International
Biotechnology and Research Conference

April 25-27, 2018 | Rome, Italy

Program Schedule

  • Keynote Speaker

    Time:

    Title

    Title: Type III Polyketide Synthase from Indian Gooseberry: An ideal Candidate for Metabolic Engineering to Develop Novel Nutraceuticals

    E V Soniya
    Rajiv Gandhi Centre for Biotechnology, India.
    Biography
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    Biography

    E V Soniya
    Rajiv Gandhi Centre for Biotechnology, India.

    Soniya was awarded doctorate from the University of Kerala and worked two years at Central Tuber Crops Research Institute, Sreekaryam, Thiruvananthapuram before joining Rajiv Gandhi Centre for Biotechnology. She is the Fellow of National Academy of Science, India. She was the INSA/DFG visiting Scientist in Max Planck Institute of Chemical Ecology, Jena, Germany. She organized the EMBO Conference in Plant Science in RGCB, Thiruvananthapuram. Her area of research is Characterisation of small functional non-coding RNAs and Type III polyketide synthases for exploiting its potential use in metabolic engineering. She has published morethan 20 research articles in peer reviewed International Journals and presented her research works in so many international conferences. She wrote several chapters in the Plant biology field.



    Abstract
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    Abstract

    E V Soniya
    Rajiv Gandhi Centre for Biotechnology, India.

    Emblica officinalis Gaertn (Indian Gooseberry) is one of the most important medicinal plants used in various Ayurvedic formulations and traditional medicine. The plant is native to India, also grows in the tropical and subtropical regions including China, Pakistan, South East Asia and Srilanka. It has beneficial role in cancer, diabetes, liver treatment, anaemia and has application as antioxidant, immunomodulatory, antipyretic, analgesic etc. The plant is rich in secondary metabolites like flavonoids, phenols, tannins, alkaloids and vitamin C. Because of the numerous health benefits attributed to the consumption of E. officinalis as tonic and medicine, there is considerable interest in defining the pathways that control the accumulation of active components. It has been suggested that type III polyketide synthase (PKS) family of enzymes has specific role in the biosynthesis of natural products like flavonoids, alkaloids and curcuminoids. We could successfully isolate and characterise a novel type III polyketide synthase from E. officinalis. Along with this focus is also given to improve the substrate specificity of the identified type III PKS with the aid of site-directed mutagenesis. The findings of the in vitro and in planta functional characterization of the mutants based on high-throughput metabolomics is one of the interesting outcomes of the study. Heterologous expression and in vitro enzymatic assay of EoPKS1 mutants resulted in the formation of quinolone/acridone precursors which can contribute to the large scale combinatorial biosynthesis of pharmaceutically relevant compounds in vitro.

    Sessions:
    Industrial and Microbial Biotechnology

    Time:

    Title: New Opportunities of Biocatalysis in Industrial Biotechnology

    Luis Fonseca
    Institute for Bioengineering and Biosciences, Portugal.

    Biography
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    Biography

    Luis Fonseca
    Institute for Bioengineering and Biosciences, Portugal.

    Luis P. Fonsecais Associate Professor with Habilitation and Ph.D. of Department of Bioengineering at Instituto Superior Tecnico (I.S.T.) of University of Lisbon (U.L.), Portugal and Senior Research Scientist (PI) at the Institute for Bioengineering and Bioscience (iBB) at I.S.T. Prior positions included Chemical Engineer at Hovione, Lda (1985-1986), a Visiting Researcher at CIPAN, Lda (1987-1988), Post-Doc at The School of Biochemistry and Molecular Biology, University of Leeds (1997-1998), Visiting Scholar at Chemical Engineering Department, University of California, Berkeley (2004-2005), and at Institute of Chemistry at University Federal of Rio Grande do Sul, Brazil (2011-2012) and São Carlos at University of São Paulo, Brazil (2015 and 2017).



    Abstract
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    Abstract

    Luis Fonseca
    Institute for Bioengineering and Biosciences, Portugal.

    Chemical products continue to have vital importance to the world economy and are essential to the health, food and consumer product industries.However, theyled to environmental damage and a low public perception of chemical industry. For that reason, there is increasing pressure from both society and Governments for development of new industrial processes to become more sustainable, reducing waste and preventing the use of toxic substances. Consequently, there is a huge opportunity for research and implementation of new cleaner and green processes in the field of industrial biotechnology namely Biocatalysis and Biotransformation. Within this scope, Luis P. Fonseca has focus his research interests on application of biocatalysis in green processes, and development and implementation of reaction media using basically water due to higher enzyme stability and also increasing environment concerns. The design of oil-in-water emulsions (also named miniemulsions) have allowed the production of a high range of high-value products from flavors and fragrances, emollients, polymers, nutraceuticals, and specialty and fine chemicals that led to the development ofAromase technology. This technology is characterized by the enzymatic catalysis, utilization of renewable material resources, high energetic efficiency, no toxicity by using reactions based in water, prevention of waste and sub-products,, among many others advantages. Later modification of the miniemulsion composition evolved to NanoLipCar technology with main goal to design and processing of new drug delivery carriers for encapsulation of cosmeceuticals, nutraceuticals and pharmaceuticals at form of Lipid Nano-Emulsions (LNEs) and Lipid Nano-Particles (LNPs) in this last using long fatty acids that solidify at higher than 37°C.

    Time:

    Title: Potential of Indigenous Lactococci as Starter Cultures in Dairy Industry

    Mirjana Bojanic Rasovic
    University of Montenegro, Montenegro.

    Biography
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    Biography

    Mirjana Bojanic Rasovic
    University of Montenegro, Montenegro.

    Dr. Mirjana Bojanic Rasovic began to work in 1994 at Biotechnical Institute, now Biotecnical faculty in Podgorica. From 2012, she is a senior research associate in the field of Animal hygiene and Preventive Diseases and Microbiology. For two terms (2003 to 2007), she was president of the Society of Microbiologists of Montenegro for which time she organized several scientific meetings and lectures. She has participated in the work of a number of domestic and foreign scientific and professional conferences. As author or co-author, about 80 scientific and professional papers have been published. She was the head of national projects "Influence of zoohygienic conditions on the appearance of mastitis in high dairy cows", 2001-2003., "Examination of the spread of bovine infections with Mycoplasma bovis in the Montenegro",Isolation and characterization of indigenous lactic acid bacteria for the production of specific cheeses in Montenegro", (2009-2012). Dr Mirjana Bojanic Rasovic is Member of following committees: The Veterinary Chamber of Montenegro, Commission for Accreditation of the Accreditation Body of Montenegro, Technical Committee for Laboratories of the Accreditation Body of Montenegro, Sectoral Commission for Agriculture, Food Processing, Committee for Agriculture and Forestry of the Montenegrin Academy of Science and Arts, Commission of the Ministry of Agriculture and Rural Development for professional exam for graduate veterinarians.



    Abstract
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    Abstract

    Mirjana Bojanic Rasovic
    University of Montenegro, Montenegro.

    The diversity of lactic acid bacteria in traditional dairy products represents great potential in biotechnology. In order to standardize indigenous products, the basic requirement is the application of the determined indigenous lactic acid bacteria as starter cultures affecting their specific characteristics by performing fermentation and influencing the ripening process. Starter microorganisms produce lactic acid, which is very important during the coagulation and texturizing of the curd cheese. Production of volatile compounds (e.g. diacetyl and acetaldehyde) contribute to the flavor of these dairy products. Starter culture may posses a proteolytic and lipolytic activity that may be desirable, especially during the maturation of some types of cheese. They also produce bacteriocins, which prevents the growth of pathogens and many spoilage microorganisms. In the process od fermentation of cheese usually participate bacteria of the genus Lactococcus-Lc.lactis ssp. lactis, Lc. lactis ssp. cremoris and homofermentative lactobacilli. Lactococcus lactis species is one of the most important of lactic acid bacteria that are used in the dairy industry. The major functions of this species in dairy fermentation are the production of lactic acid from lactose, hydrolysis of casein and citric acid fermentation. Their metabolic end products and enzymes directly or indirectly have significant influence in determining the texture and flavour of the final products. The utilization of lactococci isolated from indigenous fermented milk products would lead to potential starter cultures with the necessary properties for typical local products that are well accepted by the local population. Also, the use of such starter cultures would allow the production of cheese with designated geographical origin.

    Time:

    Title: Immobilization of ARM Lipase for Industrial Use

    Raja Noor Zaliha Raja Abd Rahman
    University Putra Malaysia, Malaysia

    Biography
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    Biography

    Raja Noor Zaliha Raja Abd Rahman
    University Putra Malaysia, Malaysia

    Raja Noor Zaliha Raja Abd. Rahman obtained her B.Sc. (Hons)-Microbiology from Universiti Sains Malaysia in 1989 and her M.S. in Microbiology from Universiti Putra Malaysia in 1994. She obtained her Doctor of Engineering in Molecular Biology from Kyoto University, Kyoto, Japan in 1998. She is currently the Professor and Head of Enzyme and Microbial Technology Research Centre, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia. In recognition of her scientific findings, she has been awarded patents, prizes and commendations and has derived personal satisfaction from the success of her graduate students, her participation on national and international governmental projects and not least, having a bacterial species named after her.



    Abstract
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    Abstract

    Raja Noor Zaliha Raja Abd Rahman
    University Putra Malaysia, Malaysia

    ARM lipase was isolated from Geobacillus sp. strain ARM, holds many potentials for industrial applications as it is thermostable, organic solvent tolerant, 1,3-regioselective. It prefers medium and long chain fatty acids as substrate. In this study, the enzyme was overexpressed in Escherichia coli system and then purified using affinity chromatography. The purified enzyme was immobilized in gelatinized sago solution and spray-dried by entrapment technique in order to enhance the enzyme operational stability for handling at high temperature and also for storage. The physical characteristic of the immobilized enzyme was studied by scanning electron microscopy, surface area and porosity. The immobilized ARM lipase showed good performance at high temperature. The immobilized enzyme could be stored at for few months without loss of activity. Collectively, the immobilized lipase shows promising capability for industrial use.

    Time:

    Title: Efficient Retting of Natural Fibre Using Microbial Enzyme

    Wan Zuhainis Saad
    Universiti Putra Malaysia, Malaysia.

    Biography
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    Biography

    Wan Zuhainis Saad
    Universiti Putra Malaysia, Malaysia.

    Wan Zuhainis Saad is an associate professor in Microbiology. Research activities include drug discoveries from thermophilic fungi and lactic acid bacteria, microbial enzymes technology in biopolymers and pulp and papers. Awarded three patents on isolation and preservation techniques of thermophilic fungi and enzyme for bioretting of kenaf. An Educational Technology enthusiast. Practices E-learning and active learning approach in enhancing students engagement for effective and meaningful learning. Shortlisted for the QS Wharton Reimagine Education Award 2015 and 2016. QS Wharton Reimagine Education Awards Judge 2017. Awarded Vice-Chancellor Fellowship Award (Young Educator) 2012 and Vice-Chancellor Fellowship Award (Excellent Educator) 2016.



    Abstract
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    Abstract

    Wan Zuhainis Saad
    Universiti Putra Malaysia, Malaysia.

    The global natural fibre composites market is forecast to grow 8.2% from 2015 to 2020. The major driver for the growth of this market is; The rise in demand for lightweight and environmentally sustainable composite materials in various applications, such as automotive, building & construction, and others.In this market, natural fibre (hemp, flax, jute, kenaf, and others) are the major raw materials used for producing natural fibre composites. Kenaf also known as Hibiscus cannabinus is an annual fibre plant closely related to cotton. The kenaf bast fibre found its application in many industries such as textile industry, automotive industries, structural and building materials and made into biocomposite consumer products. The process of separating the bast from the core by degrading the pectin rich middle lamella is known as retting. The traditional method of water retting requires a longer retting time and caused pollution while dew retting produced fibres with poor quality. Hence, there is a need to seek for an environmental-friendly approach to produce high-quality kenaf bastfibres. In this study, pectinolytic fungi were isolated from various sources and screened for their pectinase activity. A potential pectinase producing strain was chosen and identified as Aspergillus fumigatus R6 by amplification of the Internal Transcribed Spacer region. It was found the retting process using the enzyme has reduced the retting time from 3 weeks to 3 days. Kenaf bast fibres quality can improve by further optimisation of the enzyme formulation. Aspergillus fumigatus R6 pectinase enzyme showspotential to be used in kenaf bast bio retting process to produce strong and high quality kenaf long bast fibres.

    Time:

    Title: Purification of α-Amylase from Thermophilic Bacillus Licheniformis and its Application in the Production of Ethanol

    Reena Gupta
    Himachal Pradesh University, India

    Biography
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    Biography

    Reena Gupta
    Himachal Pradesh University, India

    Presently working as Professor in the Department of Biotechnology, Himachal Pradesh University, Summerhill, Shimla. Area of specialization is Biochemistry, Immunology and Enzyme Technology. Completed B.Sc. (Hons.) and M.Sc. (Hons.) in Biochemistry from Panjab University, Chandigarh in the year 1985 and 1987 respectively. Ph.D. was completed in the year 1992 from Panjab Unversity, Chandigarh and Post Doctorate from May 1993 to Oct. 1995 from IMTECH (Institute of Microbial Technology), Chandigarh. She has a 22 years of teaching and 29 years of research experience. She completed four Research Projects funded by UGC, New Delhi and one Project funded by DEST, Shimla is ongoing. The amount of total fund generated through Projects is 20.54 lakhs. Total number of publications in peer reviewed Journals of international repute are 87 (National and International). She is a Member of five National Professional Bodies.



    Abstract
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    Abstract

    Reena Gupta
    Himachal Pradesh University, India

    An a-amylase from thermophile Bacillus licheniformis was purified by using DEAE- Cellulose column chromatography followed by Sephadex G-75 column chromatography. Native and SDS-PAGE analyses showed a single band for the purified enzyme, with an apparent molecular weight of 33 kDa. This showed that the enzyme was purified to homogeneity, was a monomer and had no subunits. The α-amylase immobilized on WhatmanTM filter paper showed maximum activity at 55°C of incubation temperature, 10 min of incubation time and pH 8.5 of 0.1M Tris-HCl buffer. The immobilized enzyme gave maximum activity with 0.175% (w/v) of substrate (starch) concentration and showed Km and Vmax value of 0.74 mM and 6.39 μmol/g/min respectively. Immobilized enzyme retained almost 50.25% of its original activity after 9th cycle of reusability. During storage, immobilized enzyme retained relative activity of 90.87% and 56.28% upto 10 and 40 days respectively. The immobilized enzyme produced maximum amount of reducing sugar from maize stalk juice extract when incubated at 55°C of temperature at pH 8.5 and for 20 min of incubation. It was observed that there was a 50-fold increase in production of ethanol from extract of maize stalk treated with immobilized α-amylase (27.33%) as compared to the untreated sample (18.92%).

    Time:

    Title: Effect of Clarifying Agents on the Volatile Composition of Mead

    Leticia M. Estevinho
    Instituto Politecnico de Braganca, Portugal.

    Biography
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    Biography

    Leticia M. Estevinho
    Instituto Politecnico de Braganca, Portugal.

    Leticia M. Estevinho received the Zootechnical Engineering degree in 1985, the MS Degree in Biotechnology in 1989 and the PhD Degree in Science in 1995. In 2009 passed with merit the proofs of “Agregacao”. Leticia M. Estevinho published more than 100 articles in indexed international journals, wrote several book chapters and presented more 160 oral and written communications. Leticia Estevinho is the Head of the Microbiology Laboratory of Escola Superior Agraria de Braganca since 1986 and has been the principal investigator of more than 30 financed research projects.



    Abstract
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    Abstract

    Leticia M. Estevinho
    Instituto Politecnico de Braganca, Portugal.

    Mead is an ancient alcoholic beverage containing between 8% and 18% alcohol by volume. It is obtained by fermentation of honey-wort though a complex process demanding both long-term fermentation and maturation. In wine production different procedures are applied for organoleptic properties’ stabilization and improvement, among which clarification. However, studies regarding those procedures in the context of mead fermentation are practically non-existent. This study aimed to assess the effect of several clarifying agents (i. casein, ii. gelatin, iii. silica, iv. bentonite, v. tannins and vi. bentonite + gelatine + egg yolk) on the volatile composition of mead. The volatile compounds were determined by gas chromatography fitted with a flame ionisation detector (FID) and by gas chromatography-mass spectrometry (GC-MS). Thirty-six volatile compounds were identified, among which 42.50% belong to the group of alcohols, 40.40% were carbonyl compounds, 14.40% were acetates and 1.8% were esters. Volatile compounds’ concentration differed according to the concentration and type of fining agent used. Significant differences (p < 0.05) were found in ten volatile compounds independently of the type of treatment used. Highest volatile compounds’ concentration, mainly esters, lactones, terpenes and norisopenoids, were observed in meads clarified with silica; the lowest values were obtained for samples in which tanines were used. From the identified compounds, eleven had major impact on meads’ aroma, with OAV>1. These results contribute to select the fining agent to be used in mead fermentation and, to a later extent, to improve the sensorial characteristics of this beverage.

    Time:

    Title: Production and Evaluation of Protease Enzyme (B.subtilis strain) for Eco-friendly Leather Processing Empowerment in Ethiopian Tanneries

    Biruk Abate
    Ku Lueven Engineering College, Belgium.

    Biography
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    Biography

    Biruk Abate
    Ku Lueven Engineering College, Belgium.

    Biruk Abate Fenta is Lecturer and researcher at Bahir Dar Institute of Technology, Bahir Dar University in the Faculty of Chemical and Food Engineering and Biotechnology Research Institute. He received a B.S. degree in Chemical Engineering from Bahir Dar Institute of Technology, Bahir Dar University, Ethiopia and the Master of Technology in Chemical Engineering from the Addis Ababa Institute of Technology, Addis Ababa University, Ethiopia. He has been active in the area of Postharvest and Energy technologies, water quality and environment, chemicals and Pharmaceuticals researches in addition to his regular work of lecturing undergraduate students and mentoring postgraduate students during their thesis work. His national experience includes consulting feasibility project works of household/small scale, medium and large scale industries across his country. Currently he is an international PhD student in Ku Leuven Engineering Technology, Leuven, Belgium



    Abstract
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    Abstract

    Biruk Abate
    Ku Lueven Engineering College, Belgium.

    Across the world the tanning industries are facing a high challenge pressure from strict legislation articulated for the purpose of protecting and preserving the environment. In Ethiopia leather processing industry is considered from the directions of economic activity and environment polluting concern. In order to operate it within the environmentally compatible situations and sustain the agriculture development led industrialization policy and the ongoing development strategy of the tanning industry in Ethiopia should come out that can mitigate the adverse effect of the conventional leather processing methods particularly that of lime-sulphide dehairing in beam house operation. This study was focused on dehairing enzyme production as green technology alternative for the existed conventional unhairing practice. The B.subtilis strain was produced from microorganism from beam house waste from the local leather processing areas, sub-cultured and characterized for its growth and dehairing protease production in terms of pH, temperature, incubation time and growth and production media composition. The combined effects of pH and temperature on protease production also investigated and they were found to have high interactive effect. After the culture conditions for production were clearly studied the alkaline protease was produced in a laboratory scale fermenter containing ingredients with required concentration of glucose , peptone,MgSO4.7 H2O, KH2PO4 and FeSO4.7H2O and it was maintained at a temperature about 37°C for 24 to 48 hoursand trembled uncontrollably in the incubator operated close to 140rpm. After completion of fermentation time all fermented sauce base of the broth was separated by centrifuge rotated at 10,000 rpm at 4°C for 15-18 min and the clear liquid part was taken for crude enzyme preparation. From this clear supernatant liquid part the concentration of protease enzyme produced in the production medium was analyzed at best conditions near to PH 8.5, temperature 37°C and incubation time of 24 hours and closer to the 96 hrs operations maximum amunt of enzyme was yielded. The final protease product was recovered, partially purified and stabilized by primary downstream processing such as crude enzyme formulation, ammonium sulphate precipitation. The use of the protease product of this technique on sheep skins resulted in a highly promising hair removal efficiency that can really compete with lime-sulphide chemical unhairing process.

