Master of Science in anatomy and embryology from Alexandria University School of Medicine. Before joining AUC, he was involved in curriculum design for medical education and taught medical and graduate level courses in human gross anatomy, neuroscience. Dr. Abdellatif has been interested in drug delivery and the development of cost effective treatments using natural herbal extracts for various applications.



    Abstract
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    Abstract

    Ahmed Abdellatif
    The American University in Cairo, Egypt

    Spinal cord injury is a debilitating disability. Oxidative damage and inflammation are two hallmarks of the secondary spinal cord injury. The objective of this study was to evaluate the potential of Curcumin (a polyphenolic compound extracted from the rhizome of Curcuma longa that has been known to possess antioxidant and anti-inflammatory properties) as an antioxidant and anti-inflammatory agent following spinal cord injury in rats, and to compare its therapeutic effects following local application directly to the injury versus its oral dietary supplementation in a spinal cord hemisection model at T9-T10. Female Sprague Dawley rats were randomized into a control, injury, and treatment groups of local single dose of Curcuma longa extract immediately on the injury site and a Dietary supplement group. Crude Curcumin was added to the animals’ feed (10% of daily feed) one week before and week after injury. Oxidative stress parameters were Malondialdehyde (MDA) and total antioxidant capacity (TAC). Expression of tumor necrosis factor alpha (TNF α) and interleukin 6 (IL-6) was detected using Enzyme Linked Immunosorbent Assay (ELISA). Our results show that at 7 days, although Dietary supplement was effective in increasing TAC levels and lowering TNF αexpression levels, it did not affect MDA levels. Local treatment regimen has shown to be more effective on all four parameters. Our results demonstrate that local Curcumin application directly on the injury site might be more efficacious in alleviating oxidative damage and reducing inflammation following spinal cord injury.

    Time:
    14:50-15:15

    Title: Genomic Modification by Ocimum canum Against Lead-Induced Chromosom -Aberration and its Effect on Antioxidant Enzymes

    Oladimeji Tugbobo
    Federal Polytechnic, Nigeria

    Biography
    χ

    Biography

    Oladimeji Tugbobo
    Federal Polytechnic, Nigeria



    Abstract
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    Abstract

    Oladimeji Tugbobo
    Federal Polytechnic, Nigeria

    Anticlastogenic potential of Ocimum canum (Black leaf) extract was studied in bone marrow cells of mice using micronucleus assay. 200mg/kg of Ocimum canum aqueous extract was administered as dietary supplement for 30-days. The mice were divided into three groups A, B and C. Animals in group A were fed with distilled water, B were treated with 2.5mg/kg lead acetate while group C were fed with 200mg/kg Ocimum canum aqueous extract and 2.5mg/kg lead acetate simultaneously. After 30-days, mice were sacrificed and chromosome preparations were made from bone marrow according to colchicines hypotonic-fixation air drying Giemsa schedule. The cytogenic end-point observed was chromosomal aberration which increased significantly (P<0.05) in group B animals treated with lead acetate only. However, the chromosomal aberration was significantly (P<0.05) reduced by the extract fed to animals in group C. In addition, the effect of the extract on the defensive antioxidant enzymes of the test animals was also assessed. The results indicate synergistic effect of the extract on the antioxidant enzymes in the liver tissues. Hence, the results of this study suggest viable anticlastogenic and antioxidant potentials of Ocimum canum extract which could protect against lead-induced chromosomal aberration and as well enhance activities of antioxidant enzymes.

    Time:
    15:30-15:55

    Title: Molecular Characterization , Genetic Diversity and Similarities of Cladosporium species as Revealed by: Internal Transcribed Spacer – Polymerase Chain Reaction (ITS-PCR)

    Mohammed Alhussaini
    Shaqra University, Saudi Arabia

    Biography
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    Biography

    Mohammed Alhussaini
    Shaqra University, Saudi Arabia



    Abstract
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    Abstract

    Mohammed Alhussaini
    Shaqra University, Saudi Arabia

    The current investigation compared genetic diversities, and genetic similarities within and among Cladosporium species populations using PCR-based markers. Nuclear ribosomal DNA internal transcribed spacers have been used successfully to analyze intraspecific and interspecific relationships in various fungi. In the current study, we have used the internal transcribed spacer (ITS) to aid compare the ITS in length and restriction patterns. The internal transcribed spacer (ITS) was amplified using polymerase chain reaction combining primers ITS4 and ITS5. PCR products were digested with three restriction enzymes and separated by agarose electrophoresis. Restriction patterns generated by CfoI and MspI and RsaI were unique for most species assayed. Clear results were obtained by using ITS-PCR in the present study. The results were consistent with those based on biological characteristics and morphological features. The ITS-PCR fingerprinting methods presented here led to clear differentiation of the isolates at the species level. Fingerprinting profiles generated readily discriminated between each of the 6 species. Cluster analysis further supported this observation and clusters corresponding to each species are readily identified in the dendrograms.

    Time:
    15:55-16:20

    Title: Fine Tuning of the Silver Bullet; Epigenetic Manipulation of Immune Checkpoints in Breast Cancer

    Hend M. El Tayebi
    German University in Cairo, Egypt

    Biography
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    Biography

    Hend M. El Tayebi
    German University in Cairo, Egypt

    Dr. EL Tayebi is an assistant professor of Genetics and Genetic Engineering and she is heading the “Genetic Pharmacology Research Group” in the faculty of Pharmacy and Biotechnology, German University in Cairo (GUC), Egypt. El Tayebi received her bachelor degree with highly honored excellence from faculty of Pharmacy and Biotechnology, GUC, in 2008. In 2009, she has been appointed as assistant lecturer of molecular pathology and pathophysiology at the GUC. And in 2014, she started teaching Cell Biology and Genetic Engineering as an assistant professor. She pursued her M.Sc. and PhD studies in the field of Molecular Pathology in the period from 2008-2012. She received her PhD degree with “Summa cum laude” from the GUC in 2012. After PhD, El Tayebi worked as a senior scientist and as a team leader of hepatocellular carcinoma (HCC) group under the umbrella of the MPRG. During her postdoctoral period (2012-2015), she focused on developing the research skills of her HCC team as well as herself by developing new lab techniques, writing proposals and earning research funds.



    Abstract
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    Abstract

    Hend M. El Tayebi
    German University in Cairo, Egypt

    Programmed cell death protein-1 (PD-1) is an immune checkpoint receptor that prevents overstimulation of immune responses with an essential role in cancer immunity. Blockade of immune checkpoints especially PD-1/PD-L1 became a principle approach in cancer therapy. Different subtypes of Breast Cancer (BC) have different biological behaviors and distinct gene expression profiles. Moreover, microRNAs and Long-noncoding RNAs (LncRNAs) have gained wide spread attention recently as a critical key players in carcinogenesis but their underlying mechanisms remain limited. Our previous data revealed that overexpression of LncRNA; X inactive –specific transcript (XIST); negatively regulates PDL-1 in BC. Our work aims to investigate the impact of microRNAs and LncRNAs on PD-L1 in MDA-MB-231 cells, in addition to analyzing the differential expression of PD-L1 and non coding RNAs in different BC subtypes. Our data sheds the light on the differential expression of miR-182, MALAT-1 and XIST with a significant correlation to PD-L1 in TNBC and IDC. The data suggests a crucial role for these genes in the extent of evasion of cancer immunity in different BC subtypes.

    Time:
    16:20-16:45

    Title: A Comprehensive Dynamic Model of Gut Microbiome

    Jun Geng
    Chalmers University of Technology, Sweden

    Biography
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    Biography

    Jun Geng
    Chalmers University of Technology, Sweden

    She is a postdoc in Chalmers University of Technology, her major project is investigate dynamic modeling of gut microbiota and how its interaction relationship with host, contributing to host health. Before coming to Chalmers, she graduated from Shanghai Jiao Tong University and get her PhD degree there. During her PhD study, she had been in Purdue University for two years as a visiting Scholar.