    Time:

    Title: Mathematical Modeling of the Anaerobic Digestion in Two-Stage System with Production of Hydrogen and Methane

    Ivan Simeonov
    Bulgarian Academy of Sciences, Bulgaria

    Biography
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    Biography

    Ivan Simeonov
    Bulgarian Academy of Sciences, Bulgaria

    Present job: Head of research group “Mathematical modeling and computer sciences” Professional Experience: Scientific research, education and applications in Mathematical modeling and Automatic Control of biotechnological processes and especially of the anaerobic digestion of organic wastes; Invited Professor in University of Science and Technology (Nanjing, Chaina), University of Lille (France), University Paris-Sud 11 (Paris, France), University of Turku (Finland), University of Marseille, (France). Publications: more than 250 scientific (4 books) and 20 popular papers in Bulgarian and International Journals, Conferences and Congresses and more than 450 citations in different journals and conferences.



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    Abstract

    Ivan Simeonov
    Bulgarian Academy of Sciences, Bulgaria

    Anaerobic digestion (AD) is a multi-step biotechnological process with hydrogen (H2) as an intermediate product. In order to completely utilize the organic acids produced and improve the overall energy conversion efficiency, a two-stage AD concept consisting of hydrogenic process followed by methanogenic process has been suggested. In this paper two mathematical models of a continuous process of AD with production of hydrogen and methane in a cascade of two bioreactors (BR), were developed and comparatively studied. They correspond of the following two cases: 1. Modeling of the hydrogen production in the first stage (BR1) on the base of dynamic balance of the substrate, biomass and product (acetate) formation. Modeling of the methane production in the second stage (BR2) of the process on the base of the acetate produced in the BR1 by the methanogenic population (simplified model). 2. Modeling of the hydrogen production in BR1 on the base of the dynamic balance of the effluent substrate (cellulose), obtained cellobiose (after hydrolysis), biomass and intermediate products (acetate, propionate and butyrate). Modeling of the methane production in BR2 on the base of the acetate, propionate and butyrate produced in the BR1 by propionate degrading, butirate degrading and methanogenic populations (more complicated model). It was proved that in both cases the static characteristics present maxima of the obtained biogas (containing H2 or CH4) in the admissible range of the control input and that defines the optimal ratio of the working volumes of the bioreactors. Simulation studies illustrated the feasibility of this scheme.

    Time:

    Title: Sustainable and Efficient Recovery of Polyhydroxyalkanoate Polymer from Cupriavidus Necator Using Environment Friendly Solvents

    Geeta Gahlawat
    Panjab University, India

    Biography
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    Biography

    Geeta Gahlawat
    Panjab University, India

    Dr. Geeta Gahlawat is presently working as UGC-Kothari Postdoctoral fellow at Panjab University, India. She obtained his Ph.D. degree in November 2014 from Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, India. During PhD, she has worked on the project entitled "Microbial production of Polyhydroxybutyrate (PHB) and its copolymers". She has more than 8 years of experience in fermentation and microbial technology along with bioreactor operation and their maintenance. She has systematically built a firm foundation of bioprocess engineering fundamentals to optimize any fermentation processes. She has published eight papers in peer reviewed International Journals.



    Abstract
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    Abstract

    Geeta Gahlawat
    Panjab University, India

    An imprudent use of environmentally hazardous petrochemical-based plastics and limited availability of fossil fuels have provoked research interests towards production of biodegradable plastics - polyhydroxyalkanoate (PHAs). However, the industrial application of PHAs based products is primarily restricted by their high cost of recovery and extraction protocols. Moreover, solvents used for the extraction and purification are toxic and volatile which causes adverse environmental hazards. Development of efficient downstream recovery strategies along with utilization of non-toxic solvents will accelerate their commercialization. In this study, various extraction strategies were designed for sustainable and cost-effective recovery of PHAs from Cupriavidus necator using non-toxic environment friendly solvents viz. 1,2-propylene carbonate, ethyl acetate, isoamyl alcohol, butyl acetate. The effect of incubation time i.e. 10, 30 and 50 min and temperature i.e. 60, 80, 100, 120°C was tested to identify the most suitable solvent. PHAs extraction using a recyclable solvent, 1,2 propylene carbonate, showed the highest recovery yield (90%) and purity (93%) at 120°C and 30 min incubation. Ethyl acetate showed the better capacity to recover PHAs from cells than butyl acetate. Extraction with ethyl acetate exhibited high recovery yield and purity of 96% and 92%, respectively at 100°C. Effect of non-toxic surfactant such as linear alkylbenzene sulfonic acid (LAS) was also studied at 40, 60 and 80°C, and detergent pH range of 3.0, 5.0, 7.0 and 9.0 for the extraction of PHAs from the cells. LAS gave highest yield of 86% and purity of 88% at temperature 80°C and 5.0 pH.

    Time:

    Title: Enhanced Production, Purification and Thermodynamic Characterization of Endotoxin Free Antileukemic Recombinant L-Asparaginase of Escherichia Coli K-12

    Santosh Kumar Jha
    BITS, India

    Biography
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    Biography

    Santosh Kumar Jha
    BITS, India

    Dr. Santosh Kumar Jha is an academician cum researcher and presently serving as an Assistant Professor in the Birla Institute of Technology, Mesra, India. He has expertise in the process development and designing of bioprocess for the production of biotherapeutics. Presently he is working on the bioprocess development of various biotherapeutics, development of nanobiocomposite based nanobiotics and tissues scaffolds for various biomedical applications.



    Abstract
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    Abstract

    Santosh Kumar Jha
    BITS, India

    L-Asparaginase (E.C. 3.5.1.1) is a well accepted chemotherapeutic agent against the acute lymphoblastic leukemia and lymphosarcoma. The recombinant L-asparaginase enzyme was produced by the over-expression of ansB gene of E. coli K-12 in E. coli BL21. Different carbon sources, nitrogen sources, minerals and additives having yield enhancing effect, were screened by Plackett Burman Design (PBD). Their optimum level was identified by the using the orthogonal array method of Taguchi design of experiment. The screening of media components by PBD proposed the glucose as main carbon source, tryptone and yeast extract as organic nitrogen source, NH4Cl as inorganic nitrogen source, NaCl and K¬2HPO4 as mineral source have significant effect on the production of enzyme. Glycerol was identified as the most influential effect on the recombinant L-asparaginase production among the all additives. The addition of small amount (0.6% v/v) of it, significantly increased the enzyme yield. After the complete optimization of the selected process parameters 121.8% enhanced production of L-asparaginase was observed at shake flask level. There was further 14.8% enhancement in the enzyme production after the scale up the process in 5L bioreactor. The volumetric yield of 3.58 X 105 U/L of L-asparaginase with the specific activity of 6.97 X103 U/mg in fermentation broth was reported. The enzyme was purified by affinity chromatography followed by three-phase partitioning. The endotoxin level was estimated by LAL-assay. The purified recombinant enzyme was further used to study the thermodynamic parameters. The enzyme showed highest stability at 280C than at higher temperatures with a half-life of 46 hrs which is quite significant. Its deactivation energy was found to be 60.64 kJ/mol. The value of thermodynamic parameters including ∆H, ∆S and ∆G were found to be -49.23kJ/mol, 0.09kJ/mol.K and 73.12-74.78 kJ/mol respectively implying that there are no significant processes of aggregation and the enzymatic reaction was exothermic and spontaneous in nature. The antileukemic potential of the enzyme was accessed by using human leukaemia cell line HL-60 through viability study, cell and morphological studies.

    Time:

    Title: Process for Recovering Biomolecules from Fermentation Broth

    Pavithra M K S
    Bannari Amman Institute of Technology, India

    Biography
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    Biography

    Pavithra M K S
    Bannari Amman Institute of Technology, India



    Abstract
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    Abstract

    Pavithra M K S
    Bannari Amman Institute of Technology, India

    A simple, accurate, cost effective and precise method of reverse phase high performance preparative liquid chromatography was developed for maximum recovery and yield of Immunograf and to bring down the impurity level of RRT (0.94) and RRT (1.2) less than 0.5%, the two impurities that affect the assay of the product. For the high performance preparative liquid chromatography, Kromacil-C8, 13 µm column consisting of 10*250mm I.D. was used. The flow rate was 3.925 ml/min and eluent was monitored at 210 nm. In isocratic mode, mobile phase containing KH2PO4 Buffer: Acetonitrile: Butanol of varying ratios was used for the resolution study. The retention time was analysed and gradient run was carried out for the mobile phase ratio that gives maximum resolution. The gradient run with the gradient time of 5, 50, 100, 150, 200 min was carried out for two different trials with mobile phase in two different lanes. Loading trials were carried out for the trials which gave maximum resolution. The samples were analysed and the yield on load was calculated for all the runs. The gradient run with gradient time of 200 mins and mobile phase ratio 22.2:77.8 to 28.8:71.2 Acetonitrile:Butanol:Buffer gave 82% yield on the basis of load, which was found to be maximum among all the trials carried out. Adsorption isotherm parameters were determined for the same.

    Sessions:
    Biosensors and Biomarkers

    Time:

    Title: QCM-Based Biosensors for the Detection of Tumor Released Exosomes

    Agnese Magnani
    University of Siena, Italy

    Biography
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    Biography

    Agnese Magnani
    University of Siena, Italy

    Agnese Magnani is a Professor of Inorganic Chemistry- University of Siena,Department of Biotechnology, Chemistry and Pharmacy. Her research topics are: - Functional polymers, materials and coatings for biomedical and agriculture applications - Biosensors for biomarkersidentification and determination in body fluids - Nanocarriers for drug target delivery - Study of molecular recognition processes: protein-ligand interactions; protein and cell interaction with solid surfaces; biofilm formation - Application of IR and ToF-SIMS to materials and biological systems: thin films, SAMs and nanomaterialscharacterisation; geographical characterisation of agrifood products; development of micro-imaging methods for biological processes in tissues and cells.



    Abstract
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    Abstract

    Agnese Magnani
    University of Siena, Italy

    Biosensors cansatisfy the rapidityand accuracydiagnosisrequirements in cancerbiomarkers(tumorassociatedantigens) detectionduringearlystages of the disease, thusovercomingmany of the problemsrelated to the classicaldiagnosticmethods more expensive and often time consuming. Modernbioaffinitysensors, suchas DNA- or immunosensors, haverecentlydemonstratedgreatpotential for monitoringcancer-relatedproteinmarkers and DNA mutations. Mass-sensitive devices like Quartz Crystal Microbalances (QCMs) are commonly used to develop bio-affinity sensors: this kind of sensor is usually made of an AT-cut quartz disk with electrodes on both sides, one of which is functionalized with a receptor selective to the target analyte. If the quartz is used as a feedback element in an electronic sinusoidal oscillator (setting in this way the oscillation frequency), in case of a bio-recognition event, the change of the mass of the quartz is proportionally converted to a frequency shift of the oscillator frequency, providing an indirect measure of the adsorbed mass with a good sensitivity (1Hz/ng for 10 MHz AT-cut quartzes). The development and the characterization of QCM biosensors for the detection of exosomes is presented. Exosomes are cell-derived vesicles that are present in many biological fluids, and that possess diagnostic potential in the oncologic field. From tests with physiological solutions and human plasma, the developed biosensors have proved to give a rapid response (within minutes) with high sensitivity and specificity against the PSMA (prostate-specific membrane antigen).

    Time:

    Title: Search for Regions with Periodicity Using the Random Position Weight Matrices in Different Genomes

    Eugene Korotkov
    National Research Nuclear University, Russia.

    Biography
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    Biography

    Eugene Korotkov
    National Research Nuclear University, Russia.

    Eugene V. Korotkov is a professor at the Department of Applied Mathematics in Moscow Engineering Physics Institute and Principal Investigator in Bioinformatics Department of Bioengineering Centre, Russian Academy of Sciences. He graduated from the National Nuclear Research University (MEPI), Department of Experimental and Theoretical Physics in 1974. He completed PhD thesis in radiobiology. Then, from 1980 to 1995 he worked at the Institute of Chemical Physics, NN Semenov and since 1995, he is working with the Center ‘Bioengineering’, Russian Academy of Sciences. His research interests are in developing new methods for the analysis of symbolical sequences and time series.



    Abstract
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    Abstract

    Eugene Korotkov
    National Research Nuclear University, Russia.

    The present study developed a mathematical method for determining tandem repeats in a DNA sequence. A multiple alignment of periods was calculated by direct optimization of the position-weight matrix (PWM) without using the pairwise alignments or searching for similarity between periods. A new mathematical algorithm for periodicity search was developed using the random PWMs. The developed algorithm was applied in analyzing the DNA sequences of the different genome. A total of more than 60x103 regions were found to possess a periodicity with the length of 2 to 50 bases. On the average, a periodicity of ~5000 nucleotides was found to be associated with each region. A significant portion of the revealed regions possess periods consisting of 10 and 11 nucleotides, multiple of 10 nucleotides and periods in the vicinity of 35 nucleotides. Only ~30% of the periods found were discovered previously. This study discussed the origin of periodicity with insertions and deletions.

    Time:

    Title: Performance of Microbial Desalination Cell in High Organic Load

    Surajbhan Sevda
    Indian Institute of Technology Guwahati,India.

    Biography
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    Biography

    Surajbhan Sevda
    Indian Institute of Technology Guwahati,India.



    Abstract
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    Abstract

    Surajbhan Sevda
    Indian Institute of Technology Guwahati,India.

    Microbial desalination cell presents a novel concept for wastewater treatment, salt removal and bioelectricity generation in a single system. In this study, the effect of organic load on salt removal in air cathode up-flow microbial desalination cell was investigated in batch mode. Four different organic concentrations were fed to the anodic chamber of microbial desalination cell. The initial chemical oxygen demand in the anodic chamber were 2.02, 4.05, 6.07 and 8.10g/L. A real seawater was used in the desalination chamber of UMDC. The obtained results showed that the organic load 3 (initial COD of 6.07 g/L) produced a maximum percentage of salt removal compared to other organic loads tested. The maximum power density produced (1.77 W/m2), the maximum percentage COD removal (95%) occurred with 48 % salt removal in the microbial desalination cell using the organic load of 6.10mg/L. The obtained results shows that microbial desalination cell is a unique system that provide wastewater treatment and salt removal in a single system. Organic load is a very important factor for the operation of UMDC, there is an optimum value that achieve the best performance of the UMDC system.

    Sessions:
    Biotechnology in Agriculture

    Time:

    Title: Towards the Development of a Multidimensional Multisensor Spatiotemporal Model for Disease Detection and Spread

    Maria Regina Justina Estuar
    Ateneo de Manila University, Philippines

    Biography
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    Biography

    Maria Regina Justina Estuar
    Ateneo de Manila University, Philippines

    Maria Regina is a Fulltime Professor at the Department of Information Systems and Computer Science, Ateneo de Manila University. She currently manages two laboratories: Ateneo Java Wireless Competency Center and the Ateneo Social Computing Science Laboratory where research focus is on in the design and development of social, mobile and wireless systems to understand and model collective behavior and capacity. She teaches Social Computing, Information Systems for Disaster and Health.



    Abstract
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    Abstract

    Maria Regina Justina Estuar
    Ateneo de Manila University, Philippines

    Conductivity, amount of light, and air temperature. Weather data include air temperature, relative humidity, rainfall, and air pressure. Mobile phone data include leaf images captured from infected and non-infected sites. Weather data is used to impute for sWith the increasing availability of low cost devices in monitoring crops, small scale farmers, especially in developing countries, now have the opportunity to access Smart farming technologies in monitoring crops. The Philippines is the 3rd largest export producer of Cavendish banana. There are also over 180 small to medium scale banana plantations, nationwide. This study attempts to develop a predictive model for the the early detection of Fusarium oxysporum f. sp. cubense Tropical Race 4 or more commonly known as Panama Wilt, a soil born fungi based disease that greatly affects the production of bananas in the country. The study attempts to test whether a multidimensional approach, using multi-sensor data can be used to develop a geospatial predictive model for early detection of disease. Data is extracted from three sources including soil sensors, weather station, and mobile phone. Soil data include data coming from home grown pH sensors calibrated to work with an off the shelf soil sensor that captures soil temperature, soil moisture, soil data that was corrupted or not captured for some time period. A resulting ARIMAX model has been created that had an RMSE of roughly 16 epidemic incidences for short-term forecasting. Image data has also been explored to generate structural features of possibly infected banana leaves through an autoencoder, which allows the feature extraction for a single class. This results to latent variables that efficiently encode significant information in the images. The success rate of the autoencoder is at 91% for healthy specimens, while only at 78% for infected samples. Integrating the various data extracted from both time-series and image-based models, a linear model that incorporates both spatial and temporal factors can be created to quantify and predict disease spread.

    Time:

    Title: A Framework for Real time Detection and Monitoring of Plant Diseases: A Case Study on Use of Web and Mobile Applications on Foc TR4

    Marlene M. De Leon
    Ateneo de Manila University, Philippines

    Biography
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    Biography

    Marlene M. De Leon
    Ateneo de Manila University, Philippines

    Marlene De Leon earned her Ph.D. in Computer Science degree from the Ateneo de Manila University, Philippines. She currently works as a full-time Associate Professor at the Department of Information Systems and Computer Science (DISCS), where she teaches Software Engineering, Systems Analysis and Design, and Database Management. She serves as one of the mentors in the Ateneo Social Computing Science Laboratory and the Ateneo Java Wireless Competency Center where disaster management systems and healthcare management systems are developed.



    Abstract
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    Abstract

    Marlene M. De Leon
    Ateneo de Manila University, Philippines

    The proliferation of Internet of Things (IoT) devices and cyberphysical structures allowed mobile and web applications to be used for early detection and monitoring of plant diseases. The Cloud-based Intelligent Total Analysis System (CITAS) is an intelligent farming system composed of mobile applications, web applications, and wireless sensor networks (WSNs). It aims to detect early and monitor the presence of Fusarium oxysporum cubense TR4 (Foc TR4) on Cavendish bananas cultivated in the Philippines using modeling and analysis. CITAS Mobile was developed as a data collection tool for farmers. Data collected include: 1) plant leaf images; 2) qualitative plant characteristics; 3) soil WSN parameter readings (e.g., humidity, pH); and 4) GPS coordinates. CITAS Mobile analyzes plant leaf images in real-time to determine the presence or absence of the disease. Data collected by the mobile application are sent to the CITAS Server through its Application Program Interface (API) end-point via mobile internet connection for multidimensional modeling and analysis. CITAS Web provides farm owners and researchers access to historical data collected by CITAS mobile. CITAS Web has the following features: 1) data-entry of soil sample characteristics; 2) database display of farms and plants; 3) historical trend visualization of soil WSN parameter readings; and 4) visualization of multidimensional analysis results involving plant leaf images, microscopic soil images, soil WSN parameter readings, and soil physico-chemical and nutrient characteristics. CITAS provides a framework for collection, management, analysis, and visualization of plant disease spread in a geospatial interface. Currently, total analysis allows for early detection and monitoring of Foc TR4. Farmers, farm owners, and researchers may use CITAS for early detection and monitoring of other plant diseases through extension of its analysis parameters. Through this study, the framework can be modified and used to monitor other plant diseases.

    Time:

    Title: The Automated Detection of Fusarium Oxysporum Sp. in Soil Samples through Mapped Methods of Image Analysis and Machine Learning

    Andrei D. Coronel
    Ateneo de Manila University, Philippines

    Biography
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    Biography

    Andrei D. Coronel
    Ateneo de Manila University, Philippines

    Dr. Andrei Coronel graduated with Bachelor of Science degree in Biology in 1999, a Master of Science degree in 2005, and a Ph.D. degree in Computer Science in 2014. He was a Research Engineer for the IIR(A-Star)/NUS in Singapore last 2008, where he contributed in the development of an ontology-based cancer database system. He involves himself with projects that intersect CS with health or environmental science, such as this recent project in UC Berkeley. He is currently the head of the Ateneo Computational Sound and Music Lab in his University, furthering his research oriented in data analytics.



    Abstract
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    Abstract

    Andrei D. Coronel
    Ateneo de Manila University, Philippines

    The export of bananas is a major area of trade in the Philippines, with Cavendish cultivars making up 50% of the export volume of this market. This type of banana, however, is susceptible to the infection of Fusarium oxysporum sp. Cubense, Tropical Race 4 (Foc TR4), the fungus that causes Fusarium Wilt. Capable of rendering wide areas of banana farms with infected plantations, Foc is a significant threat to this commerce. There is therefore a need for the early detection of Foc, especially in juvenile and asymptomatic bananas. This study has implemented an image analysis and machine learning approach in detecting Foc in magnified soil samples. Several image analysis methods were tested alongside a number of machine learning techniques towards the goal of identifying an ideal mapping of these procedures. This study not only served to improve the classification accuracy of Foc identification, but the results also acted as a guide to the development of both the soil preparation protocol and mobile microscope design. The results show that CNN, ANN, and SVM machine learning methods yield classification accuracies of 72.6%, 82.23%, and 81.12%, respectively, where SVM performed with the fastest processing time. It is conclusive that a fluorescence-based soil preparation protocol paired with at least 100x magnification and a phase contrast modality produces a viable input for a shape-recognition based image analysis technique. The study is an important step towards a multi-parameter approach in the early detection of Foc TR4 infection, thus potentially changing farmer behavior from reactive practices to preventive measures in the context of Fusarium Wilt. Similar methods can be applied in other plant diseases that make use of image analysis for early detection of diseases.

    Time:

    Title: Utilizing Induced Mutagenesis In Kazakhstani Wheat Breeding

    Zhanar Chunetova
    Al-Farabi Kazakh National University, Kazikisthan.

    Biography
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    Biography

    Zhanar Chunetova
    Al-Farabi Kazakh National University, Kazikisthan.



    Abstract
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    Abstract

    Zhanar Chunetova
    Al-Farabi Kazakh National University, Kazikisthan.

    Mutagenic effect of physical factors and chemical substances (aziridine or ethylene imine, nitrosoethylurea, nitrosoethyleneurea) leads to increase of the spectrum of hereditary variability for breeding purposes, which however is not studied in full extent. Ecological study of anthropogenic factors action leading to disruption of certain links between chemical elements and their combinations, raise of heavy metals concentration in soil, facilitate examination of mutagenic and toxic properties of heavy metals. Increase in wheat yields by improving its genotype is one of the most urgent problems of agriculture and economy. Atpresent, using traditional methods of selection and genetic studies, such as backcross selection, distant hybridization, and experimental mutagenesis, increased efficiency of obtaining genetically modified and improved forms of wheat. Heavy metals are defined as metals having a density higher than 5 g/cm3. Of the total 90 naturally occurring elements divided into three classes by the degree of their threat, 53 are considered heavy metals and few are of biological importance. Accumulation of heavy metals such as cadmium (Cd) in the environment is now becoming a major cause of environmental pollution. Toxic metals can inactivate proteins, shifting metal cofactors, blocking active centers or causing allosteric changes. Besides, large number of those possesses ability of inducing mutagenic changes, tumors and causing macroscopic changes. Molecular mechanism of heavy metals toxicity is not completely understood. Cd is non-essential element that negatively affects plant growth and development, released into the environment by power stations, heating systems, metal working industries or urban traffic, which has high cumulative effect with almost no biodegradation. In plants it affects such processes as stomata opening, transpiration and photosynthesis, consequently chlorosis, leaf rolls and stunting are the main symptoms of Cd toxicity in plants accompanied by root browning, leaf red-brownish discoloration. It can also reduce the absorption of nitrate from root to shoot by inhibiting the nitrate reductase activity in shoots. The negative effect of Cd on plant growth was accompanied by an increase in dry to fresh mass ratio in all organs. Several researches have suggested that an oxidative stress could be involved in cadmium toxicity, by either inducing oxygen free radical production, or by decreasing enzymatic and non-enzymatic antioxidants. On the other hand, the use of induced mutagenesis showed high efficiency in the production of forms with high yield, quality bakery, lodging resistance, modified plant height and resistance. And, this paper is an attempt of summarizing results performed by our group in this direction.

    Time:

    Title: Plant Regeneration from Immature Embryos of Foxtail Millet: A Millet and Biofuel Crop

    Kokiladevi Eswaran
    Agriculture College Research Institute, India.

    Biography
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    Biography

    Kokiladevi Eswaran
    Agriculture College Research Institute, India.



    Abstract
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    Abstract

    Kokiladevi Eswaran
    Agriculture College Research Institute, India.

    The small millets comprising six species, namely, finger millet (Eleusinecoracana), little millet (Panicumsumatrense), Italian or foxtail millet (Setariaitalica), barnyard millet (Echinochloacrusgalli), proso millet (Panicummiliaceum) and kodo millet (Paspalumscrobiculatum) are grown in about 2 million ha area in India, Among these, finger millet is the most important and occupies about 60% of the area and contributes 70 % of small millet production. These crops are hardy and quite resilient to varied agroclimatic adversities and play important role in marginal agriculture more common in hilly and semi-arid regions as important source of food grain as well as highly valued fodder. Many kinds of traditional foods and beverages are made from these grains in different regions and hence have important role in the local food culture. Nutritionally, they have high micronutrient content, particularly calcium and iron, high dietary fibre, higher amount of essential amino acids and low glycemic index and thus play an important role in the food and nutritional security of the poor. However, their presence in the Indian food basket had been declining over the years primarily due to wheat and rice being available at subsidized rates. These species are neglected in research and development and are not receiving the policy support they need and rightly deserve. This neglect is also causing the marginalization of farmers who have been traditionally depending on these crops for their food security and income. However, there is an increasing recognition of their favourable nutrient composition and utility as health food, in the context of increasing life style diseases. Thus, apart from their traditional role as a staple for the poor in the marginal agricultural regions, they are gaining a new role as crops for healthy food and for the urban high income people. To meet the strong increase in cereal demand worldwide, new approaches and technologies for generating new varieties are necessary. One of these methods is the creation of transgenic plants with desirable traits. Although millets are economically important, especially in the developing world, little genetic improvement has been done so far specifically using wide- or cross- hybridization among closely related species. The incompatibilities due to interspecific hybridization are alleviated by directly transferring the desirable traits to millets using optimum or efficient transformation method. Hence, crossing barriers could be overcome, and genes from unrelated sources would be introduced asexually into crop plants. Monocots in general and cereals in specific were initially difficult to genetically engineer, mainly due to their recalcitrance to in vitro regeneration and their resistance to Agrobacterium-mediated infection. However, efficient transformation protocols have been later established for the major cereals including rice and maize. Gene transfer to millets would be facilitated once efficient or optimum regeneration and transformation techniques are established. The optimization of regeneration method is, therefore, necessary for different millet types in order to increase the efficiency of transformation. Hence, a study was taken in foxtail millet to develop tissue culture regeneration protocol for high frequency shoot regeneration from immature embryos through somatic embryogenesis. In this study,we report optimization of the regeneration system for foxtail millet through improvement of the regeneration system efficiency. Immature inflorescences explants of foxtail millet cv. Co (Te)7 varying in length (0.5 to 1.0 cm) were cultured on modified MS medium for callus induction and regeneration. Calli were obtained at the highest frequency (80%) from immature inflorescences on MS containing 2, 4 D (2.5mg/L). The efficiency declined dramatically namely 40, 60 and 60% in MS medium containing 2, 4, D 1.0, 2.0 & 3.0 mg/L. The results indicate that 2, 4, D (2.5mg/L) is the best concentration for callus induction. To assess the regeneration potential, calli induced from MS medium containing 2, 4, D (2.5mg/L) were transferred to regeneration medium with different concentrations of BAP. Differentiation was defined as the development of green spots distributed discretely throughout the callus within two weeks of transfer. The differentiation rate (percentage of differentiated calli among the total number of embryogeniccalli) was varying. The calli induced from 2,4 D (2.0 mg/L) and cultured for 25 days in regeneration media containing BAP (1.5 mg/L) showed the highest differentiation frequency. The differentiation frequency decreased with increasing concentration of BAP.Shoots were developed from the green spots after one-month culture on regeneration medium and the number of shoots per callus varied from one to ten. Shortly after, the shoots (about 1 cm long) were excised and transferred to rooting medium (1/2MS + 1 mg/l L-Proline + 800 mg/l Casein Hydrolysate + 3 % (w/v) Sucrose + 0.3 % (w/v) Phytagel, pH 5.8) for two weeks until roots developed. Regenerated plantlets with fully grown shoots and roots were gradually acclimatized in the greenhouse and the survival rate was more than 90%. These results demonstrate that calli induced from 0.5 to 1.0 cm immature inflorescences in MS containing 2, 4 D (2.5 mg/L) is optimal for regeneration. The regeneration frequency is more in MS media containing BAP (1.5 mg/L).Regenerated plantlets were further rooted on the medium in bottles. Thereafter, rooted plants were grown to maturity in the greenhouse and allowed to set seeds.

    Time:

    Title: Protein L-Isoaspartyl Methyltransferase (Pimt): an Enzyme to Increase Seed Vigor & Longevity

    Manoj Majee
    National institute Of Plant Genome Research, India.

    Biography
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    Biography

    Manoj Majee
    National institute Of Plant Genome Research, India.



    Abstract
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    Abstract

    Manoj Majee
    National institute Of Plant Genome Research, India.

    Seed longevity is a vital trait for the germplasam conservation of endangered and cultivated species. Maintenance of seed vigor and longevity during storage is also one of the important concerns in Agriculture, since under sub tropical climate, seeds of most crop species show rapid deterioration and exhibit reduced seed longevity. Recently in our lab, we demonstrated the role of Protein L-Isoaspartyl Methyl Transferase (PIMT) in enhancing seed vigor and longevity. PIMT is a protein repairing enzyme and catalyzes the conversion of spontaneously modified isoAsp to Asp in protein. Our study in rice (Oryza sativa) demonstrated that PIMT activity sharply increases at maturation phase, is retained in dry seed and then declines upon completion of germination. Likewise, deleterious isoaspartyl accumulation also increases during seed maturation and is highly abundant in dry seed but decreases upon imbibition. Rice possesses two genes (OsPIMT1 and OsPIMT2) which encode multiple PIMT isoforms. Transcript and western blot analyses clearly demonstrated distinct tissue and seed development stage specific accumulation of these PIMT isoforms, indicating their participation and specific contribution in seed desiccation, longevity and germination in rice. Further analyses of overexpression transgenic lines for each OsPIMT isoform revealed that they play a distinct role in restricting deleterious isoAsp and ROS accumulation to improve seed vigor and longevity. Overall, our study demonstrated that PIMT enzyme can be exploited to maintain high seed vigor ensuring a vigorous crop establishment in wide environmental conditions and would be substantial benefit to society

  • Keynote Speaker

    Time:

    Title

    Title: Tumor Extracellular Matrix Microenvironment, Role of Macrophages

    Henry Lopez
    Riptide Bioscience, USA.
    Biography
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    Biography

    Henry Lopez is a founder, CEO/CSO of MuriGenics, Inc. which is a preclinical contract research organization working with national and international companies, located in Vallejo, California. Prior to this he worked with many Northern California biotechnology companies such as Pfizer, Parke-Davis, GeneNetworks, Glycomed, Xoma and Cetus. In 2012 he founded Riptide Bioscience (Senior Vice President) a synthetic peptide company focusing on immunomodulation and antimicrobials. Have licensed out one peptide to Pharma for treating pancreatic cancer- working partner has been the NCI for the last 4 years. He received PhD from the University College London –Royal Free Hospital.



    Abstract
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    Abstract


    Keynote Speaker

    Time:

    Title

    Title: Transcriptome Profiling in Gastric Cancer

    Prakash Sharma
    Guru Gobind Singh Indraprastha University, India Biography
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    Biography

    Professor in Biotechnology, Ex-Dean University School of Biotechnology; Director (Research & Consultancy), and Director (Academic Affairs) at Guru Gobind Singh Indraprastha University, New Delhi, India. He has over 30 years teaching and research experience. His research interests have mainly focused on the development and use of molecular markers for diverse applications in crop plants, andin silico data mining to explore microsatellite dynamics in diverse genomes. Recently, NGS based transcriptomics has been exploited for identification of abiotic stress responsive elements in seabuckthorn, a plant with immense medicinal and nutraceutical value, and to understand molecular pathogenesis of hypobaric hypoxia and gastric cancer.



    Abstract
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    Abstract


    Sessions:
    Biotechnology and Applied Biochemistry

    Time:

    Title: Molecular Insights into Membrane Trafficking by the SNX27-retromer Complex

    Mintu Chandra
    The University of Queensland, Australia.

    Biography
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    Biography

    Mintu Chandra
    The University of Queensland, Australia.



    Abstract
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    Abstract

    Mintu Chandra
    The University of Queensland, Australia.

    Compartmentalisation is a defining feature of all eukaryotic cells, and we have evolvedhighly sophisticated protein machineries to control the flow of transmembrane moleculesand membrane lipids between different organelles. Disruption of these processes are linkedto numerous diseases including neurodegenerative disorders, pathogen invasion andcancer. We are determining how these trafficking machineries are assembled and regulatedat the molecular level through a combination of structural biology, biophysical, and cellbiology approaches. In my talk, I will describe our most recent work on critical protein sortingmachineries the retromer complex and the sorting nexins (SNXs) - regulating endosomalmembrane recycling and cellular homeostasis. We have defined sorting signals requiredfor endosomal recycling by the SNX27-retromer complex, how this is regulated by posttranslationalphosphorylation, and the structural basis for SNX27-retromer-cargoassembly.

    Time:

    Title: New Tool for Enzymatic Analysis in Raw Materials

    Julia Schuckel
    Glycospot, Denmark

    Biography
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    Biography

    Julia Schuckel
    Glycospot, Denmark

    Dr. Julia Schuckel holds a M.Sc. degree in chemistry from the Technical University of Dresden, Germany and a PhD degree in biochemistry from the University of York, United Kingdom. She developed together with her colleague Stjepan K. Kracun the screening technology for testing enzyme activities in raw material.



    Abstract
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    Abstract

    Julia Schuckel
    Glycospot, Denmark

    Industries, such as pharma, feed, and food, are increasingly looking for enzymes to solve production and product issues. Correct and adequate information on enzyme activity would help producers avoid prolonged production times, reduce waste, improve end-products, prolong shelf-life and save costs for overtreatment. Most of the production processes turning raw materials into products are under the influence of a wide variety of enzyme combinations. Existing tools for screening of enzymatic activity, are cumbersome, time-consuming and often requires expert staff. Each assay needs to be constructed from the bottom up forcing the user to aliquot substrates with high precision into a large number of reaction vessels manually. Furthermore, all existing assays measure the activity of one enzyme only at a time. We have developed a ready-to-use screening technology, where it is possible to detect important enzymatic activity in raw material. We will show different examples of endogenous enzymatic activity in the grain, flour and malt. This data helps to minimize uncertainties when using technical aids and eventually, desired properties of the final product are ensured.

    Time:

    Title: Determination of Haemolytic Effect and Spectral Analysis Using Gas Chromatography Mass Spectrometry (GC-MS), Fourier Transform Infrared (FTIR) and Ultraviolet Visible (UV-Vis) Spectroscopy of Different Extracts of Cucumis melo L. var. inodorus (Sweet Melon) Fruit

    Parker Elijah Joshua
    University of Nigeria, Nigeria

    Biography
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    Biography

    Parker Elijah Joshua
    University of Nigeria, Nigeria



    Abstract
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    Abstract

    Parker Elijah Joshua
    University of Nigeria, Nigeria

    The concept that fruits and vegetables contribute to a person’s wellbeing is as old as Hippocrates, the father of medicine, who more than 2000 years ago told his patients “let your food be your medicine and your medicine be your food”. Today, this same philosophy with regard to fruits and vegetables being more than just nutrition but medicine as well is experiencing rejuvenation. Indeed, a positive correlation has been reported between fruit consumption and the decreased risk of several chronic diseases including obesity, cardiovascular disease, and certain types of cancer etc. (Boeing et al., 2012; Jansen et al., 2011). The study is aimed at determining the haemolytic effect of Cucumis melo L.var. inodorus (sweet melon or honeydew melon) on human erythrocytes and to study this effect by different spectral analyses.

    Time:

    Title: The Mechanistic Role of Active Site Residues in Non-Stereospecific Haloacid Dehalogenase E (Dehe) using Computational Approach

    Azzmer Azzar Abdul Hamid
    International Islamic University Malaysia, Malaysia

    Biography
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    Biography

    Azzmer Azzar Abdul Hamid
    International Islamic University Malaysia, Malaysia



    Abstract
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    Abstract

    Azzmer Azzar Abdul Hamid
    International Islamic University Malaysia, Malaysia

    Dehalogenase E (DehE) is a non-stereospecific haloaciddehalogenase produced by soil bacteria, Rhizobium sp. RC1. This enzyme has been hypothesized to facilitate ‘direct attack mechanism’ whereby its activated water molecule will directly attack the α-carbon of haloacid compound, thus releasing the halogen. The similar mechanism was proved and identified for Dehalogenase I (DehI) from Pseudomonas putida PP3 which also classified under the same group with DehE. For a better understanding of the DehE catalytic mechanism, this enzyme was docked with D- and L- stereoisomer of 2-Chloropropionic acid (2CP) and the their complex structures were simulated using GROMACS 5.1.2 for 50 nano-seconds. Upon completion, the distance of water towards Asn114, Asp189 and the α-carbon of the 2CP were calculated. The same procedure was also applied to DehI as it serves as the benchmark. Analysis of DehE catalysis was revealed information on the presence of water molecule, water activation potential and direct attack mechanism. In this study, the catalytic water was found located nearby Asn114 and a hydrogen bond was formed between these molecules. Asn114 was seen to provide the water molecule that needed for the mechanism and play role as ‘water-bearer’. For water activation, the distance of Asp189 towards water molecule was found in the range of ~2 to ~8 Å which is favorable for the activation to occur. This aspartate residue was proposed to activate the catalytic water into hydroxide ion. While for the direct attack, the distance of water molecule towards the α-carbon of 2CP was approximately at 8 Å, which also the similar range that was observed in DehI-2CP. In conclusion, DehE is strongly suggested based on in silico analysis to facilitate ‘direct attack mechanism’ for haloacid catalysis. This study information will provide a platform for isotope labeling experiment and protein engineering for non-stereo haloaciddehalogenase of Rhizobium sp. RC1.

    Sessions:
    Biotechnology for Genetic disorders

    Time:

    Title: Genomic Modification By Ocimum Canum Against Lead-Induced Chromosom -Aberration And Its Effect On Antioxidant Enzymes

    Oladimeji Tugbobo
    Federal Polytechnic, Nigeria.

    Biography
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    Biography

    Oladimeji Tugbobo
    Federal Polytechnic, Nigeria.



    Abstract
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    Abstract

    Oladimeji Tugbobo
    Federal Polytechnic, Nigeria.