    Abstract
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    Abstract

    Jun Geng
    Chalmers University of Technology, Sweden

    Human microbiome, predominately residing in the large intestine constitutes a vastly complex ecosystem encompassing trillions of bacterial cells belonging to hundreds of species/taxa, have recently been revitalized as its extraordinary symbiosis relationship with host during their coevolution history. Long period ecological stability of this complex system with balanced composition is extremely critical to maintain host well-beings by exerting beneficial influence on host immune system development, energy harvest and reproductive physiology mediation. Advances in metagenomics and 16S pyrosequencing promote enormous efforts in uncovering the association between distinct microbial composition and specific host disease progress. Nevertheless, phenomenological prediction of correlation pattern through using taxonomic and genetic repertoire as a proxy of gut content cannot reveal the underlying deterministic processes. Knowledge that demonstrate the non-neutral assembly process of this complex ecosystem is required to develop ecosystem-wide incorporation approach that reveal ecological principles underlying the community assembly, however is still scarce. The challenges impeding theoretical development of decoding gut microbial ecosystem complexity are rooted in three indiscerptible aspects: i) endogenously, the inherently emergent complexity arising not simply due to the inextricable number of components the system is composed of, but rather of time-dependent and non-linear way that these components interact. ii) exdogenously, multi-dimensional host-microbe metabolic axes which contain a series of environmental factors, such as physical force, water absorption, hydrodynamics of colon microenvironment, initial colonization from maternal hand-over and subsequent diet regime that microbiota are exposed to have driven the complex and dynamic development trajectory is crucial while challenging to reveal the hidden ecological forces. iii) Spatial heterogeneity of microbial localization which play a significant impact on microbiota stable colonization and disease development should be incorporated into the model. Therefore, we develop a multi-dimension modeling framework that comprehensively address aforementioned bottlenecks hereby could be used to predict the microbiome shaping landscape and development process from newborn to first year life. Our approach, incorporates both dynamic behavior of individual species and community assembly rules which is supervised by resource allocation strategy in an intelligent way that is updated in context-dependent environment, hereby begetting higher-order community fitness function and elucidating the microbial-microbial interactions from the ecological perspective. The complex interaction network among host, microbiome and diet are implemented through the transport phenomena involving colon longitudinal direction and cross sectional direction that link differentiated phases which would shape the corresponding metabolic dynamics of spatially-specific microbiome. Our approach is applied to predict the infant microbiome evolution process. Both the microbial development process and microbial composition profile at known stages are reproduced by our model, through comparing our prediction of fecal composition with the measured 16S-rRNA data. Besides, our model predict the time-resolved metabolic and microbial profiles inside colon that is not accessible in vivo. Our in silico predictions are consistent with reported phenomena very well. Therefore, the integrative models proposed in our work would be of paramount importance to deconvolvethe embedded complexity and achieve the final controllability by simultaneously encompassing the most challenging issues in gut ecosystem across temporal and organizational scales, contextualized with human physiology.

    Time:
    16:45-17:10

    Title: Evaluation of the Pharmacological Profile of Virgin Coconut Oil (VCO) in Indomethacin-Induced Gastric Ulcer Models

    Parker Elijah Joshua
    University of Nigeria, Nigeria

    Biography
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    Biography

    Parker Elijah Joshua
    University of Nigeria, Nigeria



    Abstract
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    Abstract

    Parker Elijah Joshua
    University of Nigeria, Nigeria

    Several studies have reported various health benefits of virgin coconut oil (VCO) including its use for weight management, treatment of burns, various infections, and even HIV/AIDS. The present study was conducted to evaluate the pharmacological profile of VCO in indomethacin-induced gastric ulcer models. Twenty-four (24) Wistar albino rats were used for the study and were divided into 6 groups of 4 rats each. Group 1 rats served as the normal control; group 2 (positive control) rats were administered with indomethacin only, at a dose of 100 mg/kg b.w. Group 3 rats were treated with a standard drug (cimetidine) at a dose of 100 mg/kg b.w. Groups 4 (3 ml/kg b.w. VCO), 5 (6 ml/kg b.w. VCO) and 6 (9 ml/kg b.w. VCO) rats were treated as stated. The extract (VCO) and the standard drug were dissolved in distilled water, and were administered orally to the rats. Treatment with the standard drug (group 3) and VCO (groups 4, 5 and 6) lasted for four days, followed by indomethacin induction (groups 2 to 6) after an overnight fasting. Four (4) hours after induction, the rats were sacrificed and the blood samples collected for biochemical analyses. Stomach tissues were also harvested for histological examination. The gastric ulcer index of rats in groups 4 and 6 was found to be significantly (p < 0.05) higher compared to that of group 1, while group 5 rats treated with 6 ml/kg b.w. VCO, showed a non-significant (p > 0.05) increase in the gastric ulcer index compared to that of group 1. The ulcer protective indices of low, mid and high doses of the oil were found to be 38.71, 54.84 and 48.39 respectively, which were found to be higher compared to the standard control (6.45). Treatment with VCO resulted in a non-significant (p > 0.05) increase in the gastric juice volume of rats in groups 4, 5 and 6 compared to that of group 1. There was no significant (p > 0.05) decrease in the gastric pH of groups 4 and 5 rats when compared to the normal control. However, a significant (p < 0.05) decrease in the gastric pH was observed when a high dose of the extract was administered. Administration of the extract resulted in no significant (p > 0.05) differences in the lipid profile, lipid peroxidation and antioxidant parameters, compared to the normal control. Histological findings revealed that stomach sections of rats in groups 4 and 5 showed moderate widespread mucosal necrosis and ulceration, while that of group 6 rats showed focal area of mucosal ulceration with evidence of healing by fibrosis when compared to group 1. The findings of this research revealed that virgin coconut oil (VCO) possesses ulcer ameliorative properties and could therefore be used for the treatment of gastric ulcers. The pharmacological properties of virgin coconut oil could be anchored on mechanisms such as the ability to scavenge free radicals, stabilize cell membranes, stimulate mucous and prostaglandin secretion, inhibit Helicobacter pylori and modulate lecithin cholesterol acyltransferase (LCAT) activity.

    Time:
    17:10-17:35

    Title: Determination of Haemolytic Effect and Spectral Analysis Using Gas Chromatography Mass Spectrometry (GC-MS), Fourier Transform Infrared (FTIR) and Ultraviolet Visible (UV-Vis) Spectroscopy of Different Extracts of Cucumis melo L. var. inodorus (Sweet Melon) Fruit

    Parker Elijah Joshua
    University of Nigeria, Nigeria

    Biography
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    Biography

    Parker Elijah Joshua
    University of Nigeria, Nigeria



    Abstract
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    Abstract

    Parker Elijah Joshua
    University of Nigeria, Nigeria

    The concept that fruits and vegetables contribute to a person’s wellbeing is as old as Hippocrates, the father of medicine, who more than 2000 years ago told his patients “let your food be your medicine and your medicine be your food”. Today, this same philosophy with regard to fruits and vegetables being more than just nutrition but medicine as well is experiencing rejuvenation. Indeed, a positive correlation has been reported between fruit consumption and the decreased risk of several chronic diseases including obesity, cardiovascular disease, and certain types of cancer etc. (Boeing et al., 2012; Jansen et al., 2011). The study is aimed at determining the haemolytic effect of Cucumis melo L.var. inodorus (sweet melon or honeydew melon) on human erythrocytes and to study this effect by different spectral analyses.

    Time:
    17:35-18:00

    Title: The Influence of Milky-Wax Ripeness Walnuts Extracts on Formation and Properties of Clots

    Orlova Olga Iurevna
    ITMO University, Russia

    Biography
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    Biography

    Orlova Olga Iurevna
    ITMO University, Russia

    Olga Orlova is a head of the Committee on Innovation and Technology Implementation, a member of international research centre “ Biotechnologies of the Third Millennium”, Associate Professor of the Applied Biotechnology Department at the ITMO University, Russia. She is also Skolkovo Foundation expert, FoodNet adviser in Saint-Petersburg, Leader of the St. Petersburg Project “Nutrition for the Future. She was born on January 31, 1966. Olga Orlova graduated the Institute in 1989. She received her PhD in 2009 from St. Petersburg State University of Refrigeration and Food Engineering. She was the chief technologist at a dairy plant for 12 years. She is married and has 2 children. Scientific interests: food biotechnology, foods for particular nutritional uses, shelf life prolongation.