    Anticlastogenic potential of Ocimum canum (Black leaf) extract was studied in bone marrow cells of mice using micronucleus assay. 200mg/kg of Ocimum canum aqueous extract was administered as dietary supplement for 30-days. The mice were divided into three groups A, B and C. Animals in group A were fed with distilled water, B were treated with 2.5mg/kg lead acetate while group C were fed with 200mg/kg Ocimum canum aqueous extract and 2.5mg/kg lead acetate simultaneously. After 30-days, mice were sacrificed and chromosome preparations were made from bone marrow according to colchicines hypotonic-fixation air drying Giemsa schedule. The cytogenic end-point observed was chromosomal aberration which increased significantly (P<0.05) in group B animals treated with lead acetate only. However, the chromosomal aberration was significantly (P<0.05) reduced by the extract fed to animals in group C. In addition, the effect of the extract on the defensive antioxidant enzymes of the test animals was also assessed. The results indicate synergistic effect of the extract on the antioxidant enzymes in the liver tissues. Hence, the results of this study suggest viable anticlastogenic and antioxidant potentials of Ocimum canum extract which could protect against lead-induced chromosomal aberration and as well enhance activities of antioxidant enzymes.

    Sessions:
    Biotechnology Applications in Genomics and Proteomics

    Time:

    Title: Sensitivity of ITT1Δ and RPS1AΔ Yeast Strains to Arsenite Reveals their Role in Translation of URE2, a Key Gene Involved in Metal Detoxification

    Houman Moteshareie
    Carleton University, USA

    Biography
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    Biography

    Houman Moteshareie
    Carleton University, USA

    Houman completed hisBachelor's degree in Cambridge, UK in Medical Genetics. He continued his education with obtaining a Master’s degree from University of Wales, UK in Biotechnology and Molecular Genetics. He is now in the final year of his PhD at Carleton University, Canada in Molecular Genetics and Systems Biology. Houman is currently a lecturerat Carleton University and Algonquin College.His research focuses on identification and investigation ofnovel genes that are involved in protein biosynthesis. In particular, he is working on Internal Ribosome Entry Sites (IRES) utilizing baker's yeast,Saccharomyces cerevisiae as a model organism. During his graduate education, Houman has been involved in many research and collaborated with excellent scientists, which resulted in many scientific publications.



    Abstract
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    Abstract

    Houman Moteshareie
    Carleton University, USA

    Heavy metal and metalloid contaminations are among the most concerning pollutions in the world. Many parts of the world are polluted with toxic heavy metals and metalloids. Consequently, investigating the molecular mechanisms that drive the detoxification pathways in living organisms is of importance. A variety of genes have known functions in detoxification. The expression of these genes is controlled at both transcriptional and translational levels. When under heavy-metal stress, 5’ cap-dependent translation is often down-regulated or even completely inhibited. In baker’s yeast, Saccharomyces cerevisiae, resistance to a wide range of toxic metals is regulated by glutathione S-transferase products. Yeast URE2 protein has glutathione peroxidase activity and is homologous to mammalian glutathione S-transferases. In addition to 5’ cap-dependent translation, yeast URE2 mRNA is also translated through an internal structure, free of 5’ cap and eIF4E. It possesses a unique Internal Ribosome Entry Site (IRES) element between nucleotides 205 and 309 in its coding region. URE2 deletion mutants are hypersensitive to toxic metals such as arsenic, nickel and cadmium. Here, we report on the finding of two genes, ITT1, inhibitor of translation termination, and RPS1A, ribosomal protein 10 whose deletion strains exhibit similar drug sensitivity phenotype as URE2 mutant. Neither of these genes were previously linked to metal toxicity. Our gene expression analysis illustrates that these two genes affect URE2 mRNA expression at the translational level. Subsequent investigations suggest that the deletion of ITT1 or RPS1A may negatively regulate the translation of URE2 mRNA through its IRES element. Polysome profile analysis suggest a strong tie between candidate genes and protein synthesis mainly at the initiation step. Our genetic interaction analysis also showed strong communication between our two candidate genes and the genes involved in the regulation of translational and translation initiation.

    Time:

    Title: CRISPR/CAS9 Targating Micro RNA-24 in Chinese Hamster Ovary Cells Increases Growth and Boosts Productivity

    Kevin Kellner
    NICB, Ireland.

    Biography
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    Biography

    Kevin Kellner
    NICB, Ireland.



    Abstract
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    Abstract

    Kevin Kellner
    NICB, Ireland.

    Chinese Hamster Ovary (CHO) cells are the prominent cell line used in biopharmaceutical production. Although optimisation efforts have led to a vast increase in productivity, CHO cells yield less than other expression systems like yeast or bacteria. To improve yields and find beneficial bioprocess phenotypes, genetic engineering plays an essential role in recent research. The miR-23 cluster with its genomic paralogues (miR-23a and miR-23b) was first identified as differentially expressed during temperature shift, suggesting its role in proliferation and productivity. The common approach to deplete miRNAs is the use of a sponge decoy which, requires the introduction of reporter genes. As an alternative this work aims to knockdown miRNA expression using the recently developed CRISPR/Cas9 system which does not require a reporter transcript. This system consists of two main components: the single guide RNA (sgRNA) and an endonuclease (Cas9) which induces double strand breaks (DSBs). These DSBs can result in insertion or deletion (indels) of base pairs which can disrupt miRNA function and processing. A CHO-K1 cell line stably expressing an IgG was used for knockdown experiments. SgRNAs were designed to target the seed region of miR-24-3p and stable mixed populations were generated. It was shownthat miRNA expression for miR-24-3p as well as miR-24-5p was significantly reduced in mixed populations. Furthermore, an increase in pre-miR-24 was exhibited suggesting impaired processing by Drosha or Dicer. A knockdown up to 95% was achieved for miR-24 as well as the passenger miRNA (Figure 1A, B and C). The other members of the cluster which are located proximal to miR-24 were not affected by the knockdown (Figure 1D). Depletion of miR-24 showed increased proliferation in batch culture as well as boosted productivity (Figure 1E and F). However, faster growth led to increased nutrient demand of miR-24 depleted cultures and a reduction in culture time was exhibited. Quantitative label-free LC/MS was used to identify 81 more abundant protein targets. Pathway analysis revealed proteins potentially involved in enhanced ribosomal RNA biogenesis, recycling and assembly of ribosomal subunits. Furthermore, proteins involved in catalysing the loading of cognate aminoacyl-tRNAs and release of deacetylated tRNAs were higher expressed. These targets were highlighted as potential cell line engineering targets to improve productivity of CHO cells. Out of all 81 upregulated proteins, 50 were predicted targets of either miR-24-3p or miR-24-5p. In this work, we have shown that CRISPR/Cas9 can be successfully applied as a tool to knockdown miRNA expression in CHO cells. The data was generated using mixed pools and it remains to be established if both alleles can be successfully targeted e.g. using next-generation sequencing ofindividual clones.

    Sessions:
    Cell and Molecular Biology

    Time:

    Title: Manipulation of tRNA Genes to Enhance Cellular Productivity of Biologics

    Bob White
    York University, England.

    Biography
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    Biography

    Bob White
    York University, England.

    Bob White studied BiochemistryatUniversity of Oxford and Molecular Biology at the National Institute for Medical Research. He then spent 5 years at University of Cambridge before establishing his own laboratory at University of Glasgow. In 2013 he became Chair of Biochemistryat University of York. His research focuses on gene expression and has been published in Nature, Science and Cell (>6400 citations, h-index 44). He has received several national and international awards and is a Fellow of The Royal Society of Edinburgh, The Academy of Medical Sciences and The European Academy of Cancer Sciences.



    Abstract
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    Abstract

    Bob White
    York University, England.

    Recombinant monoclonal antibodies are a powerful class of therapeutics that are used to treat a wide range of diseases, including cancers and inflammatory disorders. However, they are expensive to produce and this restricts their availability to patients; health authorities sometimes cannot afford the costs of these therapies. Lowering production costs may increase access to potentially life-saving treatments. Through synthetic cell bioengineering, we are developing novel approaches to improve production of therapeutic proteins. One of our strategies is to manipulate tRNA expression to enhance translation of recombinant products. Our progress in this regard will be described. We aim to reduce costs and thereby increase availability to patients of therapeutics that at present are prohibitively expensive.

    Time:

    Title: Regulation of Virulence in P. Aeruginosa by Mechanical Cues

    Albert Siryaporn
    University of California, USA.

    Biography
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    Biography

    Albert Siryaporn
    University of California, USA.

    Dr. Siryaporn received his Ph.D. in Physics and Astronomy at the University of Pennsylvania with Prof. Mark Goulian in 2008 studying cross-talk between signaling networks in bacteria. As a postdoc in the Department of Molecular Biology at Princeton University, he investigated physical mechanisms that regulate the ability of bacteria to infect. He received the NIH Kirschstein NRSA postdoctoral fellowship and the NIH K Career Transition Award for his work. Dr. Siryaporn is currently an assistant professor in the Physics and Astronomy and Molecular Biology and Biochemistry Departments at the University of California, where his lab studies bacterial pathogenesis at the intersection of biophysics and molecular biology.



    Abstract
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    Abstract

    Albert Siryaporn
    University of California, USA.

    We explore how bacteria detect and respond to mechanical forces. During the course of an infection, bacteria encounter a variety of mechanical forces such as adhesive forces during contact with the host cells that they infect and shear stresses in fluidic environments. We have developed a biophysical approach using microfluidics and fluorescence lifetime imaging microscopy to explore how bacteria interpret mechanical cues to detect the presence of host cells and to guide the expansion of large bacterial populations within host organisms. In particular, we found that the pathogen Pseudomonas aeruginosa detects the presence of hosts using a mechano-sensitive mechanism, akin to a bacterial sense of touch. This response activates virulence and consequently P. aeruginosa, unlike other pathogens, relies on mechanical input rather than exclusively on chemical signals for infection. This model provides a long-sought explanation for understanding how P. aeruginosa can infect a broad range of hosts including humans, animals and plants. The ubiquity and diversity of mechanical forces in all aspects of a bacteriums life have far-reaching consequences that we are just beginning to comprehend.

    Sessions:
    Environmental Biotechnology and Biodiversity, Waste water treatment

    Time:

    Title: Removal of Lead and Mercury from Contaminated Water

    Hossein Rostami
    Philadelphia University, USA

    Biography
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    Biography

    Hossein Rostami
    Philadelphia University, USA



    Abstract
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    Abstract

    Hossein Rostami
    Philadelphia University, USA

    Over the past 15 years, about one half million sites with potential contamination have been reported to federal or state authorities. Of these, about 217,000 sites still need remediation and new contaminated sites continue to appear each year. The most common type of contaminants is metals, solvents and petroleum products. Heavy metals are present in two thirds of Department of Defense (DOD) and superfund sites and about 50% of Department of Energy (DOE) and Resource Conservation and Recovery Act (RCRA) sites. Heavy metals are the largest class of contaminates and also the most difficult to treat. Heavy metals most frequently found in the waste stream are lead, mercury, chromium, cadmium, arsenic and zinc. This work focuses on the removal of lead from aqueous solution. Lead and Mercury has long been acknowledged as a harmful environmental contaminant. Lead pollution impacts all of the body's systems. Lead exposure can adversely influence the brain, central nervous system, blood cells, and kidneys. Mercury exposure can adversely influence entire body including the brain, central nervous system, lung, skin, and kidneys. To remove lead and mercury from wastewater, several methods are utilized. Some of these methods are chemical precipitation, ion exchange adsorption, reverse osmosis and electrodialysis. All of these techniques have shortcomings, Alkali Fly Ash Permeable Reactive Barrier (AFA-PRB) is a newly developed novel material made with fly ash alkali activating solution and filler material (sand and coarse aggregates). AFA-PRB was used to remove leadand mercury from contamination water. AFA-PRB materials with high permeability were created. For reactive barrier material permeability, must be rapid, in the range of 10-2 cm/sec to 10-1 cm/sec. AFA - PRB from three ash sources with permeability of 10-1 cm/sec were produced and crushed into pelletized form. Effectiveness of the various barriers was determined by batch and column tests. Laboratory experiment indicates lead ion reduces from 1000 ppm to less than 2 ppm with 10 liters of solution and Lead ion from 10 ppm to less than 0.01 ppm. . Laboratory experiment also indicates mercury ion reduces from 1000 ppm to less than 0.5 ppm with 10 liters of solution and mercury ion from 10 ppm to less than 0.01 ppm.

    Time:

    Title: Removal of Lead and Mercury from Contaminated Water

    Mozhgan Bahadory
    Philadelphia University, USA

    Biography
    χ

    Biography

    Mozhgan Bahadory
    Philadelphia University, USA



    Abstract
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    Abstract

    Mozhgan Bahadory
    Philadelphia University, USA

    Over the past 15 years, about one half million sites with potential contamination have been reported to federal or state authorities. Of these, about 217,000 sites still need remediation and new contaminated sites continue to appear each year. The most common type of contaminants is metals, solvents and petroleum products. Heavy metals are present in two thirds of Department of Defense (DOD) and superfund sites and about 50% of Department of Energy (DOE) and Resource Conservation and Recovery Act (RCRA) sites. Heavy metals are the largest class of contaminates and also the most difficult to treat. Heavy metals most frequently found in the waste stream are lead, mercury, chromium, cadmium, arsenic and zinc. This work focuses on the removal of lead from aqueous solution. Lead and Mercury has long been acknowledged as a harmful environmental contaminant. Lead pollution impacts all of the body's systems. Lead exposure can adversely influence the brain, central nervous system, blood cells, and kidneys. Mercury exposure can adversely influence entire body including the brain, central nervous system, lung, skin, and kidneys. To remove lead and mercury from wastewater, several methods are utilized. Some of these methods are chemical precipitation, ion exchange adsorption, reverse osmosis and electrodialysis. All of these techniques have shortcomings, Alkali Fly Ash Permeable Reactive Barrier (AFA-PRB) is a newly developed novel material made with fly ash alkali activating solution and filler material (sand and coarse aggregates). AFA-PRB was used to remove leadand mercury from contamination water. AFA-PRB materials with high permeability were created. For reactive barrier material permeability, must be rapid, in the range of 10-2 cm/sec to 10-1 cm/sec. AFA - PRB from three ash sources with permeability of 10-1 cm/sec were produced and crushed into pelletized form. Effectiveness of the various barriers was determined by batch and column tests. Laboratory experiment indicates lead ion reduces from 1000 ppm to less than 2 ppm with 10 liters of solution and Lead ion from 10 ppm to less than 0.01 ppm. . Laboratory experiment also indicates mercury ion reduces from 1000 ppm to less than 0.5 ppm with 10 liters of solution and mercury ion from 10 ppm to less than 0.01 ppm.

    Time:

    Title: Diversity of Microorganisms in Heavy Oil Reservoirs (Russia) and their Possible Application in Meor

    Diyana Sokolova
    Russian Academy of Sciences, Russia

    Biography
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    Biography

    Diyana Sokolova
    Russian Academy of Sciences, Russia

    Dr. Diyana Sokolova, graduated from the Mendeleyev University of Chemical Technology of Russia (MUCTR) in 2001. From 2001 she has been working as a researcher at the Laboratory of Petroleum Microbiology, Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences,Moscow, Russia. She did her Ph.D., on "Formation of biosurfactants by aerobic organotrophic bacteria from oil reservoir", under the supervisor Dr. T.N. Nazina.DiyanaSokolova worked at petroleum reservoirs of Russia, China and USA. Areas of specialization: petroleum microbiology, biosurfactants, physiology of microorganisms, heavy oil,biodegradation, biotechnology, microbial enhancement for oil recovery (MEOR).



    Abstract
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    Abstract

    Diyana Sokolova
    Russian Academy of Sciences, Russia

    Geological reserves of heavy and high-viscosity oil in Russia reach 6–7 billion tons and exceed residual reserves of light conventional oil. The goal of the present work was to study the diversity and activity of microorganisms from low-temperature heavy oil reservoirs (Russia) and their biotechnological potential for development of the microbiological method for enhanced recovery of heavy oil. Microorganisms are able to produce diverse oil-displacing agents (organic and mineral acids, biosurfactants, solvents, biopolymers, gases), which can be in technologies for microbial enhancement of oil recovery (MEOR). The functional and phylogenetic diversity of microorganisms at five heavy oil deposits (Russia) was studied. The oilfieldsexploited without water-flooding, were shown to harbor scant microbial communities, while microbial numbers in the water-flooded strata was high. Methanogens of the genera Methanothrix,Methanobacterium, and Methanoregulawere revealed by high-throughput sequencing of 16S rRNA genes and DGGE analysis of mcrAgenes. Aerobic bacteria of the genera Rhodococcus, Pseudomonas, Gordonia, Cellulomonas, etc., capable of biosurfactant production, were isolated. Fermentative enrichments producing volatile organic acids (acetic, propionic, and butyric) from sugar-containing substrates were obtained. These acids dissolved the carbonates of oil-bearing rock efficiently. The major microbial groups of interest for development of MEOR technology are aerobic oil-oxidizing bacteria, anaerobic fermenting bacteria, and methanogenic prokaryotes. Core-flooding experiments with injection culture liquid of aerobic bacteria Gordoniaamicalis6-1 and Rhodococcuserythropolis KS22 resulted in recovery of 17.6% of additional oil from the models because of biosurfactants and biomass production. Application of starch and culture liquid of fermenting enrichment capable of producing low fatty acids resulted in recovery of 13.4% of additional oil from the models.Selection of the most efficient microbial technology for enhanced recovery of heavy oil from terrigenous and carbonate strata is discussed.

    Time:

    Title: Assessing the Microbiome Dynamics in Three Photo-Bioreactors Established for Coking Wastewater Treatment: An Orchestration between Microalgae and Bacterial Communities

    Mariam Hassan
    Cairo University, Ezypt

    Biography
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    Biography

    Mariam Hassan
    Cairo University, Ezypt

    Mariam Hassan is an Assistant Lecturer in Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University. She teach the practical courses to the undergraduate and graduate students. She is working on my PhD project on biomonitoring water microbiome(s) involved in biological wastewater treatment processes. She had a experience on experience: microbiology, biotechnology, biodiversity, bioinformatics and high through put data analysis.



    Abstract
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    Abstract

    Mariam Hassan
    Cairo University, Ezypt

    The investigation of microbial community structures is a significant way to understand biodegradation capacities in biological wastewater treatment processes. Photo-bioreactors A, B and C received real coking-wastewater as influent with COD 776 ± 56, 1229 ± 85 and 2033±27 mg/l, respectively. In phase-1 phenol was added to the influent, while dichlorophenol was added in combination with phenol in phase-2. Treatment efficiency of algal-bacterial systems was biomonitored using different bioassays (phytotoxicity, Artemia toxicity, cytotoxicity, algal-bacterial ratio and settleability). COD removal %, phenol and dichlorophenol concentrations were also monitored. All systems efficiently detoxified the influents in phase-1. In phase-2, Systems B and C failed to detoxify the influents. Illumina-sequencing generated 2119749 effective sequences of 16S-rRNA gene from 21 samples collected from different influents and effluents. The number of observed species was significantly lower in effluent samples than influent samples, as some taxa dominated in photo-bioreactors and contributed to the systems performance. Significant difference in the microbial diversity between influent and effluent samples was detected. Proteobacteria (78 %), Firmicutes (12 %), Bacteroidetes (5 %) and Deferribacteres (2 %) were the dominant phyla in influent samples. While in effluent samples Proteobacteria (68 %) and Bacteroidetes (25 %) dominated. Failure in treatment process in systems B and C at phase-2 was accompanied with significant difference in the microbial diversity. Significant relative abundance of anaerobic bacteria from Deferribacteraceae and Peptococcaceae families in influent samples conformed to the nature of coking-wastewater. The co-culture of microalgae shifted the microbiome and promoted the activity of genera affiliated to Chitinophagaceae, Pseudomonadaceae and Xanthomonadaceae families, which dominated in effluent samples. These bacteria are known for their catabolic diversity that enables xenobiotic degradation. The superiority of algal-bacterial systems for coking-wastewater treatment was confirmed as co-culture of microalgae eradicated pathogenic bacteria such as Arcobacter and Legionella genera in the treated effluent.