    Abstract
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    Abstract

    Orlova Olga Iurevna
    ITMO University, Russia

    The aim of this work was to study the influence of chemical composition of extracts from the fruits of milky-wax ripeness walnuts on the formation of lactic acid clots and on the duration of their storage. Experimental studies were carried out in the international research centre "Biotechnology of Third Millennium" Institute of Refrigeration and Biotechnology, ITMO University. Modern methods of research using laboratory equipment of companies Shimadzu, Buchi, Zeiss, Kohler and other were used. Possibility of using of liquid and encapsulated extracts of milky-wax ripeness walnuts in the production of fermented milk drinks was investigated. In this work, we have identified types, the optimal amount of starter culture and the influence of the extracts on growth of microbial flora of drinks. The type, optimal dose and stage of introduction of milky-wax ripeness walnuts extract to raw materials were also defined. Changes in physicochemical properties and chemical composition of extracts were studied. The positive effect of milky-wax ripeness walnuts extract on formation and properties of fermented milk clots was established. Due to naphthaquinone-juglone, which is a part of walnuts extract, fermented milk drinks had a sustainable microbiological condition throughout the period of storage. Thus, using of additives based on milky-wax ripeness walnuts enrich products with substances necessary for the daily prevention of organism from disease and environmental hazards. Developed products can be successfully used to supply of different population groups (children, the elderly, athletes, people working in extreme conditions), as well as in the health-promoting purposes.

  • Sessions:
    Poster Presentations

    Time:

    Title: Native Cell Membrane Nanoparticles System for Membrane Proteins

    Weihua Qiu
    Virginia Commonwealth University, USA.

    Biography
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    Biography

    Weihua Qiu
    Virginia Commonwealth University, USA.

    Weihua Qiu received B.S. degree in biotechnology from Henan Normal University, China in 1999 and Ph.D in biological sciences from the University of Texas at Austin, USA in 2010. She then joined in Rutgers University and Columbia University for postdoctoral training with Drs. Aaron Shatkin and Susan Steinberg. Since 2016, she worked with Dr. YouzhongGuo in Virginia Commonwealth University.Her current research focus on membrane protein structural biology as a lab manager and postdoctoral scientist.



    Abstract
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    Abstract

    Weihua Qiu
    Virginia Commonwealth University, USA.

    We devised a native cell membrane nanoparticles system, which we applied in a single-particle cryo-EM study of the multidrug exporter AcrB. Lipid-AcrB nanoparticles were prepared directly from membranes without any use of detergents. A 3D reconstruction in C1 symmetry achieved a final density map at 3.2 Å resolution, an atomic model of quasi-C3-symmetric AcrB was fitted to this map, and the residual density revealed many ordered lipid molecules. Most remarkably, a central cavity between the three transmembrane domains contains a 24-lipid patch of well-ordered bilayer structure. Inner leaflet lipid chains pack in a hexagonal array like that in phosphatidylethanolamine crystal structures, whereas the outer leaflet has highly irregular packing. Protein side chains interact with both leaflets and participate in the hexagonal pattern. The AcrB export mechanism requires reorganization of the lipid bilayer structure. This system should be broadly applicable for membrane protein structural biology and structure-based drug discovery and development.

    Time:

    Title: Microbial Production of Natural 2-Phenylethanol

    Karolina Chreptowicz
    Warsaw University of Technology, Poland.

    Biography
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    Biography

    Karolina Chreptowicz
    Warsaw University of Technology, Poland.

    Karolina Chreptowicz is currently a Ph.D. candidate at the Warsaw University of Technology. She earned her Bachelor and then Master degree in Industrial Biotechnology at the Warsaw University of Technology, Faculty of Chemistry, Poland. Since 2013, she has been working with dr Jolanta Mierzejewska in the field of yeast biotechnology. At present, in her Ph.D. thesis, she is involved in the development of a laboratory-scale technology for the production of natural 2-phenylethanol - starting from the biotransformation stage by separating and purifying the final product.



    Abstract
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    Abstract

    Karolina Chreptowicz
    Warsaw University of Technology, Poland.

    Thanks to its pleasant rose flavor as well as antibacterial and antifungal properties, 2-phenylethanol (2-PE) has huge market demand. After vanillin, it is the second-most-used additive in perfumery, while also being a component in the food and pharmaceutical industries. Although nowadays most 2-PE originates from chemical synthesis, biotechnological production with yeast is becoming increasingly more attractive since it gives a final product classified as natural. The presented work is focused on microbial production of natural 2-PE. The first stage of the study was the search for yeast strains, isolated from the natural environment, that will efficiently produce 2-PE. With the best producer, we have developed a complete technology of 2-PE production - starting from the biotransformation stage and ending with pure product. In a batch culture conducted in a 5-l bioreactor, we obtained 3.6 g/l 2-PE after 72 h. As 2-PE titer of 2-4 g/l in broth is toxic for yeast, we achieved maximum concentration in a simple batch-culture. Therefore, to enhance productivity we tested extractive fermentation as one of several in situ product removal (ISPR) techniques. We showed for the first time that rapeseed oil can be successfully applied for this purpose. In addition, it is also an excellent biomaterial with promising use in the food or cosmetic industries. Recently, our work has focused on reducing the production costs and, to this end, we tested organic waste from agriculture and food processing as cheap feedstock. This approach has two advantages: lowering the price of the culture medium and better management of harmful wastes. Currently, we are working on downstream processing to combine all stages in one complete technology. This work was financially supported by the National Science Centre, Poland (2016/21/D/NZ9/01605).

    Time:

    Title: Determination and Quantification of Heavy Metals using Infrared Spectroscopy and Chemometric Techniques

    Raul Delgado Macuil
    CIBA, IPN, Mexico

    Biography
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    Biography

    Raul Delgado Macuil
    CIBA, IPN, Mexico

    Raul Delgado Macuil born in Puebla Mexico. He received B.S. degree in electronics from BUAP by Physical-Mathematics School in 1994; and he received the PhD in Optics from Astrophysical Optics y Electronics National Institute in 2005; both institutions in Mexico. Head research in the nanobiotechnology and biosensors fields in the Applied Biotechnology Research Center, a National Politechnique Institute center in Mexico. As author or co-author, about 70 scientific and professional papers have been published. He is a head of 21 national projects and more than 25 graduate and postgraduate thesis has been directed, have more than three thousand nationals and international congress participations.



    Abstract
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    Abstract

    Raul Delgado Macuil
    CIBA, IPN, Mexico

    Actually the determination and quantification of heavy metals in food has a high priority for public health, quality systems and food safety, these elements can be added to the food production system at different stages of the agri-food chain and it is essential to monitor his presence or absence through it. Until now, the methodology to perform this task is based on atomic absorption spectrophotometry that requires at least a couple of days to obtain a reliable result. The aimed of this work is develop and validate an alternative analytical method for the determination of heavy metals in several matrices, based on numerical methods and the correlation between spectrometric techniques; Atomic Force and Infrared by Fourier transform. The presence of Hg and Pb at different concentrations seem to modify the milk spectrum in the region of proteins, lipids and OH; at 1636, 1337, 550, 3300 and 3350 cm-1.The presence of As, Cd, Cr Hg and Pb at concentrations even of 0.1 ppb generate characteristic IR spectra that can help to identify their presence in water. By Principal Components Analysis (PCA), was possible have a better discrimination of the samples in the same matrix (milk).Where the cloud of points in the different regions of interest, show a good discrimination for the seven metals used in this work.

    Time:

    Title: Solid-Phase Enzyme Catalysis 0f DNA End Repair Avoids Heat Treatment and Reduces GC-Bias in Next-Generation Sequencing of Human Genomic DNA

    Aihua Zhang
    New England Biolabs, USA

    Biography
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    Biography

    Aihua Zhang
    New England Biolabs, USA

    Dr. Aihua Zhang graduated from Hunan medical college, Hunan, China in 1984 and did her postdoctoral training at MGH, Harvard U.S.A. she has been working as a research scientist at New England Biolabs, Inc. since 1995. Her current research interest is validating immobilized enzymes for NGS library prep.



    Abstract
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    Abstract

    Aihua Zhang
    New England Biolabs, USA

    Next-generation sequencing (NGS) has caused a revolution in both research and diagnosis. NGS analysis relies on preparation of a representative, non-biased library evenly distributed across the entire genome under investigation. This critical task has become increasingly challenging since biases found in the current methods of the NGS library preparation produce uneven coverage and compromisethe quality of NGS analysis. In this report we have identified a systematic sequence bias during construction of amplification-free human DNA librariesfor the Illumina sequencing platform. Our study indicatesthat inefficient processing of AT-rich DNA in the major steps that comprise library construction resultsin under-representation of the extremely high AT-content fraction in human genomic libraries. We have demonstrated a new strategy by employingDNA modifying enzymes conjugated to magnetic beads in construction of amplification-free human DNAlibraries. We show that this method significantly lowers the sequence coverage bias of the libraries on Illumina sequencing platform.

    Time:

    Title: The Mechanism of Action of Zingerone in the Pacemaker Potentials of Interstitial Cells of Cajal in Murine Small Intestine

    Byung Joo Kim
    Pusan National University School of Korean Medicine, Republic of Korea

    Biography
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    Biography

    Byung Joo Kim
    Pusan National University School of Korean Medicine, Republic of Korea

    Byung Joo Kim, has completed his PhD from Seoul National University and also postdoctoral studies from Seoul National University. He is the Professor at the Division of Longevity and Biofunctional Medicine, Pusan National University School of Korean Medicine, Yangsan, Republic of Korea. His research interest is in the action mechanisms of traditional medicine and interstitial cells of Cajal in gastrointestinal tract.



    Abstract
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    Abstract

    Byung Joo Kim
    Pusan National University School of Korean Medicine, Republic of Korea

    Background: Zingerone, a major component found in ginger root, is clinically effective for the treatment of various diseases. Interstitial cells of Cajal (ICCs) are the pacemaker cells responsible for slow waves in the gastrointestinal (GI) tract.We investigated the effects of zingerone on the pacemaker potentials of ICCs to assess its mechanisms of action and its potential as a treatment for GI tract motility disorder. Methods: We isolated ICCs from small intestines, and the whole-cell patch-clamp configuration was used to record the pacemaker potentials in cultured ICCs. Results: Under the current clamping mode, zingerone inhibited pacemaker potentials of ICCs concentration-dependently. These effects were blocked not by capsazepine, a transient receptor potential vanilloid 1 (TRPV1) channel blocker, but by glibenclamide, a specific ATP-sensitive K+channel blocker. Pretreatment with SQ-22536 (an adenylate cyclase inhibitor), LY294002 (a phosphoinositide 3-kinase inhibitor), and calphostin C (a protein kinase C (PKC) inhibitor) did not block the effects of zingerone on the pacemaker potentials relative to treatment with zingerone alone. However, zingerone-induced pacemaker potential inhibition was blocked by 1H [1,2,4] oxadiazolo [4,3 a] quinoxalin 1 one (ODQ; a guanylate cyclase inhibitor), KT5823 (a protein kinase G (PKG) inhibitor), and L-NAME (a non-selective nitric oxide synthase (NOS) inhibitor). In addition, zingerone stimulated cyclic guanosine monophosphate (cGMP) production in ICCs. Finally, pretreatment with PD98059 (a p42/44 mitogen-activated protein kinase (MAPK) inhibitor), SB203580 (a p38 MAPK inhibitor), and SP600125 (c Jun N terminal kinases (JNK) specific inhibitor)blocked the zingerone-induced pacemaker potential inhibition. Conclusion: These results suggest that zingerone concentration-dependently inhibits pacemaker potentials of ICCs via NO/cGMP-dependent ATP-sensitive K+channels through MAPK-dependent pathways. Taken together, this study shows that zingerone may have the potential for development as a GI regulation agent.

    Time:

    Title: Determination of Optimal Sterilization Types for In Vitro Propagation of Walnuts Cultivars in Georgia

    Iveta Megrelishvili
    Georgian Technical University, Georgia

    Biography
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    Biography

    Iveta Megrelishvili
    Georgian Technical University, Georgia

    IvetaMegrelishvili has completed his PhD at the age of 28 years from IvaneJavakhishvili Tbilisi State University. She is the main research scientist of Georgian Technical Univeristy, Biotechnology Center and Head of Virology Lab, Scientific-Research Center of Agriculture. He has published more than 8 papers in reputed journals and has a great experiences in the field of plant biotechnology, plant virology and molecular biology.



    Abstract
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    Abstract

    Iveta Megrelishvili
    Georgian Technical University, Georgia

    Microbial diseases have been reported in the walnut orchards due to different reasons in recent years in Georgia. For the purpose of walnuts tissue cultures reproduction, it is necessary to determine the optimal type of sterilization of initial material. The aims of this study were determined optimal sterilization type to propagate walnuts cultivars ‘Pedro’, and ‘Chandler’ using tissue culture technique. The initial explants (unimodal micro cuttings with a length between 1 and 1.2 cm) of three cultivars were collected from the young 2-3 years old walnuts orchards, Dzevera, ShidaKartli, Georgia. Two sterilized types were used for in vitro propagation of walnuts: I.1-2% hypochlorite 10-15 min, followed 70 % alcohol -30 min and 3 times sterile distillate water II. 0.1% mercuric chloride 5 min and 3 times sterile distillate water. It was revealed that microbial contamination were 58.97% using 0.1% mercury chloride and relatively high by sodium hipocloride-85.12%. DKW medium was used for in vitro cultivation of walnuts cultivars supplemented with 0.1 mg/L IBA, 1 mg/L BAP and sucrose 3%. The pH of culture medium was adjusted at 5.5 before adding the gelling agent and autoclaving. Micro cuttings were kept at 25±2oC under a 16 h photoperiod. Finally, optimal sterilization types of initial explants (Combination II) was determined. Walnuts cultivars: ‘Pedro’ and ‘Chandler’ were propagated using tissue culture methods first time in Georgia. This study led to develop an effective method for micro propagation of Juglansregia, which enable us to establish new walnuts orchards in Georgia.

    Time:

    Title: Definition of Optimal In Vitro Conditions for Different Maturity Potato Cultivars

    Maia Kukhaleishvili
    Georgian Technical University, Georgia

    Biography
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    Biography

    Maia Kukhaleishvili
    Georgian Technical University, Georgia

    Maia Kukhaleishvili has completed his PhD at the age of 58 years from ST. Andrew the First Called Georgian University of the Patriarchate of Georgia. She is the director of Georgian Technical University, Biotechnology Center- Scientific-Research Center. She has published more than 10 papers in reputed journals and has a great experiences in the field of Agriculture and Biotechnology.



    Abstract
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    Abstract

    Maia Kukhaleishvili
    Georgian Technical University, Georgia

    The research aim was to determine optimal in vitro condition for propagation different maturity potato cultivars. Potato varieties were collected from in vitro potato collection of Georgian Technical University, Biotechnology Center according to their maturity: Early cultivars: Viviana, Red Sonia, Bellarosa, Vineta, Anushka; Medium early:Donata, Bernina, Madeira, Sante, Laura. Medium late: Brodie, Shepody,Jelly, Carola, Desiree. The influence of three types of combinations with temperature, humidity, light and photoperiod was studied on all three maturity potato cultivar’sin vitro development:I. T-23-250 C, H-80%, Lux-5-5500, 16h; II. T-25-270 C, H-75%, Lux-5-5500,16h: III. T-27-290 C, H-70%, Lux-5-5500, 16h;The results were evaluated after 17 days of reproduction. Best in vitro condition for all researched potato varieties was selected for their leave colors, rooting, and shoot formation. All potato cultivars morphological characterization was variable depending on the type of in vitro condition. It was revealed early maturity cultivars had maximum potential for in vitro propagation (5-6 nodes, average rooting 92% and shoot formation 94%) on combination: T-23-250 C, H-80%, Lux-5-5500, 16h. Combination: T-25-270 C, H-75%, Lux-5-5500,16h was best for medium early cultivars (5-6 nods, rooting 89% and shoot formation 90%). Theperfect in vitro reproduction of medium late varieties( 5-6 nodes, average rooting 87% and shoot formation 82%) was observed on the combination: T-27-290 C, H-70%, Lux-5-5500, 16h. As it is known, for in vitro developments of early medium and late medium potato cultivars are necessary 22-26 days, but our results were obtained in 17 days. Finally, optimal in vitro condition for propagation different maturity potato cultivars was defined.

    Time:

    Title: Affinity Protein Purification Resulting in Protein Sequence without Remaining Amino Acid Residues

    Heba Alaa Eldeen Hosiny Abd Elhameed
    University of Szeged, Hungary

    Biography
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    Biography

    Heba Alaa Eldeen Hosiny Abd Elhameed
    University of Szeged, Hungary

    HebaAlaaEldeenHosinyAbdElhameedis a PhD student in the University of Szeged.She is from Egypt, Where she has been working as Assistant lecturer atZagazig University.Presently she is examining new types of artificial nucleases by means of various biophysical methods within the frame ofa StipendiumHungaricumPhD scholarship and under supervision of Dr. Bela Gyurcsik.