    Time:

    Title: Phenotypic and Molecular Characterization and Rapid Evaluation of Oil-degrading Native Bacteria Isolated from Different Habitats in UAE

    Ismail Saadoun
    Sharjah University, UAE

    Biography
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    Biography

    Ismail Saadoun
    Sharjah University, UAE



    Abstract
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    Abstract

    Ismail Saadoun
    Sharjah University, UAE

    Despite the beneficial value of crude oil and its derivatives to the country’s economy, it could have a huge adverse impact on the environment specifically when accidently spilled to the water and soil; thus, makes it as an environmental catastrophe. This study addresses the role of identified microorganisms as hydrocarbon degraders to be used as an eco-friendly solution in oil spill bioremediation. Successfully, 19 bacterial isolates were recovered from different habitats including crude oil wells, soil treated with diesel, oil contaminated seawater and surface hydrocarbon sediment. The isolates were assessed for degradation of different hydrocarbon compounds by agar hole-plate diffusion method. Results indicated the recovery of 9 isolates namely (2A, 1D, So1, S1A, S3, KF1, SO2, AJ1 and 2B) which were identified as positive degraders for one or more of the tested hydrocarbon compounds including diesel, pentane, hexane, heptane and tetradecane. These isolates were identified biochemically using VITEK 2 microbiology system as 2A (Pseudomonas stutzeri 97%),1D (Kocuriakristinae 92%), SO1 (Staphylococcus aureus 93%), S1A (Leuconostocmesenteroides ssp. Cremoris 90%), KF1 (Rhizobium radiobacter 99%), and S3 (Staphylococcus hominis96%). Almost all of these isolates were able to utilize heptane as a sole carbon source for their survival with the isolates SO2, S1A and S3 being the most potent ones observed by their growth around the agar hole-plate. PCR analysis of the positive hydrocarbon degrading isolates for the presence of alkBgene showedtwo groups with different band size products; group 1 (G1) (~330 bp) and group 2 (G2) (multiple of 330 pb). This may imply that alkB gene can be found in multiple homologues as shown in G2 and each one may cover degradation of specific carbon number range in the tested hydrocarbon compound. Rapid evaluation of hydrocarbon compounds degradation by the native microbial communities was shown to be successful with considerable biodegradation role exploited by the recovered isolates.

    Time:

    Title: Microbial Decolorization of Reactive Azo Dyes by Bacillus Spp. Under Anaerobic Condition

    Maulin P Shah
    Enviro Technology Limited, India.

    Biography
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    Biography

    Maulin P Shah
    Enviro Technology Limited, India.

    His laboratory is trying to assess the impact of industrial pollution on microbial diversity of waste water following cultivation dependant and cultivation independent analysis. His major work involves isolation, screening, identification and Genetic Engineering of high impact of Microbes for the degradation of hazardous materials. Dr. Maulin P Shah has more than 150 research publication in highly reputed national & international journals. He is Editor-in-Chief of Research Journal of Microbiology, Journal of Biotechnology & Bio materials.



    Abstract
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    Abstract

    Maulin P Shah
    Enviro Technology Limited, India.

    A bacterial strain Bacillus spp. with remarkable ability to decolourize the reactive azo dyes such as reactive red, reactive yellow, reactive brilliant red and reactive brilliant blue was isolated from the textile effluent contaminated site. The effluent sample was collected from local text textile industry, Ankleshwar, Gujarat, India Static conditions with 10 g/l glucose, pH 9, 37°C, 20% inoculum concentration, 50 mg/l of dye concentration, 3 g/l of NH4NO3, were considered to be the optimum decolourizing conditions. Bacillus spp. grew well in these optimum conditions, resulting in 82% decolourization extent 7 days of incubation. Phenotypic characterization and phylogenetic analysis of the 16S rRNA sequence indicated that the bacterial strain belonged to the genus Bacillus. UV (Ultra Violet) analysis of bacterial isolate suggested that it exhibited decolourizing activity, rather than inactive surface adsorption. This decolourization extent and facile conditions show the potential for this bacterial strain to be used in the biological treatment of textile effluent or dyes.

    Time:

    Title: Valorization of Wastes of Organic Food Industries for the Amendment of Agricultural Crops

    Dounia Lakhal
    University of Hassan II Casablanca, Morocco

    Biography
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    Biography

    Dounia Lakhal
    University of Hassan II Casablanca, Morocco



    Abstract
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    Abstract

    Dounia Lakhal
    University of Hassan II Casablanca, Morocco

    Waste of agri-food industries is very varied and varies according to the seasons. They are mainly composed of organic waste generated by the processing activities of plant and animal products. This waste has a negative impact on the environment; nevertheless, his transformation, especially through the biological process of biotransformation, can generate a new economic source by creating a stable and balanced product that can be used as fertilizer because of its content of basic elements: nitrogen, potassium and phosphorus. This work presents the results of a valorization test of certain waste of agro-food industry, three different wastes were combined: Fish waste, molasses and peels of potato. Several balanced mixtures were developed and underwent natural biotransformation for 2 weeks to produce a fertilizer of good nutritional and microbiological quality. Quality control parameters (pH, temperature, conductivity, dry matter, phosphorus, nitrogen, total carbon ...) were monitored during the time stipulated for biotransformation. The results show that the fertilizer generated from the mentioned waste is mature from the 8th day, it has a good hygienic quality, due to the absence of the spoilage microorganisms, rich in nitrogen, phosphorus, potassium, carbon and characterized by a stable optimal pH. Application tests on a barley crop confirmed its effect. Mathematical modeling, in the form of a ternary diagram, of the quality of the final product according to the composition of the initial mixture is established to build in practice a predictive operational model for industrial waste management.

    Sessions:
    Food Biotechnology

    Time:

    Title: Geographical Origin of Food by Multivariate ToF-SIMS Analysis

    Marco Consumi
    University of Siena, Italy

    Biography
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    Biography

    Marco Consumi
    University of Siena, Italy

    Dr. Marco Consumi is a post-doc at university of Siena. He received his Ph.D. in Biomaterials on hydrogel biomedical application and, actually his research focus on polymer based materials for controlled release of active substances in pharmaceutical and nutraceutical field. As a postdoctoral fellow, he was focused on understanding the correlation between the chemical composition of materials and their biological activity. He has also broad expertise in synthesis modification and characterization of polymers (naturals and synthetics) and materials for biomedical applications. Actually, involved in 2 EU projects on bacterial biofilm.



    Abstract
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    Abstract

    Marco Consumi
    University of Siena, Italy

    In this work, time-of-flight secondary ion mass spectrometry (ToF-SIMS) in conjunction with Multivariate analysis (MVA) are applied to study the chemical composition and variability of different food matrices: - Sardinian myrtle (Myrtus communis L.): analysis of both berries alcoholic extracts and berries epicarp. through the. ToF-SIMS spectra of berries epicarp show that the epicuticular waxes consist mainly of carboxylic acids with chain length ranging from C20 to C30, or identical species formed from fragmentation of long-chain esters. PCA of ToF-SIMS data from myrtle berries epicarp distinguishes two groups. Seggianese olives and olive oil: three different groups of Seggianese olives: (i) treated with an insecticide (dimethoate) and a fungicide (copper oxychloride) (TU); (ii) untreated (UT); and (iii) treated-washed (TW) have been analyzed by TOF-SIMS. Intact olive slices and olive oil were analyzed. Principal component analysis (PCA) of the Tof-SIMS spectra was used to investigate similarities among samples. Peaches and nectarine: samples of yellow-fleshedpeaches (Prunus persica L. Batsch) and yellow flesh nectarines (Prunus persica L. Batsch, var. Nectarina) of four different cultivars from areas of Southern Italy have been analyzed. ToF-SIMS analysis confirmed the presence of Cyanidin and Phosphatidylcholine in the skin of peaches and nectarines, and Cyanidin, Phosphatidylcholine, Oleic Acid and Coniferyl Alcohol in the skin of seed. ToF-SIMS with statistical data analysis is a promising method for thoroughly investigating the chemical composition and variability of food and natural products, allowing to extrapolate information on geographical origin and possible adulteration

    Time:

    Title: Exploration of Microbial Diversity of Traditional Fermented Foods and Beverages of North Western Himalayas for Probiotic Potential

    Savitri
    Himachal Pradesh University, India.

    Biography
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    Biography

    Savitri
    Himachal Pradesh University, India.

    Dr. Savitri has done her M.Sc. (Biotechnology) and Ph.D. from Himachal Pradesh University, Shimla in the year 2001 and 2007, respectively and also done her Post Doctoral Research from the same institute.Her area of specialization is food fermentation and probiotics. She has 9 years of teaching and 14 years of research experience. Presently, she is working as Assistant Professor in the Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla, India. She has published 24 research papers in reputed journals and 9 chapters in various books. She is a Life Member of Association of Microbiologist of India, Association of Biotechnologists of Himachal Pradesh and Indian Science Congress Association and Founder Member of Swedish South Asian Network of Fermented Foods, Sweden.



    Abstract
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    Abstract

    Savitri
    Himachal Pradesh University, India.

    Himachal Pradesh is small Indian state situated in north-western part of Himalayas.A range of traditional fermented products are prepared and consumed in Himachal Pradesh and the types of traditional fermented products of Himachal are unique and different from other areas. Bhatooru, chilra, seera, siddu, gulgule, marchu, sepubariand pickle from various locally available fruits and vegetablesviz.lingdi, aaro, elon, dheu, beedana, lasuda, chukhand different beverages like angoori, kinnauri, chhang, sura, behmi, etc. are some indigenous fermented products of Himachal Pradesh. These foods and beverages form a very important part of the sociocultural life of people in these areas. Microbiological studies of these fermented foods revealed that yeast Saccharomyces cerevisiae and bacteria belonging to Bacillus, Leuconostoc and lactic acid bacteria were mainly associated with these fermentations. 130 bacterial isolates were isolated from these foods and beverages. After preliminary screening, 20 isolates were assessed forprobiotic attributes. Among these, lactic acid bacterial isolates namely, Lactococcuslactis, Leuconostocmesenteroides, Pediococcuspentosaceus, Lactobacillus plantarum and Enterococcus faecalisshowed good probiotic properties. These organisms also showed antimicrobial activity against Escherichia coli, Bacillus cereus, Staphylococcus aureus and Shigelladysenteriae.These isolates can be further used as potential probiotic candidates for development of probiotic products.

    Time:

    Title: Fermentative Production and Statistical Optimization of Xylitol Using Novel Isolates of Candida parapsilosis Strain BKR1 in Indigenously Designed Multiphase Reactor

    Balakrishnaraja R
    Bannari Amman Institute of Technology, India

    Biography
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    Biography

    Balakrishnaraja R
    Bannari Amman Institute of Technology, India



    Abstract
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    Abstract

    Balakrishnaraja R
    Bannari Amman Institute of Technology, India

    Xylitol is a natural polyol and most widely known for its sugar substitute properties in diabetic patients. It is also used against the oral bacterial population. Most fascinating approach for commercial production of xylitol involves the suitable yeast fermentation. In this present investigation, factorials Optimization of these medium and process conditions are studied. Xylitol production by Candida parapsilosis strain BKR1 using Plackett-Burman and RSM are reported in modified minimal medium. The Plackett-Burman screening design reports the significant medium components are Xylose, yeast extract, Potassium Dihydrogen phosphate and magnesium sulphate. Further factorial optimization using face centred central composite design reveals the optimum levels of the significant medium components as Xylose – 104.69 g/l, Yeast Extract – 4.12 g/l; KH2PO4 – 2.84 g/l and MgSO4⋅7H2O – 2.09 g/l. Also the process parameters such as agitation, pH, Temperature and Inoculum level were optimized and validated as Agitation: 107 rpm, pH- 5 Temperature – 29.9ºC, Inoculum level – 1 ml. Bioreactor was designed and a pilot scale study was being carried out.

    Sessions:
    Nano Biotechnology

    Time:

    Title: Nanomaterials for Enzymatic Immobilization

    Rebecca Pogni
    University of Siena, Italy.

    Biography
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    Biography

    Rebecca Pogni
    University of Siena, Italy.

    Rebecca Pogni is Associate Professor of Physical Chemistry. Participant in European and national projects and coordinator of the EU project BISCOL "Bioprocessing for sustainable production of coloured textiles". Author of more than 80 papers on international journals on structure-activity relationship studies on enzymatic systems and protein radicals, enzymatic immobilization and synthesis of novel compounds and LCA studies. Representative for the University of Siena in the Italian cluster SPRING (Sustainable Processes and Resources for Innovation and National Growth), member of the National Consortium on the Development of Systems with Great Interface (CSGI) and member of the Life Cycle Assessment (LCA) network.



    Abstract
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    Abstract

    Rebecca Pogni
    University of Siena, Italy.

    In recent years nanoparticles and nanoscale materials have generated a great deal of interest from scientists and engineers of nearly all disciplines. This interest has been motivated to a large extent by reports that a number of physical properties including optical and magnetic properties, specific heats, melting points, and surface reactivity are size-dependent. These size-dependent properties are widely believed to be a result of the high ratio of surface to bulk atoms as well as the bridging state they represent between atomic and bulk materials. The interest in biocatalysts for biotechnological applications continues to grow. The fragile nature, high cost, and high loadings required for commercial production limits the use of free enzymes. Enzyme immobilization is utilized to surmount the stability, recovery, and recyclability disadvantages of using enzymes in solution, making them industrially and commercially viable Nanomaterials are particularly suitable for enzymatic immobilization. Among nanomaterials, nanofibers represent one of the most attractive nano-device for the production of high added value products. When the diameters of polymer fiber materials are shrunk from micrometers to sub-microns or nanometers there appear several amazing characteristics such as very large surface area to volume ratio flexibility in surface functionalities, and superior mechanical performance (stiffness and tensile strength) compared with any other known form of the material. Membranes of electrospun nylon and polyurethane nanofibers mesoporous silicas or magnetic nanoparticles can be synthesized, functionalized and used for enzymatic immobilization.

    Time:

    Title: Preparation and Characterization of Oral Drug Delivery System Based on Β-Lactoglobulin Nanoparticles Encapsulating Mangiferin

    Rohini Samadarsi
    National Institute of Technology, India

    Biography
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    Biography

    Rohini Samadarsi
    National Institute of Technology, India



    Abstract
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    Abstract

    Rohini Samadarsi
    National Institute of Technology, India

    Mangiferin, a bioactive Xanthonoid known for its anti-oxidizing & therapeutic nature, has been involved in a variety of potential pharmacological activities. The aim of this work is to encapsulate Mangiferin in betalactoglobulin nanoparticles by desolvation method. The prepared nanoparticles has an encapsulation efficiency of 87.56 ± 0.47%, particle size of average diameter 89.6 ± 10.4 nm and zeta potential of -33mv ± 0.2 mV. In vitro release kinetics of mangiferin from nanoparticles by simulated gastrointestinal studies showed that the mangiferin nanoparticles was relatively resistant to pepsin digestion. Maximum release of 82 ± 2.1% of mangiferin from nanoparticles took place in colon fluid. The slow release of mangiferin from the nanoparticles showed increased bioavailability. The study has important implication in the formation and design of encapsulated bioactive systems.

    Sessions:
    Immunology and Therapeutic Antibodies

    Time:

    Title: Fine Tuning of the Silver Bullet; Epigenetic Manipulation of Immune Checkpoints in Breast Cancer

    Hend M. El Tayebi
    German University in Cairo, Egypt.

    Biography
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    Biography

    Hend M. El Tayebi
    German University in Cairo, Egypt.

    Dr. EL Tayebi is an assistant professor of Genetics and Genetic Engineering and she is heading the “Genetic Pharmacology Research Group” in the faculty of Pharmacy and Biotechnology, German University in Cairo (GUC), Egypt. El Tayebi received her bachelor degree with highly honored excellence from faculty of Pharmacy and Biotechnology, GUC, in 2008. In 2009, she has been appointed as assistant lecturer of molecular pathology and pathophysiology at the GUC. And in 2014, she started teaching Cell Biology and Genetic Engineering as an assistant professor. She pursued her M.Sc. and PhD studies in the field of Molecular Pathology in the period from 2008-2012. She received her PhD degree with “Summa cum laude” from the GUC in 2012. After PhD, El Tayebi worked as a senior scientist and as a team leader of hepatocellular carcinoma (HCC) group under the umbrella of the MPRG. During her postdoctoral period (2012-2015), she focused on developing the research skills of her HCC team as well as herself by developing new lab techniques, writing proposals and earning research funds.



    Abstract
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    Abstract

    Hend M. El Tayebi
    German University in Cairo, Egypt.

    Programmed cell death protein-1 (PD-1) is an immune checkpoint receptor that prevents overstimulation of immune responses with an essential role in cancer immunity. Blockade of immune checkpoints especially PD-1/PD-L1 became a principle approach in cancer therapy. Different subtypes of Breast Cancer (BC) have different biological behaviors and distinct gene expression profiles. Moreover, microRNAs and Long-noncoding RNAs (LncRNAs) have gained wide spread attention recently as a critical key players in carcinogenesis but their underlying mechanisms remain limited. Our previous data revealed that overexpression of LncRNA; X inactive –specific transcript (XIST); negatively regulates PDL-1 in BC. Our work aims to investigate the impact of microRNAs and LncRNAs on PD-L1 in MDA-MB-231 cells, in addition to analyzing the differential expression of PD-L1 and non coding RNAs in different BC subtypes. Our data sheds the light on the differential expression of miR-182, MALAT-1 and XIST with a significant correlation to PD-L1 in TNBC and IDC. The data suggests a crucial role for these genes in the extent of evasion of cancer immunity in different BC subtypes.

  • Sessions:
    Genetic Engineering and rDNA Technology

    Time:

    Title: Insulation of Novel Nitrogen Starvation Induced Promoters in the Microalgae Phaeodactylumtricornutum

    Zachor Adler Agmon
    Ben-Gurion University,Israel

    Biography
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    Biography

    Zachor Adler Agmon
    Ben-Gurion University,Israel



    Abstract
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    Abstract

    Zachor Adler Agmon
    Ben-Gurion University,Israel

    The main aim of the research is to gain a better understanding of the biogenesis and movement of LDs in the diatom Phaeodactylumtricornutum. The lack of understanding of these processes in microalgae during oil accumulation requires the use of different molecular and biochemical approaches. The lack of nitrogen starvation induced promoters in the microalgae Phaeodactylumtricornutumdrove use to isolate novel nitrogen starvation induced promoters. We isolated and characterized several novel endogenous P. tricornutum gene promoters, two of which were found to induce gene expression during N depletion (Shemesh et al, US patent - ALGAL PROMOTERS). In order to demonstrate and test the applicability of those promoters, we overexpressed major LD protein of the green microalga Haematococcuspluvialis (HOGP) (Peled et al., 2011) in P. tricornutum fused with eGFP, under the control of the two novel N-starvation-inducible promoters DGAT1 and the ammonium transporter. Expression of eGFP-HOGP under N starvation induced localization of eGFP fluorescence to lipid droplets (LDs) after 3 and 7 days of starvation. HOGP expression under both the DGAT1 and the AT promoter resulted in significantly enhanced TAG accumulation of over 50%. The results of this study indicate the significant biotechnological potential of LD-associated protein genes in the metabolic engineering of microalgae for enhanced oil productivity (Shemesh et al., 2016).Based on the result, we are currently using those novel tools in order to develop a novel poultry vaccine using P. tricornutum as a cell factory for viral glycoproteins expression.

    Sessions:
    Marine Biotechnology

    Time:

    Title: Lipid Droplets biogenesis and Trafficking in the Microalgae Phaeodactylumtricornutum

    Zachor Adler Agmon
    Ben-Gurion University, Israel.