    Abstract
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    Abstract

    Heba Alaa Eldeen Hosiny Abd Elhameed
    University of Szeged, Hungary

    Purification of proteins is vital for the characterization of the function, structure and interactions of the protein of interest and efficiently carried out by affinity chromatographic methods. The specific interaction with the appropriate resins requires fusion affinity tags, such as e.g. the oligo-His, the maltose binding protein or glutathione-S-transferase tag. These sequences are encoded by the plasmids used for protein expression. The affinity tags have to be cleaved off after the target protein is selectively bound to the solid support. This is performed by specific proteases. These enzmes are expensive and mostly they leave few extra amino acids at the terminus, which may interfere with the structure and function of the purified protein. Recent studies in our research group focused on the protein constituents of new artificial nucleases. Artificial DNA nucleases have provided scientists with the unprecedented ability to probe, regulate, and manipulate the human genome. Zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and the clustered regularly interspaced short palindromic repeat-Cas9 system (CRISPR/Cas9) represent a powerful array of tools that can bind to and cleave a specified DNA sequence. Our ressearch focused on new type of ZFNs with intramolecular alloteric activation. The computer design of such enzymes requires the precise protein sequence to be obtained. Using the Ni(II)-affinity chromatography for e.g. zinc finger protein purification requires the complete removal of the oligo-His affinity tag. However, this was not possible to achieve by the traditional methods. We redesigned the cloning region of pET21a plasmid. The new approach is to cleave the His-tag by Ni(II) instead of proteases. Therefore, the new plasmid encodes the affinity tag with a Ni(II)-sensitive cleavage site at its N terminus. The precise gene of the protein is inserted into the cloning region by the help of BsmBI restriction endonuclease, so that the recognition site is deleted in the cleavage/ligation procedure. We performed the cloning, expression and purification procedures and will report the results on the poster.

    Time:

    Title: Development of the Method of Isolation, Culturing and Assessment of Metabolic Ability of Bacteria from Species Desulfovibrio Desulfuricans

    Katarzyna Ciemniak
    Poznan University of Life Sciences, Poland

    Biography
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    Biography

    Katarzyna Ciemniak
    Poznan University of Life Sciences, Poland

    Katarzyna Ciemniak iscurrently a Ph.D. candidateat the Poznan University of Life Sciences. ShecompletedherEngineer’s and thenMaster’sdegree in IndustrialBiotechnologyat the Department of Biotechnology and Food MicrobiologyatPoznan University of Life Sciences, Poland. Since 2017 shehasbeenworkingwith dr hab. Daria Szymanowska – Powałowska in the field including pharmaceutical chemistry and microbiology, carrying out tests forbacterialresistance on complexes of antibiotics with cyclodextrins. Since the beginning of herdoctoralstudiesshehasbeenparticipating in aprojectconcerningresearch on the activity of Desulfovibriodesulfuricansbacteria in the process of decomposition of petroleum-derived waste.



    Abstract
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    Abstract

    Katarzyna Ciemniak
    Poznan University of Life Sciences, Poland

    Bacteria of the genusDesulfovibrioare gram-negativeabsoluteanaerobicmicroorganisms, usuallyrod-shaped. Theyhave the ability to carry out the processes of dissimilatoryreduction of sulphates to hydrogensulphidetaking part in the sulfurcycle in nature. A typicalspecies of thisgenusisDesulfovibriodesulfuricans, occurringmainly in deeperlayers of soil, sediments of water, industrialsewage, oilfieldsas well asanimal and humanstool, sometimescausinginfections of the digestive system. The aim of the work was to develop a procedure for the isolation of Desulfovibriodesulfuricans from environmentalsamplesusingvarious selection factors. In addition, the metabolism of isolates have been tested, including their ability to grow in substrates with various carbon sources and the utilization of diesel fuel as the only carbon source as well as the ability to reducesulphates. The tests were carried out under relatively anaerobic conditions using soil samples contaminated with petroleum derivatives. Tests showing metabolic abilities were carried out in bioreactors maintaining sterility regimes and appropriate gas conditions. The increase in biomassconcentration,substrateloss and sulphatereductionswereanalyzed. As a result of the work, Desulfovibriodesulfuricansmonocultureswereobtainedusing the antibiotic selection factor. The conducted culturing tests allowed for the selection of parameters allowing for an efficient biomass multiplication as well as the assessment of the influence of parameters such as pH, temperature, agitator speed and gas conditions on the metabolicconditions of Desulfovibriodesulfuricans. In addition, the ability of bacteria to utilize diesel oil and to reducesulphate was analyzed.

    Time:

    Title: Violacein as a Bioactive Compound of Microbiological Origin - Bioproduction and Anti- Melanoma Activity

    Patrycja Kowalska
    Warsaw University of Technology, Poland

    Biography
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    Biography

    Patrycja Kowalska
    Warsaw University of Technology, Poland

    Patrycja Kowalska is currently a MSc.Eng. student at the Warsaw University of Technology. She is going to specialized in Medicine and Cosmetics at Faculty of Chemistry WUT. She earned her Bachelor’s Engineering degree in Biotechnology at the Warsaw University of Technology, Faculty of Chemistry, Poland. During her BSc studies she started working with Ph.D. Małgorzata Milner-Krawczyk in the field of bacteria biotechnology and violacein bioproduction. Nowadays studies ordinance on cell lines studies and potentially used of violacein.



    Abstract
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    Abstract

    Patrycja Kowalska
    Warsaw University of Technology, Poland

    Violacein is an organic chemical compound that is derived from indole. Microorganisms capable of producing violacein are characterized by a dark purple coloration of the colonies. As shown in recent years, this compound has antibacterial, antifungal and anticancer properties. Studies on violacein's influence on various cell lines, conducted by many independent teams, indicate that it acts on tumor lines in small concentrations, and the mechanism of its action is depended on the cell type. In some cases, violacein induces apoptosis in cancer cells without affecting normal cells. We isolated new strain, capable to violacein bioproduction. On the basis of comparative analysis of the 16S rRNA coding sequence the strain was classified as Janthinobacterium lividum (KP16). We established optimal culture conditions, as well as extraction and purification methods of this intracellular secondary metabolite: 2-fold diluted lysogenic broth (LB) as a growth medium and 5 days culture at 15 – 20 ˚C with shaking at 110-120 rpm. The obtained methanolic extracts were purified in the system of acetone : chloroform : ammonia (1 : 2 : 0.01) on a silica beads (Silica gel 60 with grains 0.015 - 0.040 mm). After optimizing the extraction and purification methods, the violacein was obtained with a purity of over 95%. Subsequently, studies concerning proliferative and metabolic activity of violacin-treated melanoma cell lines were performed. The results showed a clear selective effect of violacein on metastatic cells.

    Time:

    Title: Separation of Proteins on Ion-exchange Resins

    Aleksandra Zawodnia
    Poznan University of Life Sciences, Poland

    Biography
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    Biography

    Aleksandra Zawodnia
    Poznan University of Life Sciences, Poland

    Aleksandra Zawodnia is a graduate student of the Poznan University of Life Sciences (Poland). In 2017 she earned M.Sc. degree in Biotechnology, on the Faculty of Agriculture and Bioengineering. At present, she works as a microbiologist at Poznan University of Life Sciences, Department of Biotechnology and Food Microbiology. She is interested in industrial biotechnology and environmental microbiology.



    Abstract
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    Abstract

    Aleksandra Zawodnia
    Poznan University of Life Sciences, Poland

    Lactoferrin (Lf) is a biologically active glycoprotein from the transferrin family, mainly found in milk. Lf exhibits antimicrobial, immunomodulating and anti-cancer activity. Lab-scale experiments were carried out to generate input data for simulations of an industrial plant for Lf production. Lf was purified using an ion exchange chromatography.Three ion exchange resins have been tested: Amberlite XAD761, Dowex 50WX4 and SP-Sepharose. Two different elution methods were employed: linear gradient and step gradient. The bicinchoninic acid protein assay method with BSA protein standard was used for further analysis of protein fractions.The composition of the different fractions was investigated by using SDS-PAGE electrophoresisto assess their purity. The conceptual project and economic analysis of a plant for production of LF were done using the software SuperPro Designer version 10. Best results were obtained when SP-Sepharose was usedas an stationary phase leading to a total recovery of Lf from column. The resin capacity determined using pure Lf was equal to 60 mg Lf/ml.The linear gradient resulted in the separation of lactoferrin into Lf a and Lf b, while in the step gradient, only one peak of lactoferrin was obtained. However, the most favorable was separation in step gradient, becausein this method, higher concentrations of lactoferrin were obtained. Simulation results performed in SuperPro Designer indicate that the step method was more economical than the linear gradient method; therefore changing the Lf production method to step could reduce the cost of Lf production from milk whey.

    Time:

    Title: Development of Eubiotic Preparation for Domestic Livestock

    Aleksandra Wanczyk
    Poznan University of Life Sciences, Poland

    Biography
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    Biography

    Aleksandra Wanczyk
    Poznan University of Life Sciences, Poland

    Aleksandra Wanczyk is a graduate with a Master’s Degree in Biotechnology at Poznan University of Life Sciences in Poland. She is currently working as a microbiologist at the Department of Biotechnology and Food Microbiology of Poznan University of Life Sciences. Her research interests are in the field of yeast biotechnology.



    Abstract
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    Abstract

    Aleksandra Wanczyk
    Poznan University of Life Sciences, Poland

    Animal feeding is a key element in cost-effective production. beneficial effects can be achieved with wholesome feeds based on high quality roughage and nutritious fodder, vitamin and mineral additives. Yeast are industrially interesting microorganisms. Their dry weight is composed of lysine- and threonine–rich protein in 35-65%. Yeast are a rich source of B-complex vitamins and ergosterol. They contain fat and carbohydrates which give them high energy content. They are also easily digested. Herbs are also functionally interesting. Their preparations contain beneficial substances, such as flavonoids, anthocyanines, glycosides, tannin and tannines, secoiridoids, mucilage, essential oils, alkaloids, polyphenols and mineral salts. These compounds have antistress, antibacterial, antiviral and antifungal properties, stimulate the appetite of livestock and help maintain physiological balance. Oils of thyme, basil and cumin enhance appetite, stimulate secretion of bile and digestive enzymes, and intestinal motility, which improves assimilation of nutrients. Thyme, basil, cumin and camomile oils show activity against pathogenic bacteria, including Clostridium perfringens and E.coli. The aim of this work was to develop a multifunctional eubiotic preparation composed of two species of yeast and a herbal complex. Fed-batch cultures were conducted in 6.6 L bioreactors. Glucose, molasses, sucrose, whey and corn steep liquor were investigated as substrates. Growth of biomass, substrate utilization and concentration of metabolites were monitored. Kinetic indicators were calculated in order to allow comparison of substrates. From various tested herbal additives, garlic, oregano and thyme extracts were selected based on the results of antioxidant potential and antibacterial activity. Addition of these extracts can improve digestion, nutrient assimilation and immunity of farm animals. This work is the result of cooperation with BAF Inc. for the purpose of the project entitled “Development of a novel recipe and production process of an eubiotic preparation containing two symbiotic yeast species”. Research was financed by POIR.02.03.02-30-0047/17.

    Time:

    Title: Development of Multifunctional Diet Supplements Containing Alpha-Ketoglutaric Acid

    Paulina Maciejewska
    Poznan University of Life Sciences, Poland

    Biography
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    Biography

    Paulina Maciejewska
    Poznan University of Life Sciences, Poland

    Paulina Maciejewska graduated from Poznan University of Life Sciences in Poland, where she received M.S. degree in Biotechnology on the Faculty of Agriculture and Bioengineering in 2017. She works at University of Life Sciences in Poland, Faculty of Food Science and Nutrition, Department of Biotechnology and Food Microbiology as a microbiologist. She is involved in the development of microbiological synthesis of alpha-ketoglutaric acid. She is interested in industrial biotechnology and food microbiology.



    Abstract
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    Abstract

    Paulina Maciejewska
    Poznan University of Life Sciences, Poland

    α-Ketoglutaric acid (KGA) is an organic compound containing both a carboxyl and a ketone group. KGA, also referred to as 2-oxoglutamate, 2-ketoglutaric acid, 2-oxoglutaric acid, is a rate-determining intermediate in the tricarboxylic acid cycle and amino acid metabolism and has a crucial role in cellular energy metabolism. The aim of this project was to develop a new line of probiotics aimed at 5 groups of recipients - athletes, people after antibiotic therapy and chemotherapy, future mothers and children with gut and psychology syndrome (GAPS). The developed products are multi-component liquids and gels, which makes them a novelty on the market, as most probiotic supplements are dry. Bacteria and bacterial metabolites (including KGA), vitamins and minerals and plant extracts with proven health-promoting properties are the functional ingredients of the products. Combination of probiotic bacteria and KGA is another reason that makes the developed product assortment unique on domestic market of functional food. Four research tasks related to the development of the microbiological process of KGA synthesis and the production technology of new probiotic products containing KGA were undertaken in order to achieve the assumed goal. This work is a result of cooperation with Living Food sp.z.o.o., which implements the project entitled "The development of new functional products produced on the basis of a consortium of probiotic strains, alpha-ketoglutaric acid and a vitamin-mineral complex." The work is financed by the National Center for Research and Development. No. Application for co-financing: POIR.01.01.01-00-0685/17.

    Time:

    Title: Direct Screening and Isolation of Microorganisms for a Biofertilizer Formulation

    Laura Jeannette Garcia Barrera
    CIBA-IPN, Mexico

    Biography
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    Biography

    Laura Jeannette Garcia Barrera
    CIBA-IPN, Mexico

    M. C. Laura Jeannette García Barrera is a Researcher of CIBA-IPN, Mexico. She studied Environmental Engineering at UPAEP, Mexico. She has a Master degree in Sciences in the specialty of Biotechnology from CINVESTAV, Mexico. Has experience evaluating the antifungal activity of plant extracts and works with molecular biology of viruses, bacteria and fungi. Has participated in projects in collaboration with the industry and is currently studying the use of biofertilizers and their impact on microbial communities in soils.



    Abstract
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    Abstract

    Laura Jeannette Garcia Barrera
    CIBA-IPN, Mexico

    Among genomic tools, metagenomics hasthepotential for characterizing large-scale population of bacterial genomes from environmental samples, without the need of isolation and growth. In this work, a metagenomic survey for bacteria present in three different substrates, namely soil, compost and digestate, were carried out. Bacteria belonging to genus with agricultural importance, such as plant growth promotion (PGP), were selected for further analysis. Briefly, bacteria with putative PGP activities were isolated and cultivated toward the goal of formulating a biofertilizer. Samples from soil, compost and digestate were culture in selective media (NBRIP, Pikovskaya Agar, Ashby Agar, NFB, R2A, FMA and Chitin Agar for the isolation of Lysobacter, Varioborax, Azospirillum, and Paenibacillus).A total of 152 strains were isolated, corresponding to 47, 42 and 63from soil, compost and digestate; respectively.Then, pure bacterial strainswere assessed as PGP agents by evaluating the production of indol aceticacid, gibberellic acid, and siderophore, as well as seed germination. Forty bacterial strains that showed PGP activity were tested for antagonistic behaviors. At last, 17strains were selected for PGP effects on Dactylisglomerata L.Strains with the higher PGP activities were identified as Microbacterium sp., Enterobacter ludwigii and Rahnella aquatilis. Taken together, the combination of metagenomic information and classical lab procedures allowed to formulate a novel biofertilizer for plant growth promotion, which is the first step to improve crop productivity.

    Time:

    Title: Digestate Concentration and Harvest Intervals Effects On Yield Of Forage Grasses And Legumes

    Laura Jeannette García Barrera
    CIBA-IPN, Mexico

    Biography
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    Biography

    Laura Jeannette García Barrera
    CIBA-IPN, Mexico

    M. C. Laura Jeannette García Barrera is a Researcher of CIBA-IPN, México. She studied Environmental Engineering at UPAEP, México. She has a Master's degree in Sciences in the specialty of Biotechnology from CINVESTAV, México. Has experience evaluating the antifungal activity of plant extracts and works with molecular biology of viruses, bacteria and fungi. Has participated in projects in collaboration with the industry and is currently studying the use of biofertilizers and their impact on microbial communities in soils.