    Biography
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    Biography

    Zachor Adler Agmon
    Ben-Gurion University, Israel.



    Abstract
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    Abstract

    Zachor Adler Agmon
    Ben-Gurion University, Israel.

    The understanding and genetic engineering of algal lipid droplets (LD) biogenesis and trafficking hold great promise for the enhanced production of high value compounds or oil in microalgae. It seems that in P. tricornutum, the biogenesis of the LDs begins with several small, scattered LDs which gradually associate into one big LD. By using tubulin-specific cytoskeleton inhibitors, we found that this globule translocation and ultimate fusion can be inhibited. In addition, applying cytoskeleton inhibitors after relocating the LDs inside the cytoplasm, we saw a clear inhibitory effect of the microtubule inhibitor on the return of the LDs to their original location, which suggests that P.tricornutum LDs are connected to the microtubules. Further supporting evidence for this notion was found by tagging α tubulin and β actin with GFP, which demonstrated how the microtubules embrace the LDs, while the β actinis not come with any close interaction with the LDs. We have carry out proteomics on the LDs proteins of the P. tricornutum, to date, the majority of the proteins identified by it, are unknown proteins. Based on our need to isolated and identified proteins who have a role in LDs trafficking, we had to establish our won model; this model is based on the predicted 3D structure of a given protein, and its hypothetical interaction with the cytoskeleton. We will show in-vivo result that support our model. For example, we will show how the HOGP (Peled et al., 2011)is able to hijack the control over the cytoskeleton.

    Sessions:
    Pharmaceutical Biotechnology

    Time:

    Title: Polysaccharide Bacterial Toxins as Anticancer Agents

    Roger A Laine
    Rice University, USA

    Biography
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    Biography

    Roger A Laine
    Rice University, USA

    Professor Roger Laine, B.A., University of Minnesota, PhD, Rice University, Houston, Texas (Professor Alan D. Elbein), postdoctorals with Prof. Charles C Sweeley at Michigan State University, and Prof. Sen-itiroh Hakomori, University of Washington. He was Assistant and Associate Professor, University of Kentucky-Medical in Lexington, Kentucky, and then Professor and Chair of the Department of Biochemistry, Louisiana State University, currently Professor of Biochemistry and Chemistry. He was Chief Scientist, Glycomed, Inc., San Francisco, and Founder of biotechnology companies Anomeric, Inc., TumorEnd, LLC (www.tumorend.com), Citrazone, LLC, Enzomeric, LLC, and partner in Glycon, LLC, and has authored 150 publications, and 28 patents.



    Abstract
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    Abstract

    Roger A Laine
    Rice University, USA

    Busch, (1866, 1868), observed sarcoma patients with nosocomial erysipelas had spontaneous regression of tumors. Intentionally infection of sarcoma patients with erysipelas, showed regression of tumors in patients. Fehleisen, (1882) identified the erysipelas organism Streptococcus erysipelatos (S. pyogenes), repeated Busch’s observations, deliberately infecting cancer patients with injections of S. erysipelatos, finding tumor reduction in 7 of 7 cases. Bruns, (1888), described 3 out of 5 permanent cures of malignancies with erysipelas infections. Roger, (1892) in France, enhanced virulence of erysipelas streptococcus by co-injection in rabbits with Serratia marcescens,. W. B. Coley (1893, 1896, 1909, 1910) used heat killed cultures of both organisms commonly referred to as “Coley’s Toxin” for his treatments. “Coley’s Toxin” achieved clinical successes by Coley and others into the 1930's. In the 1980's Hellerqvist, Sundell, et al. isolated polysaccharide toxin from Group B Streptococcus, the causative agent of "Early Onset Disease" in humans. A potent 300kDa polysaccharide from GBS culture filtrate, was protein, LPS free containing lipid and phosphate. (Hellerqvist, 2002). GBS Toxin caused tumor specific capillary damage and tumor regression in rodents, and in a successful Phase I clinical trial in volunteer stage 4 humans (DeVore, et al. 1997), with 33% effectivity. A binding receptor 55kda protein (SP55) (Fu, et al., 2002) was found in humans encoded by the SLC17A5 gene. This receptor was found to be expressed on all tested human tumors in capillary endothelium. A Phase II trial is planned, and a canine cancer trial is in progress.

    Time:

    Title: Native Cell Membrane Nanoparticles System for Membrane Proteins

    Youzhong Guo
    Virginia Commonwealth University, USA

    Biography
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    Biography

    Youzhong Guo
    Virginia Commonwealth University, USA

    Youzhong Guo was born in Xiangcheng, Henan, China, in 1974. He received the B.S. degree in biology from Henan Normal University, Xinxiang, Henan, China, in 1997. He received the Ph.D. degree in pharmacy/structural biology from the University of Texas at Austin, Austin, TX, U.S.A., in 2010. From 2010 to 2016, he worked with Dr. Wayne A. Hendrickson as a postdoc in Columbia University, New York, NY, U.S.A. In 2016, he joined the Department of Medicinal Chemistry, Virginia Commonwealth University, Richmond, VA, U.S.A. as an Assistant Professor. His current research interests include membrane protein structural biology and structure-based drug discovery.



    Abstract
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    Abstract

    Youzhong Guo
    Virginia Commonwealth University, USA

    We devised a native cell membrane nanoparticles system, which we applied in a single-particle cryo-EM study of the multidrug exporter AcrB. Lipid-AcrB nanoparticles were prepared directly from membranes without any use of detergents. A 3D reconstruction in C1 symmetry achieved a final density map at 3.2 Å resolution, an atomic model of quasi-C3-symmetric AcrBwas fitted to this map, and the residual density revealed many ordered lipid molecules. Most remarkably, a central cavity between the three transmembrane domains contains a 24-lipid patch of well-ordered bilayer structure. Inner leaflet lipid chains pack in a hexagonal array like that in phosphatidylethanolamine crystal structures, whereas the outer leaflet has highly irregular packing. Protein side chains interact with both leaflets and participate in the hexagonal pattern. The AcrB export mechanism requires reorganization of the lipid bilayer structure. This system should be broadly applicable for membrane protein structural biology and structure-based drug discovery and development.

    Time:

    Title: Small Molecular Weight Peptides as Potential Therapeutics: Anticancer and Antimicrobial Effects of an Analogue to a Viral Protein

    Taghrid Istivan
    RMIT University, Australia

    Biography
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    Biography

    Taghrid Istivan
    RMIT University, Australia

    Dr. Istivan is a senior academic at RMIT University, Melbourne, Australia within the Biotechnology Discipline. Her research interests are in bacterial virulence, therapies and drug development. She was awarded a PhD in molecular microbiology in 2005 and has been affiliated with RMIT University as a researcher and a lecturer for 15 years. She has also supervised several PhD and Masters research projects within her research team and authored and coauthored research articles and book chapters in the fields of microbiology and novel therapeutics. She is currently the Biosciences senior program manager (Teaching and Learning) within the School of Science.



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    Abstract

    Taghrid Istivan
    RMIT University, Australia

    The resonant recognition model (RRM) can be employed to de novo design small molecular weight peptide analogues to known anticancer proteins, like viral proteins, interleukins, and tumor necrosis factor (TNFα). The biological effects of RRM-MV, an 18 aabioactive peptide analogue to myxoma virus NM-T5protein (AAC55050, 483 aa), were investigated on mammalian cell cultures and selected bacterial pathogens using qualitative and quantitative cell survival methods. Human apoptosis protein arrays were used to detect the levels of pro-apoptotic and anti-apoptotic proteins in treated cancer cells versus non treated cells. The small molecular weight peptide (2.3 kDa) produced cytotoxic effects on cancer cell lines including mouse melanoma (B16), human melanoma (MM96L), squamous cell carcinoma (COLO16), prostate cancer (PC3) and breast cancer (MCF7). Yet, no cytotoxic effects were detected on human red blood cells, skin fibroblasts and other normal mammalian cell lines. Furthermore, RRM-MV exhibited a bacteriostatic effect on Gram positive bacteria such as Staphylococcus spp. Furthermore, in treated cancer cells and prior to necrosis stage, the peptide was located inside cytoplasmic components predicted to be glycoproteins, and it also expressed different levels of binding specificity to glycans like Sialy Lewis X, gangliosides and mannoses when glycan arrays were performed to evaluate the binding affinity of RRM-MV. The data from this study indicated that RRM designed peptides such as RRM-MV have a potential to be developed as effective therapeutics, mostly for their ability to penetrate cellular membranes, and to interfere with specific cellular biological functions.

    Time:

    Title: Evaluation of the Pharmacological Profile of Virgin Coconut Oil (VCO) in Indomethacin-Induced Gastric Ulcer Models

    Parker Elijah Joshua
    University of Nigeria, Nigeria

    Biography
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    Biography

    Parker Elijah Joshua
    University of Nigeria, Nigeria



    Abstract
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    Abstract

    Parker Elijah Joshua
    University of Nigeria, Nigeria

    Several studies have reported various health benefits of virgin coconut oil (VCO) including its use for weight management, treatment of burns, various infections, and even HIV/AIDS. The present study was conducted to evaluate the pharmacological profile of VCO in indomethacin-induced gastric ulcer models. Twenty-four (24) Wistar albino rats were used for the study and were divided into 6 groups of 4 rats each. Group 1 rats served as the normal control; group 2 (positive control) rats were administered with indomethacin only, at a dose of 100 mg/kg b.w. Group 3 rats were treated with a standard drug (cimetidine) at a dose of 100 mg/kg b.w. Groups 4 (3 ml/kg b.w. VCO), 5 (6 ml/kg b.w. VCO) and 6 (9 ml/kg b.w. VCO) rats were treated as stated. The extract (VCO) and the standard drug were dissolved in distilled water, and were administered orally to the rats. Treatment with the standard drug (group 3) and VCO (groups 4, 5 and 6) lasted for four days, followed by indomethacin induction (groups 2 to 6) after an overnight fasting. Four (4) hours after induction, the rats were sacrificed and the blood samples collected for biochemical analyses. Stomach tissues were also harvested for histological examination. The gastric ulcer index of rats in groups 4 and 6 was found to be significantly (p < 0.05) higher compared to that of group 1, while group 5 rats treated with 6 ml/kg b.w. VCO, showed a non-significant (p > 0.05) increase in the gastric ulcer index compared to that of group 1. The ulcer protective indices of low, mid and high doses of the oil were found to be 38.71, 54.84 and 48.39 respectively, which were found to be higher compared to the standard control (6.45). Treatment with VCO resulted in a non-significant (p > 0.05) increase in the gastric juice volume of rats in groups 4, 5 and 6 compared to that of group 1. There was no significant (p > 0.05) decrease in the gastric pH of groups 4 and 5 rats when compared to the normal control. However, a significant (p < 0.05) decrease in the gastric pH was observed when a high dose of the extract was administered. Administration of the extract resulted in no significant (p > 0.05) differences in the lipid profile, lipid peroxidation and antioxidant parameters, compared to the normal control. Histological findings revealed that stomach sections of rats in groups 4 and 5 showed moderate widespread mucosal necrosis and ulceration, while that of group 6 rats showed focal area of mucosal ulceration with evidence of healing by fibrosis when compared to group 1. The findings of this research revealed that virgin coconut oil (VCO) possesses ulcer ameliorative properties and could therefore be used for the treatment of gastric ulcers. The pharmacological properties of virgin coconut oil could be anchored on mechanisms such as the ability to scavenge free radicals, stabilize cell membranes, stimulate mucous and prostaglandin secretion, inhibit Helicobacter pylori and modulate lecithin cholesterol acyltransferase (LCAT) activity.

    Time:

    Title: Bioactive Compounds from Rosmarinus Eriocalyx Growing in Algeria: Qualitative and Quantitative Characterization

    Bendif Hamdi
    Mohamed Boudiaf University, Algeria

    Biography
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    Biography

    Bendif Hamdi
    Mohamed Boudiaf University, Algeria



    Abstract
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    Abstract

    Bendif Hamdi
    Mohamed Boudiaf University, Algeria

    In an extensive search for bioactive compounds from plant sources, the composition of different extracts of R. eriocalyx stems, leaves and flowers collected from individuals spontaneously growing in North-East Algeria, was studied. The qualitative and quantitative characterization of rosemary (Rosmarinus eriocalyx) samples, obtained by two different solvents, i.e. water and ethanol. After 24 h extraction under magnetic stirrer, was determined by high performance liquid chromatography coupled to Diode-Array Detection (HPLC–DAD). A higher content of total polyphenols was found in the ethanolic extracts, the leaves were the richest part, followed by flowers and stems. On the other hand, aqueous extracts of R. eriocalyx showed a significant lower level of polyphenols in all parts, The major compound present in the ethanolic extracts was rosmarinic acid, that instead was present in poor amounts in the water extracts. The same trend is showed by carnosic acid in ethanolic extracts and carnosol in ethanolic extracts. Leaves were the richest part in terms of rosmarinic acid, carnosic acid and carnosol.

    Sessions:
    Biotechnology in Healthcare

    Time:

    Title: The Casein Kinase 2 (CK2) and PIm Kinase as a Target for Anticancer therapy

    Maria Bretner
    Warsaw University of Technology, Poland

    Biography
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    Biography

    Maria Bretner
    Warsaw University of Technology, Poland

    Maria Bretner received M.S. degree in chemistry from the Faculty of Chemistry , University of Warsaw in 1974 and Ph.D in biological sciences from the Institute of Biochemistry and Biophysicsin 1997.She completed postdoctoral studies at the University of Maryland Baltimore County, USA. (1997-1999). She worked at the IBB PAS developing the methods of new inhibitors synthesis of Thymidylate Synthase, HIV RT andHCV helicase. Since 2007 she is working at the Warsaw University of Technology,Faculty of Chemistry .Research areas include chemistry andenzymology, biocatalysis, biochemistry of the cancer processes. Co-author of 69 scientific papers.



    Abstract
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    Abstract

    Maria Bretner
    Warsaw University of Technology, Poland

    Protein kinases CK2 and PIM belong to serine/threonine kinases that are involved in the regulation of number of signalling pathways. They are upregulated in multiple cancers, including lymphoma, leukemia, breast, prostate, head and neck cancers and act as repressors of apoptosis, contributing to chemoresistance. Over 30 drugs, that target kinases, have been approved for clinical use over the past decade, and hundreds more are undergoing clinical trials, among them are: specific inhibitor of CK2-CX4945 and few specific inhibitors of PIM family e.g. AZD1208, CX-4595, SGI-9481 or LGB321. But their efficiency is not satisfactory so the increasing attention has been recently focused on the significant role of dual kinase inhibitors. Until now a few examples of the benzimidazole derivatives, which affect the activity of both kinases, CK2 and PIM1 have been known. We designed and synthesized novel 4,5,6,7- tetrabromo-1H-benzimidazole (TBBi) and 4,5,6,7- tetrabromo-1H-benzotriazole (TBBt) derivatives with alkylamine substituents. To test the inhibitory properties recombinant human kinase CK2α, CK2 holoenzyme, and kinase PIM1 was obtained in E. coli bacterial expression system. The most promising compound, the 3- (4,5,6,7-tetrabromo-2 methyl-1H-benzimidazol-1-yl) propan-1-amine (14b) inhibited the activity of CK2 with an IC50 of 0.35 micromolar and PIM1 0.15 micromolar. Furthermore, the influence of new active dual inhibitors on the cell viability was evaluated and EC50 determined for 14b with the use of CCRF-CEM, MCF-7 and PC3 cell lines were in the range of 2-4 micromolar. This work was supported by an NSC Poland grant 2014/13/B/NZ7/02273 and by WUT.

    Time:

    Title: Engineering Bugs for the Discovery of New Drugs Against Neurodegenerative Diseases

    Georgios Skretas
    National Hellenic Research Foundation, Greece

    Biography
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    Biography

    Georgios Skretas
    National Hellenic Research Foundation, Greece

    Georgios Skretas graduated from the School of Chemical Engineering of the National technical University of Athens (Greece) in 1998 and received his PhD in Chemical Engineering from Princeton University (USA) in 2006. He then moved on to the University of Texas at Austin (USA) to carry out post-doctoral research training under the guidance of Prof. George Georgiou. Since 2009, Dr. Skretas has been the principal investigator of the Laboratory of Enzyme & Synthetic Biotechnology at the Institute of Biology, Medicinal Chemistry & Biotechnology of the National Hellenic Research Foundation (Greece), where he currently holds the rank of Research Associate Professor.



    Abstract
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    Abstract

    Georgios Skretas
    National Hellenic Research Foundation, Greece

    Protein misfolding is a common pathological feature for many human diseases, such as Alzheimer’s disease (AD), Parkinson’s disease, type II diabetes and others. We will describe the development and application of an integrated and generalizable bacterial platform for facile discovery of macrocyclic rescuers of disease-associated protein misfolding. In this system, large combinatorial libraries of macrocycles are biosynthesized in Escherichia coli cells and simultaneously screened for their ability to rescue pathogenic protein misfolding using a genetic assay based on fluorescence-activated cell sorting. We will first describe the effectiveness of this approach through the identification of drug-like, head-to-tail cyclic peptides that modulate the aggregation of the amyloid β peptide (Aβ) of AD. By using a series of biochemical, biophysical and biological assays using isolated Αβ, primary mammalian neurons and various established AD models in the nematode Caenorhabditis elegans, we have found that the selected macrocycles potently inhibit the formation of neurotoxic Αβ aggregates. Further, to showcase the generality of our approach, we will describe the application of the same platform for the identification of misfolding rescuers of mutant Cu/Zn superoxide dismutase 1 (SOD1), a protein whose misfolding and aggregation is associated with inherited forms of amyotrophic lateral sclerosis. Overall, our approach represents a straightforward strategy for the discovery of molecules that rescue the misfolding of polypeptides known to be associated with disease effectively.

    Time:

    Title: Curcumin Reduces Antioxidant Activity Following Spinal Cord Injury in Rat Model

    Ahmed Abdellatif
    The American University in Cairo, Egypt

    Biography
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    Biography

    Ahmed Abdellatif
    The American University in Cairo, Egypt

    Ahmed Abdellatif is an assistant professor of biology at the School of Sciences and Engineering, The American University in Cairo, Egypt. He received an MSc and a PhD from the University of Louisville, School of Medicine in Anatomical Sciences and Neurobiology. He also earned an MBBCH and a Master of Science in anatomy and embryology from Alexandria University School of Medicine. Before joining AUC, he was involved in curriculum design for medical education and taught medical and graduate level courses in human gross anatomy, neuroscience. Dr. Abdellatif has been interested in drug delivery and the development of cost effective treatments using natural herbal extracts for various applications.



    Abstract
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    Abstract

    Ahmed Abdellatif
    The American University in Cairo, Egypt

    Spinal cord injury is a debilitating disability. Oxidative damage and inflammation are two hallmarks of the secondary spinal cord injury. The objective of this study was to evaluate the potential of Curcumin (a polyphenolic compound extracted from the rhizome of Curcuma longa that has been known to possess antioxidant and anti-inflammatory properties) as an antioxidant and anti-inflammatory agent following spinal cord injury in rats, and to compare its therapeutic effects following local application directly to the injury versus its oral dietary supplementation in a spinal cord hemisection model at T9-T10. Female Sprague Dawley rats were randomized into a control, injury, and treatment groups of local single dose of Curcuma longa extract immediately on the injury site and a Dietary supplement group. Crude Curcumin was added to the animals’ feed (10% of daily feed) one week before and week after injury. Oxidative stress parameters were Malondialdehyde (MDA) and total antioxidant capacity (TAC). Expression of tumor necrosis factor alpha (TNF α) and interleukin 6 (IL-6) was detected using Enzyme Linked Immunosorbent Assay (ELISA). Our results show that at 7 days, although Dietary supplement was effective in increasing TAC levels and lowering TNF αexpression levels, it did not affect MDA levels. Local treatment regimen has shown to be more effective on all four parameters. Our results demonstrate that local Curcumin application directly on the injury site might be more efficacious in alleviating oxidative damage and reducing inflammation following spinal cord injury.