    Abstract
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    Abstract

    Laura Jeannette García Barrera
    CIBA-IPN, Mexico

    Knowing the effect of digestate that it’s the anaerobic digestion subproduct of solid residues of cattle, on the yield of forage grasses and legumes, is a justification to reduce the use of agrochemicals. The aim was to evaluate the effect of concentration, harvest frequency and irrigation frequency on the components of the yield of Ryegrass and White clover. To do this, evaluate digestate concentrations (20, 40 and 60%), harvest frequencies (4, 5 and 6 weeks) and irrigation frequencies (15 and 30 days) were applied. The variables were: Dry matter, weight of leaves, stems, petiole, plant height, growth rate of the crop. The digestate was acquired in Chapingo University. A factorial design with arrangement 3*3*2 was used, and mean comparisons were made by Tukey (P<0.05), by the GLM procedure of the SAS software. The results showed that the highest yield, weight of leaves, stems, growth rate of the crop and height of plant was with the treatment of 60% of digestate, harvest frequency every four week and the irrigation every 15 days in both species (P<0.01). It is concluded that the concentration, harvest frequencies and digestate irrigation frequencies affect the yield and can be used as substitute of agrochemicals fertilizer.

    Time:

    Title: Natural Herbs for the Treatment of Skin Ulcers in Diabetic Animal Models

    Diana Sami
    The American University in Cairo, Egypt

    Biography
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    Biography

    Diana Sami
    The American University in Cairo, Egypt

    Diana Sami is currently a graduate student in the Biotechnology program at the American University in Cairo (AUC). She earned her Bachelor degree in pharmaceutical Science from faculty of pharmacy,Helwan University, Egypt in 2011. She then obtained aDiploma in healthcare and hospital management from the American University in Cairo (AUC) in 2014. At present, Diana is interested in the development of cost effective treatment for skin ulcers using natural herbs.



    Abstract
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    Abstract

    Diana Sami
    The American University in Cairo, Egypt

    Chronic skin ulcers are commonly associated with elderly, bedridden, and debilitated patients resulting from pressure or Diabetes Mellitus. They affect nearly 2 million people each year and account for an annual healthcare cost of about $50 billion. In this study we investigated the wound healing activity of natural herbs (e.g., Turmeric Ginseng, Oregano) in diabetic rats. Turmeric, Ginseng and Oregano extracts were tested in vitro for their antibacterial activity. They were also applied as ointment once daily to skin ulcers in diabetic rats. All herbal extracts showed antibacterial activities against both Gram positive and Gram negative bacteria, with Oregano being the most potent, with nearly 100% bactericidal effect at 0.1% concentration. In vivo results showed that 6% Turmeric enhanced wound-healing with nearly 50% decrease in wound area at day 5 as compared to control group. Oregano also, improved tissue granulation and reduced wound infection at 0.1% as compared to control group. In conclusion, herbal antimicrobials are promising wound-healing agents as they exhibit antibacterial, anti-inflammatory effects, and promote skin healing.

    Time:

    Title: Novel Antibacterial and Anti-Fungal Agent from Eicchornia Crassipes

    Bharat kwartra
    St. Mark’s Sec Public school, India

    Biography
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    Biography

    Bharat kwartra
    St. Mark’s Sec Public school, India

    Bharat Kwatra is a Student of High school in India, but very keen and enthusiastic about microbes and research related to biochemistry, which make him different from others. He is an active Member of American Microbial society, Indian microbial society, European Research trade and development society and World Association for Scientific Research and Technical Innovation at very young age. He is very passionate about his research which encourages him to look forward for gaining knowledge. From the age of 15 years, he is involved in doing research on various health issues. He received first prize in international conference, 2017 and Won Youth research forum at beneficial microbes, USA. He is also being awarded as Youngest Researcher in his state. He has published Three international journals and have seven patents. He is being recently working on Single cell protein with Department of microbiology at his school.



    Abstract
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    Abstract

    Bharat kwartra
    St. Mark’s Sec Public school, India

    Bacterial and fungal infection in humans and plants is an undiscriminating concern to countries worldwide.Large number of it occurs in developing countries with poor economic condition.Eichhornia crassipes, also known as water hyacinth in English, is a floating hydrophytic plant that is notorious for its invasive ability. Due to its prolificness, the plant constantly and quickly causes eutrophication and retardation of the flow of the water it lives in. This results in the need of constant removal of this plant by the people.This research has led to the discovery of using the abundantly found Eichhornia crassipes, as a cheap yet effective source of antibacterial and Antifungal agent.Method and Experiment Design The experiment was conducted in two sets, each set is divided into two parts and each part was subdivided into three elements of different microbes. SET 1, Part 1 1. The ethanol extract of Eichhornia crassipes is first purified through centrifugation and pasteurisation, and then diluted into 3 different concentrations; 75%, 50% and 25%. In three trials, each of the three Eichhornia crassipes extract concentrations is tested against the three bacterias : Staphylococcus aureus, Streptococcus sobrinus and Escherichia coli, in vitro, by the cylinder plate assay method. 2. Water is used as negative control and ampicillin is used as positive control and comparison, being a representative of conventional antibacterial agents. 3. The size of zone of inhibition produced after incubation will be the measure of the antibacterial effect of the extract. SET 1, Part 2 1. The ethanol extract of Eichhornia crassipes is first purified through centrifugation and pasteurisation, and then diluted into 3 different concentrations; 75%, 50% and 25%. In three trials, each of the three Eichhornia crassipes extract concentrations is tested against the two fungi: Magnaporthe grisea, Fusarium oxysporum and Aspergillus niger, in vitro, by the cylinder plate assay method. 2. Water is used as negative control and fluconazole is used as positive control and comparison, being a representative of conventional Anti fungal agents. 3. The size of zone of inhibition produced after incubation will be the measure of the Anti fungal effect of the extract. SET 2, Part 1 A second set of experiment is conducted to test whether the extract is bacteriostatic or bactericidal. The second experiment involves: 1. The transfer of the inhibition zone liquid into a new nutrient media. 2. Presence of bacteria on the new media after 24 hours incubation will determine the type of the antibacterial activity. SET 2, Part 2 A second set of experiment is conducted to test whether the extract is fungistatic or fungicidal. The second experiment involves: 1. The transfer of the inhibition zone liquid into a new nutrient media. 2. Presence of fungus on the new media after 24 hours incubation will determine the type of the antibacterial activity. Result and Analysis SET 1, Part 1 The negative control shows no inhibition effect, indicated by the absence of inhibition zone. The highest inhibition effect is produced by the 75% concentration,. The smallest inhibition effect is produced by the 25% concentration. The Ampicillin produces higher inhibition effect than the extracts. SET 1, Part 2 The negative control shows no inhibition effect, indicated by the absence of inhibition zone. The highest inhibition effect is produced by the 75% concentration,. The smallest inhibition effect is produced by the 25% concentration. SET 2, Part 1 Presence of bacteria in the new nutrient media of the experiment indicates that the extract is bacteriostatic. SET 2, Part 2 Absence of fungi in the new nutrient media of the experiment indicates that the extract is Fungicidal. Three new molecules were derived from the extracts using Beta lactamase test, lanosterol tests and various other tests. Conclusion: The antibacterial property of Eichhornia crassipes is due to the presence of two bioactive chemicals(derived) ; flavonoid and alkaloid. The Anti fungal property of Eichhornia crassipes is due to the presence of bioactive chemicals (derived) similar to fluconazole. Eichhornia crassipes extract possesses antibacterial and Anti fungal properties, and is bacteriostatic and fungicidal respectively. Therefore, Eichhornia crassipes is a good and cheaper substitute of antibacterial due to its easy accessibility and abundance.