    Time:

    Title: Continuous Multilayered Composite PVA Hydrogel as Cartilage Substitute

    Gemma Leone
    University of Siena, Italy

    Biography
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    Biography

    Gemma Leone
    University of Siena, Italy

    Gemma Leone is a researcher at the University of Siena. The research activity is focused on the synthesis and the physico-chemical and rheological characterization of natural or synthetic macromolecular based bi-dimensional or three-dimensional surfaces, which can be utilized as cell scaffolds or drug vehicles. Her research fields mainly concern on : I) Development of new polysaccharide –based hydrogels for soft tissue regeneration II) Protein adsorption studies on biomaterials III) Synthesis and physico-chemical and rheological characterization of polyvinylic hydrogels as ophthalmological devices IV) Development of model systems for saccharide based biosensors.



    Abstract
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    Abstract

    Gemma Leone
    University of Siena, Italy

    Cartilage is a highly organized avascular soft tissue that assembles from nano-to macro-scale to produce a complex structural network. To mimic cartilage tissue, we developed a stable multilayered composite material (MSC), characterized by a tailored gradient of mechanical properties. MSCwas obtained through a multistep procedure. A mixture of PVA and HA nanocrystals (nHA/PVA molar ratio of 0.015) was crosslinked using tri-sodium tri-metaphosphate (STMP), the crosslinking agent, added in a molar ratio 1:1 with PVA (PS11HA)(first layer). The second layer was obtained by crosslinking PVA directly on the surface of first layer without addition of the inorganic phase but with the same PVA/STMP molar ratio of the first layer (PS11). The same procedure was then applied to crosslink a third layer, which was produced with a greater PVA/STMP molar ratio (2:1). MSC can be considered a promising potential substitute for damaged cartilage tissue, since it mimics the gradient of water content and rheological properties strictly comparable with those of cartilage in terms of complex modulus (G*: 0.032±0.003 MPa; cartilage: G*: 0.03±0.003 MPa) and recovery (70% recovery after just 0.1 s). The presence of nano-hydroxyapatite in its bottom layer stimulate the adhesion to bone, whereas the uppermost soft layer represents an ideal environment for interaction with cartilage guaranteeing a lubricant action as confirmed by the good cytocompatibility shown by MSC (layer PS21) and MSC (layer PS11HA) towards chondrocytes and osteoblasts, respectively, and by the increased BALP production in samples containing nHA in comparison with samples without nHA.

    Time:

    Title: Medical Pluralism- Practice of Alternative Medicine in Rural Communities of Nepal

    Himal Luitel
    Agriculture and Forestry University, Nepal

    Biography
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    Biography

    Himal Luitel
    Agriculture and Forestry University, Nepal

    Himal Luitel is an academician and researcher, currently serving as Assistant Professor in Center for Biotechnology, Agriculture and Forestry University, Chitwan, Nepal. He is veterinary graduate from Tribhuvan University, Nepal, Masters in Molecular Biology from Interuniversity Program Molecular Biology, Belgium, and PhD, Post-Doctorate from Excellence Cluster Cardio-Pulmonary System, Justus-Liebig University, Germany. He has expertise in in vivo (lab animals) and in vitro testing of pharmacologically active compounds. Currently, he is establishing new lab in Center for Biotechnology, Agriculture and Forestry University, Chitwan, Nepal where he is planning to do research in natural products in laboratory animals and in vitro settings. Currently, he is conducting research on common bacterial diseases of poultry for development of rapid molecular diagnostic tools and identify candidate genes for vaccine development.



    Abstract
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    Abstract

    Himal Luitel
    Agriculture and Forestry University, Nepal

    Alternative medicine has been practiced and still widely accepted in Chinese as well as south Asian medical system. Use of both complementary and alternative medicine (CAM) is quite popular in rural Nepal where people are using both alternative as well as allopathic medicine. Several plant, animal and other natural resources are widely used as either therapeutic options or nutritional supplements. Owing to the huge geographical diversity of Nepal, it is rich in flora and many of them have medical importance. There is not systematic study of Nepalese flora in terms of active ingredients and their medicinal properties. Efficacy of Yarsagumba and its bioactive compound cordycepin in Pulmonary Hypertension (PH) was tested by well-designed systematic experimental setting in cell culture as well as murine model. Entomogenous fungus Yarsagumba (Cordycepssinensis) and its biochemically active constituents such as cordycepin, which have among others the anti-oxidant, anti-inflammatory and vasodilatory effects could be the therapeutic options for this incurable disease. We investigated the effect of Yarsagumba extract and its purified bioactive compound cordycepin on human pulmonary artery smooth muscle cells (hPASMCs) and in murine model of isolated lungs to investigate their still unknown anti-proliferative and vasorelaxant properties in the context of Pulmonary Hypertension (PH). Both Yarsagumba extract and cordycepin significantly reduced proliferation of hPASMCs derived from donor and PH subjects. However, only Yarsagumba extract, and not cordycepin, showed the vasodilatory outcome, suggesting the existence of other active metabolites present in Yarsagumba which may be responsible for this effect. Further research in the field is needed to provide the detailed and mechanistic insights about the exact therapeutic potency of Yarsagumba extract and whether it can be used as an option to treat PH patients.

    Time:

    Title: Health Disparities in Females with Rheumatic Heart Disease in Central Afghanistan

    Ruhina Najem
    Walden University, USA

    Biography
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    Biography

    Ruhina Najem
    Walden University, USA



    Abstract
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    Abstract

    Ruhina Najem
    Walden University, USA

    In developing countries, and sporadically in developed countries, Rheumatic Heart Disease (RHD) persists in being the main health condition, and yet the medical management of RHD throughout the years has not changed. The acquisition of RHD as well as its pathogenesis remains elusive. What is known is that Anonsuppurative indication of group A streptococcal (GAS) pharyngitis causes rheumatic fever (RF) which develops into RHD when untreated. Secondary prophylaxis proves ineffective in preventing death from this disease. Because an anti-streptococcal vaccine does not yet exist, ancillary prophylaxis is not possible (Vijayalakshmi, 2011). The attempt to invent such a vaccine using the M protein has been unsuccessful over the last 40 years (Vijayalakshmi, 2011). Although developing countries have reduced the incidence of RHD, the disease still remains one of the leading causes of mortality and morbidity in developing countries worldwide. This study offers strategies to eliminate health disparities in women with RHD in Central Afghanistan, where the life expectancy for women is 44 years old. By increasing the number of trained healthcare professionals, as there are presently 695 female doctors for 4,595,000 women, and by increasing the health quality of life in a region where 85% of women are illiterate, the incidence of RHD in women in Central Afghanistan can be significantly reduced (Arnold, Teijlingen, Ryan, & Holloway, 2014; Trust In Education. 2011; Bezruchka, 2012).

    Time:

    Title: Development of Low Cost Nano Bioadsorbent Composite Materials for Pharma Waste Treatment

    Lingayya Hiremath
    R.V College, India.

    Biography
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    Biography

    Lingayya Hiremath
    R.V College, India.

    Dr. Lingayya Hiremath, had awarded Ph.D in Biotechnology in year 2004 from Gulbarga University, Gulbarga. He is currently working as Asst. Professor, Dept. Biotechnology, R.V. College of Engineering, Bengaluru, and Karnataka India. He received Young scientist state award from VGST, Govt. of Karnataka in year 2014. He had published more than 20 research papers in reputed national and International journals. He given invited talk in National and International forms such as UB Technological University, Prague Czech Republic. He has been guiding UG/M.Tech and Ph.D students in the various areas in Biotechnology. He has research interest in the field of Environmental biotechnology, Nanotechnology and Clean Energy Technology. He had received funds from various funding agencies to carryout research activities. Recently, he received outstanding Project of the year award from Karnataka State Council for Science and Technology, Indian Institute of Science Bengaluru. He had received Funds to setup R&D Centre on Waste to Clean Energy Lab by Karnataka Council for Technological Upgradation at R.V. College of Engineering, Bangalore.



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    Abstract

    Lingayya Hiremath
    R.V College, India.

    Medication disposal is alarming issue today and gaining more and more awareness from the healthcare professionals as well as consumers. Pharmacists have the potential to be on the forefront of this movement as healthcare professional and pharmacists are in admirable position to educate patient about safe drugs disposal. Proper patient counseling on safe medication disposal can make a significant difference to public health and environment. A practical approach should be there to incorporate this important issue in the curriculum as the need of the hour. Also establishment of cost-effective and acceptable government run collection and disposal systems is necessary. There should be some norms and stringent guidelines for same. Careful and proper disposal of medications can help to decrease environmental load of drugs. The role of biotechnology for disposal and treatment techniques for medical waste at cost-effective methods will be discussed. All multidisciplinary stake holders, governments, NGOs, physician, pharmacist, patients and public should work together hand in hand to reduce burden of unused and expired medicines on ecosystem. Scientific waste management strategy is needed to ensure health and environmental safety.

    Time:

    Title: Study of Translational Gaps to Address the Affordable and Accessible Priority Indigenous Technologies: Emerging Aid to India's Health Sector

    Smita Sahu
    Amity University, India.

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    Biography

    Smita Sahu
    Amity University, India.



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    Abstract

    Smita Sahu
    Amity University, India.

    Medical Devices and Diagnostics cater an integral component of the health care system with a mandate of 'access and equity'. India is battling with the issues of accessibility, affordability and availability of quality health for Public. Indian medical heritage dated back to 3000BC unveils the rich knowledge pool which has undergone a perceptible change over years, such as eradication of many Communicable diseases, changing statistics of individual awareness of quality health and import driven medical device market etc. Despite slew of initiatives the holistic slogan of health for all remains the concern of nation. The present scenarioexhibiting demographic dividend and geographical segmentation fostering varied needs of people as per their regional conditions of climate, disease prevalence, nutrition and sanitation requires reinforcing the potential of indigenous technologies in coordination with prevailing health issues in sectoral/regional/territory of country. This paper proposes to adopt technology transfer as a strategy to establish a vibrant ecosystem for identifying and upscaling the indigenous medical technologies with diligent handholding to address the diagnostic capacity building with robust regulatory regime to mitigate the challenges of accessibility in resource poor settings, import dependency in the conducive promising initiative of government like Make- In-India, which opens new avenues to a flourishing future of indigenous medical health technologies and innovations for delivering affordable healthcare to India's billion-plus population.

    Sessions:
    Poster Presentations

    Time:

    Title: Solid-Phase Enzyme Catalysis 0f DNA End Repair Avoids Heat Treatment and Reduces GC-Bias in Next-Generation Sequencing of Human Genomic DNA

    Aihua Zhang
    New England Biolabs, USA

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    Biography

    Aihua Zhang
    New England Biolabs, USA



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    Abstract

    Aihua Zhang
    New England Biolabs, USA

    Next-generation sequencing (NGS) has caused a revolution in both research and diagnosis. NGS analysis relies on preparation of a representative, non-biased library evenly distributed across the entire genome under investigation. This critical task has become increasingly challenging since biases found in the current methods of the NGS library preparation produce uneven coverage and compromisethe quality of NGS analysis. In this report we have identified a systematic sequence bias during construction of amplification-free human DNA librariesfor the Illumina sequencing platform. Our study indicatesthat inefficient processing of AT-rich DNA in the major steps that comprise library construction resultsin under-representation of the extremely high AT-content fraction in human genomic libraries. We have demonstrated a new strategy by employingDNA modifying enzymes conjugated to magnetic beads in construction of amplification-free human DNAlibraries. We show that this method significantly lowers the sequence coverage bias of the libraries on Illumina sequencing platform.

    Time:

    Title: Native Cell Membrane Nanoparticles System for Membrane Proteins

    Weihua Qiu
    Virginia Commonwealth University, USA.

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    Biography

    Weihua Qiu
    Virginia Commonwealth University, USA.



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    Abstract

    Weihua Qiu
    Virginia Commonwealth University, USA.

    We devised a native cell membrane nanoparticles system, which we applied in a single-particle cryo-EM study of the multidrug exporter AcrB. Lipid-AcrB nanoparticles were prepared directly from membranes without any use of detergents. A 3D reconstruction in C1 symmetry achieved a final density map at 3.2 resolution, an atomic model of quasi-C3-symmetric AcrB was fitted to this map, and the residual density revealed many ordered lipid molecules. Most remarkably, a central cavity between the three transmembrane domains contains a 24-lipid patch of well-ordered bilayer structure. Inner leaflet lipid chains pack in a hexagonal array like that in phosphatidylethanolamine crystal structures, whereas the outer leaflet has highly irregular packing. Protein side chains interact with both leaflets and participate in the hexagonal pattern. The AcrB export mechanism requires reorganization of the lipid bilayer structure. This system should be broadly applicable for membrane protein structural biology and structure-based drug discovery and development.

    Time:

    Title: Microbial Production of Natural 2-Phenylethanol

    Karolina Chreptowicz
    Warsaw University of Technology, Poland.

    Biography
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    Biography

    Karolina Chreptowicz
    Warsaw University of Technology, Poland.

    Karolina Chreptowicz is currently a Ph.D. candidate at the Warsaw University of Technology. She earned her Bachelor and then Master degree in Industrial Biotechnology at the Warsaw University of Technology, Faculty of Chemistry, Poland. Since 2013, she has been working with dr Jolanta Mierzejewska in the field of yeast biotechnology. At present, in her Ph.D. thesis, she is involved in the development of a laboratory-scale technology for the production of natural 2-phenylethanol - starting from the biotransformation stage by separating and purifying the final product.



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    Abstract

    Karolina Chreptowicz
    Warsaw University of Technology, Poland.

    Thanks to its pleasant rose flavor as well as antibacterial and antifungal properties, 2-phenylethanol (2-PE) has huge market demand. After vanillin, it is the second-most-used additive in perfumery, while also being a component in the food and pharmaceutical industries. Although nowadays most 2-PE originates from chemical synthesis, biotechnological production with yeast is becoming increasingly more attractive since it gives a final product classified as natural. The presented work is focused on microbial production of natural 2-PE. The first stage of the study was the search for yeast strains, isolated from the natural environment, that will efficiently produce 2-PE. With the best producer, we have developed a complete technology of 2-PE production - starting from the biotransformation stage and ending with pure product. In a batch culture conducted in a 5-l bioreactor, we obtained 3.6 g/l 2-PE after 72 h. As 2-PE titer of 2-4 g/l in broth is toxic for yeast, we achieved maximum concentration in a simple batch-culture. Therefore, to enhance productivity we tested extractive fermentation as one of several in situ product removal (ISPR) techniques. We showed for the first time that rapeseed oil can be successfully applied for this purpose. In addition, it is also an excellent biomaterial with promising use in the food or cosmetic industries. Recently, our work has focused on reducing the production costs and, to this end, we tested organic waste from agriculture and food processing as cheap feedstock. This approach has two advantages: lowering the price of the culture medium and better management of harmful wastes. Currently, we are working on downstream processing to combine all stages in one complete technology. This work was financially supported by the National Science Centre, Poland (2016/21/D/NZ9/01605).

    Time:

    Title: Determination and Quantification of Heavy Metals using Infrared Spectroscopy and Chemometric Techniques

    Raul Delgado Macuil
    CIBA, IPN, Mexico

    Biography
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    Biography

    Raul Delgado Macuil
    CIBA, IPN, Mexico

    Raul Delgado Macuil born in Puebla Mexico. He received B.S. degree in electronics from BUAP by Physical-Mathematics School in 1994; and he received the PhD in Optics from Astrophysical Optics y Electronics National Institute in 2005; both institutions in Mexico. Head research in the nanobiotechnology and biosensors fields in the Applied Biotechnology Research Center, a National Politechnique Institute center in Mxico. As author or co-author, about 70 scientific and professional papers have been published. Head of 21 national projects and more than 25 graduate and postgraduate thesis has been directed, have more than three thousand nationals and international congress participations.de Puebla, 1994; MA, Optoelectronics,Benemrita Universidad Autnoma de Puebla 1996; PhD, Physical Optics, InstitutoNacional de Astrofsica, ptica y Electrnica, 2005, Research Interests Biosensors, vibrational spectroscopy (Infrared and Raman); 21 national Grants, seven external to IPN and fourteen institutional; Thesis as director Bachelor: 10, Master in Sciences: 13 and PhD: 5; 30 JCR Publications (110 cited publications); one national patent, four book chapters, more than three thousand nationals and international congress participations.



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    Abstract

    Raul Delgado Macuil
    CIBA, IPN, Mexico

    Actually the determination and quantification of heavy metals in food has a high priority for public health, quality systems and food safety, these elements can be added to the food production system at different stages of the agri-food chain and it is essential to monitor his presence or absence through it. Until now, the methodology to perform this task is based on atomic absorption spectrophotometry that requires at least a couple of days to obtain a reliable result. The aimed of this work is develop and validate an alternative analytical method for the determination of heavy metals in several matrices, based on numerical methods and the correlation between spectrometric techniques; Atomic Force and Infrared by Fourier transform. The presence of Hg and Pb at different concentrations seem to modify the milk spectrum in the region of proteins, lipids and OH; at 1636, 1337, 550, 3300 and 3350 cm-1.The presence of As, Cd, Cr Hg and Pb at concentrations even of 0.1 ppb generate characteristic IR spectra that can help to identify their presence in water. By Principal Components Analysis (PCA), was possible have a better discrimination of the samples in the same matrix (milk).Where the cloud of points in the different regions of interest, show a good discrimination for the seven metals used in this work.

    Time:

    Title: The Mechanism of Action of Zingerone in the Pacemaker Potentials of Interstitial Cells of Cajal in Murine Small Intestine

    Byung Joo Kim
    Pusan National University School of Korean Medicine, Republic of Korea

    Biography
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    Biography

    Byung Joo Kim
    Pusan National University School of Korean Medicine, Republic of Korea



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    Abstract

    Byung Joo Kim
    Pusan National University School of Korean Medicine, Republic of Korea

    Background: Zingerone, a major component found in ginger root, is clinically effective for the treatment of various diseases. Interstitial cells of Cajal (ICCs) are the pacemaker cells responsible for slow waves in the gastrointestinal (GI) tract.We investigated the effects of zingerone on the pacemaker potentials of ICCs to assess its mechanisms of action and its potential as a treatment for GI tract motility disorder. Methods: We isolated ICCs from small intestines, and the whole-cell patch-clamp configuration was used to record the pacemaker potentials in cultured ICCs. Results: Under the current clamping mode, zingerone inhibited pacemaker potentials of ICCs concentration-dependently. These effects were blocked not by capsazepine, a transient receptor potential vanilloid 1 (TRPV1) channel blocker, but by glibenclamide, a specific ATP-sensitive K+channel blocker. Pretreatment with SQ-22536 (an adenylate cyclase inhibitor), LY294002 (a phosphoinositide 3-kinase inhibitor), and calphostin C (a protein kinase C (PKC) inhibitor) did not block the effects of zingerone on the pacemaker potentials relative to treatment with zingerone alone. However, zingerone-induced pacemaker potential inhibition was blocked by 1H [1,2,4] oxadiazolo [4,3 a] quinoxalin 1 one (ODQ; a guanylate cyclase inhibitor), KT5823 (a protein kinase G (PKG) inhibitor), and L-NAME (a non-selective nitric oxide synthase (NOS) inhibitor). In addition, zingerone stimulated cyclic guanosine monophosphate (cGMP) production in ICCs. Finally, pretreatment with PD98059 (a p42/44 mitogen-activated protein kinase (MAPK) inhibitor), SB203580 (a p38 MAPK inhibitor), and SP600125 (c Jun N terminal kinases (JNK) specific inhibitor)blocked the zingerone-induced pacemaker potential inhibition. Conclusion: These results suggest that zingerone concentration-dependently inhibits pacemaker potentials of ICCs via NO/cGMP-dependent ATP-sensitive K+channels through MAPK-dependent pathways. Taken together, this study shows that zingerone may have the potential for development as a GI regulation agent.