    Time:

    Title: A Novel Alpha(1-2)-Fucosylation and Actin Dependent Uptake Vehiculates Doxorubicine-Loaded Liposomes into Highly Proliferative Cells

    Domenico Nolfi
    University of Siena, Italy

    Biography
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    Biography

    Domenico Nolfi
    University of Siena, Italy

    Domenico Nolfi was born in Sicily on the 12th of December, 1991. He is currently undertaking a PhD at the department of Life Sciences of the University of Siena, where he earned a master's degree in Molecular and Cellular Biology in 2016 after completing a bachelor's degree in Biological Sciences in 2014. His fields of interest are Glycobiology and Cellular Biology and his research focuses on a alpha-2 fucosylation-dependent uptake machanism and its role in targeting of drug delivery systems. In his spare time, he loves cooking and dancing



    Abstract
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    Abstract

    Domenico Nolfi
    University of Siena, Italy

    Finding new strategies to direct drug carrying nanovesicles towards specific cellular targets is one of the major goals of Biomedical Research today. Recently, we found a fucosylated structure exclusive of high proliferative cells, which is responsible for a peculiar uptake mechanism alternative to the classic ones and dependent on alpha(1-2)fucosylation. This structure, detectable by using the fucose-binding lectin from Lotus tetragonolobus (LTL), was first observed in CVEC and then in A431 and DU145. It appears as a network of tubules extending between the perinuclear region and the cellular periphery. LTL binding sites were also found to be exposed at the cell surface in a very restricted region. The uptake capacity of the LTL-positive tubular structure was then demonstrated by testing the lectin with living cells. Other lectins tested in the same way were found to enter the cells through the classical endocytotic mechanism. In order to exploit this new uptake mechanism as drug delivery system, we constructed and tested in DU145 doxorubicin-loaded liposomes functionalized with LTL (LTL-Dox-L). We confirmed that the LTL-Dox-L enters the cells as the corresponding lectin and that by this vector the intracellular delivery of the drug was much more than that entered via unmodified doxorubicin-loaded liposomes. By electron microscopy we also demonstrated that liposomes enter the cells one by one in tiny tubules that never fuse with lysosomes. Therefore, liposome functionalization with LTL promises to broaden the therapeutic potential of liposomal doxorubicin treatment, decreasing non-specific toxicity. More recent results have indicated the actin cytoskeleton as responsible of the integrity of the tubular structure: destruction of actin filaments with Cytochalasin D gives rise to the gradual disappearance of the tubular LTL positive structure, until it remains as vesicles in the perinuclear region. The treatment with Cytochalasin D also strongly decreases the entrance in the cells of LTL-Dox-L.

    Time:

    Title: Evaluation of Phenomenological Variables by Applying Compost and Digestate to Different Concentrations in Lettuce Cultivation (Lectuca Sativa L.)

    Jeisel Delgado Flores
    CIBA-IPN, Mexico

    Biography
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    Biography

    Jeisel Delgado Flores
    CIBA-IPN, Mexico

    Jeisel Delgado Flores is a master's student in the area of Applied Biotechnology at CIBA-IPN, Mexico. She is an engineer in food industries graduated from the InstitutoTecnológico del Altiplano (ITAT), Tlaxcala, México. She has experience in training people from rural communities, to process fruits and vegetables, dairy products and meat as an alternative to food preservation. She also has work as a researcher in conjunction with ITAT to find option to water treatment contaminated with heavy metals. Currently, his research work focuses on the development of a biofertilizer applicable to crops for human consumption.



    Abstract
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    Abstract

    Jeisel Delgado Flores
    CIBA-IPN, Mexico

    Increase in world population has caused an increasing demand for food, especially those of vegetable origin. Consequently, to meet this demand, farmers have used agrochemicals to improve crop yields. The objective of this work was to evaluate the growth of lettuce (Lactuca sativa L.) in response to the application of two types of organic fertilizers: compost and digestate, each of them obtained from organicwaste and cowdung; respectively. Plants with untreated soil were grown as a negative control, while plants grown in the presence of a chemicalfertilizer (NPK 17-17-17) were established as positive controls. In the case of plants treated with compost, concentrations of 20%, 40%, 50%, 60% and 100% were used; while for digestate they were 20%, 40%, 60% and 80% with frequencies of 15 and 30 days. The height of the plant, fresh weight, leaf area and root were measured. The experiments showed that the best compost treatment was the 100% application, in which the height of the plants was 52% higher and the fresh weights even times higher than the negative control. The Best digestate treatment was at a concentration of 80% and a watering frequency of 15 days. In the latter case, the height of the plants and fresh weightwas 65% and 89% greater tan the negative control. In summary, it is posible to improve the growth of lettuce by applying compost or digestate; however, more experiments are needed to find the right combination.

    Time:

    Title: The Effect of Stress on Behavior and Immunity in Wistar Rats

    Sansri Soraya
    University Badji Mokhtar Annaba, Algeria.

    Biography
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    Biography

    Sansri Soraya
    University Badji Mokhtar Annaba, Algeria.



    Abstract
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    Abstract

    Sansri Soraya
    University Badji Mokhtar Annaba, Algeria.

    Stress is a major current problem both in humans and in animals and implement strategies to limit sometimes adverse effects. In addition, exposure to stress causes behavioral and immune disorder in rodents. Experimentally, this modification is based on the intensity and type of exerted stress. The objective of this work is to study the effect of three types of stresses, acute restraint, and predation by separation to assess immune and behavioral changes in the Wistar rat. Comparison between the three types of stresses, Behavioral and adaptive changes in the rat are an attempt to identify the behavioral parameters evaluated from the open fields andmaze. Our results on stressed rats showed the following: Increased anxiety with onset of depression evaluated in tests in an elevated cross maze and open field. Impairment of spatial memory. Disturbance of the immune system cells and humoral response (monocytes and lymphocytes).

    Time:

    Title: Screening, Characterization, and Biocatalytic Capacity of Lipase Producing Wild Yeasts from Makiling Forest Reserve (MFR) Philippines

    Julianne Vilela
    University of the Philippines, Philippines

    Biography
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    Biography

    Julianne Vilela
    University of the Philippines, Philippines

    Ms. Julianne Vilela is a University Researcher and Bioinformatician at the Philippine Genome Center, University of the Philippines. Ms. Vilela extensive training includes: Genome-wide Association Studies (GWAS), Quantitative Genetics and Genomics at Iowa State University, Iowa, USA, and Plant Genome and Bioinformatics Training at Boyce Thompson Institute for Plant Sciences, Cornell University, USA.



    Abstract
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    Abstract

    Julianne Vilela
    University of the Philippines, Philippines

    Lipases (triacylglycerol acylhydrolases, E.C. 3.1.1.3) are enzymes generally used in several industrial applications. However, even with the numerous industrial applications of lipases, there are limited studies aiming to characterize and optimize lipase activity especially that of yeast lipase-producing microorganisms. This work aims to select and identify lipase-producing yeasts isolated from Makiling Forest Reserve (MFR), Philippines and to optimize conditions to maximize lipase production. A total of 144 wild yeasts were tested for their lipase producing potential and strain B1-7 showed the highest lipase activity in both solid and liquid selection media (7.6 EAI and 0.082 U/mL-min activity, respectively). Strain B1-7 was molecularly identified by sequencing the ITS1-5.8S-ITS2 region of the fungal rRNA operon DNA as Cryptococcus flavescens.The optimum conditions for maximum lipase activity (0.66 U/mL-min) of the putative C. flavescens strain obtained using Response Surface Method (Box-Behnken Design) were 5.0 C:N value, pH 6.0 and 0.5% inducer. Lipase activity was significantly affected by the C:N to percent inducer interaction (p= 0.010) and % inducer (p = 0.040). After a 72h fed-batch fermentation experiment, lipase activity was 0.115 U/mL-min. A negative correlation (R2= -0.426) was observed between lipase activity and biomass suggesting that lipase production did not depend on biomass. Moreover, no change in lactose concentration was observed suggesting that it was not used as the primary source of carbon. Hence, we can exploit the potential of producing a new strain for industrial application.

    Time:

    Title: Evaluation of a Panel of CirRNAs Expression as a Novel Potential Biomarker in Hepatocellular Carcinoma

    Kerolos Atalla Shehata
    Armed Forces College of Medicine, Egypt

    Biography
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    Biography

    Kerolos Atalla Shehata
    Armed Forces College of Medicine, Egypt



    Abstract
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    Abstract

    Kerolos Atalla Shehata
    Armed Forces College of Medicine, Egypt

    Background:Circular RNAs are a newly validated type of non-coding RNAs recently found to be deregulated in several human cancers. Accurate and specific non-invasive biomarkers are urgently needed for the diagnosis and prognosis of hepatocellular carcinoma (HCC). Patients and Methods: We performed bioinformatic analysis to retrieve a novel panel of circRNAs potentially relevant in HCC. We examined their expression in the sera of sixty-eight HCC patients, sixty chronic hepatitis C(CHC) patients and 36 healthy controls using RT-qPCR. We examined the performance characteristics of the selected circRNA biomarker panel in comparison to alpha fetoprotein. In addition, we performed cox regression analysis to correlate between their expression levels and patient survival. Results: The circRNA-based biomarker panel (hsa_circ_00156, hsa_circ -000224 and hsa_circ -00520) showed strong biomarker potential with relatively high sensitivities and specificities. The combined panel showed superior performance characteristics to those of AFP. Conclusion: Through our analysis of this preliminary data, we believe that this novel circRNA-based biomarker panel could potentially be used in the diagnosis and prognosis of HCC.

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