    Time:

    Title: Analysis of the Expression of Three Genes Involved in the Biosynthesis of Anthocyanin Using RT-qPCR in Rubus Niveus

    Viviana Chiluisa-Utreras
    Politecnica Salesiana University, Ecuador.

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    Biography

    Viviana Chiluisa-Utreras
    Politecnica Salesiana University, Ecuador.



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    Abstract

    Viviana Chiluisa-Utreras
    Politecnica Salesiana University, Ecuador.

    Biotechnological applications such as (RT-qPCR) Quantitative Polymerase Chain Reaction,are laboratory techniques that have revolutionized the world of Molecular Biology, providing results in less time and a high degree of reliability, allowing the understanding of genes, functioning and expression, as in the case of the genes of the anthocyanins, which in recent times have increased their interest due to its antioxidant activity which generates therapeutic effects such as reduction of coronary heart disease, improvement of visual acuity, anti-cancer, anti-aging and antitumor effects. The Polymerase Chain Reaction with reverse transcription (RT-qPCR) allowed to analyze the level of expression of β-Actin, RuANS and RuMYB10, genes involved in the biosynthesis of anthocyanin in Rubus niveus, collected in three different zones of Rumiñahui-Ecuador. According to values obtained with statistical analyses such as Shapiro Wilks, Anova and Tukey test, it was noted that significant differences in the expression of three genes didn´t exist and there were generated the following results: gene β-Actin, which has greater concentration (174, 65ngmL), followed by the RuANS gene (167, 43ngmL) and finally the RuMYB10 gene (163, 55ngmL), reaching the conclusion that the studied species presents a level of similar expression among the three analyzed genes. This research can be considered as a starting point of projects for improving the genus Rubus(blackberry), in order to increase their nutritional value moreover grow them as a source for the production of nutraceuticals, drugs and therefore developing productivity and economic value that this crop representsfor the country.

    Time:

    Title: Affinity Protein Purification Resulting in Protein Sequence without Remaining Amino Acid Residues

    Heba Alaa Eldeen Hosiny Abd Elhameed
    University of Szeged, Hungary

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    Biography

    Heba Alaa Eldeen Hosiny Abd Elhameed
    University of Szeged, Hungary

    HebaAlaaEldeenHosinyAbdElhameedis a PhD student in the University of Szeged.She is from Egypt, Where she has been working as Assistant lecturer atZagazig University.Presently she is examining new types of artificial nucleases by means of various biophysical methods within the frame ofa StipendiumHungaricumPhD scholarship and under supervision of Dr. Bela Gyurcsik.



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    Abstract

    Heba Alaa Eldeen Hosiny Abd Elhameed
    University of Szeged, Hungary

    Purification of proteins is vital for the characterization of the function, structure and interactions of the protein of interest and efficiently carried out by affinity chromatographic methods. The specific interaction with the appropriate resins requires fusion affinity tags, such as e.g. the oligo-His, the maltose binding protein or glutathione-S-transferase tag. These sequences are encoded by the plasmids used for protein expression. The affinity tags have to be cleaved off after the target protein is selectively bound to the solid support. This is performed by specific proteases. These enzmes are expensive and mostly they leave few extra amino acids at the terminus, which may interfere with the structure and function of the purified protein. Recent studies in our research group focused on the protein constituents of new artificial nucleases. Artificial DNA nucleases have provided scientists with the unprecedented ability to probe, regulate, and manipulate the human genome. Zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and the clustered regularly interspaced short palindromic repeat-Cas9 system (CRISPR/Cas9) represent a powerful array of tools that can bind to and cleave a specified DNA sequence. Our ressearch focused on new type of ZFNs with intramolecular alloteric activation. The computer design of such enzymes requires the precise protein sequence to be obtained. Using the Ni(II)-affinity chromatography for e.g. zinc finger protein purification requires the complete removal of the oligo-His affinity tag. However, this was not possible to achieve by the traditional methods. We redesigned the cloning region of pET21a plasmid. The new approach is to cleave the His-tag by Ni(II) instead of proteases. Therefore, the new plasmid encodes the affinity tag with a Ni(II)-sensitive cleavage site at its N terminus. The precise gene of the protein is inserted into the cloning region by the help of BsmBI restriction endonuclease, so that the recognition site is deleted in the cleavage/ligation procedure. We performed the cloning, expression and purification procedures and will report the results on the poster.

    Time:

    Title: Determination of Optimal Sterilization Types for In Vitro Propagation of Walnuts Cultivars in Georgia

    Iveta Megrelishvili
    Georgian Technical University, Georgia

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    Biography

    Iveta Megrelishvili
    Georgian Technical University, Georgia

    IvetaMegrelishvili has completed his PhD at the age of 28 years from IvaneJavakhishvili Tbilisi State University. She is the main research scientist of Georgian Technical Univeristy, Biotechnology Center and Head of Virology Lab, Scientific-Research Center of Agriculture. He has published more than 8 papers in reputed journals and has a great experiences in the field of plant biotechnology, plant virology and molecular biology.



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    Abstract

    Iveta Megrelishvili
    Georgian Technical University, Georgia

    Microbial diseases have been reported in the walnut orchards due to different reasons in recent years in Georgia. For the purpose of walnuts tissue cultures reproduction, it is necessary to determine the optimal type of sterilization of initial material. The aims of this study were determined optimal sterilization type to propagate walnuts cultivars ‘Pedro’, and ‘Chandler’ using tissue culture technique. The initial explants (unimodal micro cuttings with a length between 1 and 1.2 cm) of three cultivars were collected from the young 2-3 years old walnuts orchards, Dzevera, ShidaKartli, Georgia. Two sterilized types were used for in vitro propagation of walnuts: I.1-2% hypochlorite 10-15 min, followed 70 % alcohol -30 min and 3 times sterile distillate water II. 0.1% mercuric chloride 5 min and 3 times sterile distillate water. It was revealed that microbial contamination were 58.97% using 0.1% mercury chloride and relatively high by sodium hipocloride-85.12%. DKW medium was used for in vitro cultivation of walnuts cultivars supplemented with 0.1 mg/L IBA, 1 mg/L BAP and sucrose 3%. The pH of culture medium was adjusted at 5.5 before adding the gelling agent and autoclaving. Micro cuttings were kept at 25±2oC under a 16 h photoperiod. Finally, optimal sterilization types of initial explants (Combination II) was determined. Walnuts cultivars: ‘Pedro’ and ‘Chandler’ were propagated using tissue culture methods first time in Georgia. This study led to develop an effective method for micro propagation of Juglansregia, which enable us to establish new walnuts orchards in Georgia.

    Time:

    Title: Honey as a Strong Competitor in the Biosynthesis of Silver Nanoparticles and Promising of its Antibacterial Activity

    Ghada Youssef
    Alexandria University, Egypt.

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    Biography

    Ghada Youssef
    Alexandria University, Egypt.



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    Abstract

    Ghada Youssef
    Alexandria University, Egypt.

    This research article represents a strong approach of green synthesis of silver nanoparticles (AgNPs) using a local bio-derived product-honey called black seed honey (BSH). Antioxidant activity of BSH had been determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and found to be 91.34% scavenging activity. Antibacterial activity of BSH and AgNPs was investigated against six pathogenic strains; Escherichia coli, Proteus mirabilis, Klebsilla pneumoniae, Pseudomonas aeruginosa (Gram-negative), Staphylococcus aureus, Streptococcus mutans (Gram-positive). BSH can reduce silver ions into silver nanoparticles after 20 min with stirring the reaction mixture. Nanoparticles of different sizes could be obtained and the solution turned to black by time. Characterization of the AgNPs was done by UV-Visible Spectroscopy, Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM) and scanning electron microscope (SEM) imaging. The colloid obtained at a pH of 9 was found to be spherical in shape distributed with some agglomeration. An intense surface plasmon resonance band at 400 nm in the UVvisible spectrum clearly revealed the formation of AgNPs after 72h. Transmission electron microscopy (TEM) showed spherical shaped of AgNPs, and the size of nanoparticles was in range of 25 ~70 nm. The results suggest that the synthesized AgNPs act as an effective antibacterial agent more than natural black seed honey, and can potentially be used in human contacting areas.

    Time:

    Title: Effect of Nitrogen and BAP in the Tissue Culture of Lupinuspubescensbenth, an Emblematic Quito Plant

    Ivonne Vaca Suquillo
    Politecnica Salesiana University, Ecuador

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    Biography

    Ivonne Vaca Suquillo
    Politecnica Salesiana University, Ecuador

    Ivonne Vaca Suquillo, with studies in Agricultural Engineering and Masters in Agricultural Biotechnology. With 10 years of experience in the development of plant tissue culture, in different species, among these Andean fruits, tropical fruits, ornamental plants, plants of conservationinterest, among others. With 9 years of experience in university teaching, in chairs such as Botany, Plant Biology and Plant Biotechnology. Director and collaborator of research projects in different areas of the agricultural sector, biological control and molecular biology.



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    Abstract

    Ivonne Vaca Suquillo
    Politecnica Salesiana University, Ecuador

    LupinuspubescensBenth, also known by the name of Ashpachocho, is a native ornamental plant of Ecuador, due to the beauty of its flowers, it was declared as one of the species of patrimonial and emblematic flora of the Metropolitan District of Quito. The present work was developed in Quito - Ecuador. The current study was performed to evaluate three total nitrogen concentrations, coming from the Murashige and Skooog (MS) salts, components of culture media, during the tissue culture adaptation phase and, to determine the effect of the presence of BAP (Benzil amino purine) in the media, for the period of the multiplication. The seeds exposed to 4.88mMof the total nitrogen concentration presented the highest germination percentage among all the treatments (100%). Adding BAP (1ppm) to the medium, during multiplication phase achieved a higher average multiplication index (3.22). The results show that the reduction of total nitrogen in the culture medium was a determining factor in the increase of germination, besides that the presence of BAP in the culture medium favors the production of shoots per plant.

    Time:

    Title: Natural Herbal Remedies for the Treatment of Chronic Skin Ulcers in Animal Models

    Diana Sami
    The American University in Cairo, Egypt

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    Biography

    Diana Sami
    The American University in Cairo, Egypt

    Diana Sami is currently a graduate student in the Biotechnology program at the American University in Cairo (AUC). She earned her Bachelor degree in pharmaceutical Science from faculty of pharmacy,Helwan University, Egypt in 2011. She then obtained aDiploma in healthcare and hospital management from the American University in Cairo (AUC) in 2014. At present, Diana is interested in the development of cost effective treatment for skin ulcers using natural herbs.



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    Abstract

    Diana Sami
    The American University in Cairo, Egypt

    Chronic skin ulcers resulting from pressure ulcers or diabetes,affect nearly 2 million people each year and account for an annual healthcare costs of about $50 billion. Skin ulcers are commonly associated with elderly, bedridden, and debilitated patients, spinal cord injury and patients undergoing major orthopedic surgery. Diabetes increases the risk of skin ulcers because of its association with nerve damage (neuropathy),poor circulation and infection. Current treatment of skin ulcers includes traditional wound dressings, antibiotics, and debridement to remove necrotic tissues. One major disadvantage of using excessive antibiotics in skin ulcer treatment is antibiotics resistance. Herbal antimicrobials are expected to be non-cytotoxic, antibacterial, anti-inflammatory effect and will promote skin ulcer healing. Our aim in this study is to investigate various formulas of natural herbs e.g. Curcumin, and Ginsengs to enhance skin ulcer healing in animal models of skin ulcers. Our preliminary data show promising results in skin healing improvement both in Diabetic and Non diabetic pressure ulcer models. Ongoing work is verifying the healing and antibacterial potential of such formulas.

    Time:

    Title: Enhanced Production of L-glutaminase from Bacillus licheniformis by Taguchi DOE

    Hare Ram Singh
    BITS,India.

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    Biography

    Hare Ram Singh
    BITS,India.

    Dr. Hare Ram Singh having a long experience in the field of bioprocess engineering. He is actively engaged in the bioprocess development for the industrially important biomolecules using the microbial system. He has expertise in the process optimization, downstream processing and mathematical modelling of the bioprocess. By profession he is an academician cum researcher and presently serving as a Assistant Professor in the Birla Institute of Technology, Mesra, India.



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    Abstract

    Hare Ram Singh
    BITS,India.

    L-glutaminase (L-glutamine amidohydrolase, EC 3.5.1.2) is an important enzyme due to its property such as, it enhances the flavour of food and can also act as an anti-leukemic agent and as a biosensor. L-glutaminase is produced by micro-organisms like bacteria, fungi, yeast and including humans and animals. L-glutaminase hydrolyses glutamine to glutamic acid and ammonia. The objective of the present investigation is to qualitative and quantitative screening of potential L-glutaminase producers. The primary screening of L-glutaminase is performed by rapid plate assay method on the basis of pH dependent analysis. Bacillus licheniformis is observed as the maximum producer of L-glutaminase, which is then used for the further investigation. The secondary screening is performed under optimized conditions. Taguchi orthogonal method of optimization of six factors viz. Carbon source, Nitrogen source, Salts, Incubation period, pH and Temperature was used for the maximum production. The maximum enzyme production of L-glutaminase was observed at dextrose (2.5g/L), L-glutamine (0.9g/L), MgSO4.7H2O (0.4 g/L), NaCl (0.35 g/L), KH2PO4 (2.5 g/L), CaCl2.2H2O (0.9 g/L), Na2HPO4.2H2O (5 g/L), pH 7.0 at 370 C. An optimized enzyme production ensures high profitability and authentic significance in terms of its usage.

    Time:

    Title: Evaluation of Phenomenological Variables by Applying Compost and Digestate to Different Concentrations in Lettuce Cultivation (Lectuca Sativa L.)

    Jeisel Delgado Flores
    CIBA-IPN, Mexico

    Biography
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    Biography

    Jeisel Delgado Flores
    CIBA-IPN, Mexico

    Jeisel Delgado Flores is a master's student in the area of Applied Biotechnology at CIBA-IPN, Mexico. She is an engineer in food industries graduated from the InstitutoTecnológico del Altiplano (ITAT), Tlaxcala, México. She has experience in training people from rural communities, to process fruits and vegetables, dairy products and meat as an alternative to food preservation. She also has work as a researcher in conjunction with ITAT to find option to water treatment contaminated with heavy metals. Currently, his research work focuses on the development of a biofertilizer applicable to crops for human consumption.



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    Abstract

    Jeisel Delgado Flores
    CIBA-IPN, Mexico

    Increase in world population has caused an increasing demand for food, especially those of vegetable origin. Consequently, to meet this demand, farmers have used agrochemicals to improve crop yields. The objective of this work was to evaluate the growth of lettuce (Lactuca sativa L.) in response to the application of two types of organic fertilizers: compost and digestate, each of them obtained from organicwaste and cowdung; respectively. Plants with untreated soil were grown as a negative control, while plants grown in the presence of a chemicalfertilizer (NPK 17-17-17) were established as positive controls. In the case of plants treated with compost, concentrations of 20%, 40%, 50%, 60% and 100% were used; while for digestate they were 20%, 40%, 60% and 80% with frequencies of 15 and 30 days. The height of the plant, fresh weight, leaf area and root were measured. The experiments showed that the best compost treatment was the 100% application, in which the height of the plants was 52% higher and the fresh weights even times higher than the negative control. The Best digestate treatment was at a concentration of 80% and a watering frequency of 15 days. In the latter case, the height of the plants and fresh weightwas 65% and 89% greater tan the negative control. In summary, it is posible to improve the growth of lettuce by applying compost or digestate; however, more experiments are needed to find the right combination.

    Time:

    Title: Definition of Optimal In Vitro Conditions for Different Maturity Potato Cultivars

    Maia Kukhaleishvili
    Georgian Technical University, Georgia

    Biography
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    Biography

    Maia Kukhaleishvili
    Georgian Technical University, Georgia

    Maia Kukhaleishvili has completed his PhD at the age of 58 years from ST. Andrew the First Called Georgian University of the Patriarchate of Georgia. She is the director of Georgian Technical University, Biotechnology Center- Scientific-Research Center. She has published more than 10 papers in reputed journals and has a great experiences in the field of Agriculture and Biotechnology.



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    Abstract

    Maia Kukhaleishvili
    Georgian Technical University, Georgia

    The research aim was to determine optimal in vitro condition for propagation different maturity potato cultivars. Potato varieties were collected from in vitro potato collection of Georgian Technical University, Biotechnology Center according to their maturity: Early cultivars: Viviana, Red Sonia, Bellarosa, Vineta, Anushka; Medium early:Donata, Bernina, Madeira, Sante, Laura. Medium late: Brodie, Shepody,Jelly, Carola, Desiree. The influence of three types of combinations with temperature, humidity, light and photoperiod was studied on all three maturity potato cultivar’sin vitro development:I. T-23-250 C, H-80%, Lux-5-5500, 16h; II. T-25-270 C, H-75%, Lux-5-5500,16h: III. T-27-290 C, H-70%, Lux-5-5500, 16h;The results were evaluated after 17 days of reproduction. Best in vitro condition for all researched potato varieties was selected for their leave colors, rooting, and shoot formation. All potato cultivars morphological characterization was variable depending on the type of in vitro condition. It was revealed early maturity cultivars had maximum potential for in vitro propagation (5-6 nodes, average rooting 92% and shoot formation 94%) on combination: T-23-250 C, H-80%, Lux-5-5500, 16h. Combination: T-25-270 C, H-75%, Lux-5-5500,16h was best for medium early cultivars (5-6 nods, rooting 89% and shoot formation 90%). Theperfect in vitro reproduction of medium late varieties( 5-6 nodes, average rooting 87% and shoot formation 82%) was observed on the combination: T-27-290 C, H-70%, Lux-5-5500, 16h. As it is known, for in vitro developments of early medium and late medium potato cultivars are necessary 22-26 days, but our results were obtained in 17 days. Finally, optimal in vitro condition for propagation different maturity potato cultivars was defined.

    Time:

    Title: Demonstration of Antibacterial Activity in an Edible Mushroom of the Genus Pleurotus

    DIB Soulef
    University of Oran, Algeria

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    Biography

    DIB Soulef
    University of Oran, Algeria



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    Abstract

    DIB Soulef
    University of Oran, Algeria

    Pleurotus sp. (oyster mushroom) is a highly appreciated edible mushroom. Oyster mushrooms grow on tree trunks in forests. They can also be cultivated if we respect many parameters. Most superior mushrooms have nutritional value and interest in human health. According to the literature, about 700 species of these fungi possess interesting pharmaceutical properties and represent a considerable source of therapeutic compounds. In Algeria, there is little work on oyster mushrooms and in our knowledge few works on their antimicrobial activity has been reported, hence the interest for these mushrooms. The sporophores of oyster mushrooms are harvested in winter in the forest of Sig which is located at 52 km from Oran in western Algeria. The identification of fungi is carried out by classical morphological and microscopic methods. Crude extracts from dried fruit sporophores of oyster mushrooms are obtained by two techniques: hydrodistillation and Soxhlet. These extracts are tested in vitro, in Petri dishes, on the growth of 4 Gram + and Gram- bacterial strains (Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 6633, Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27453). The results obtained show a remarkable inhibitory effect of extracts of oyster mushrooms on the growth of the various microbial strains tested. The oyster mushrooms are therefore interesting candidates for obtaining bioactive antimicrobial extracts (substances) of therapeutic use.

    Time:

    Title: Utilization of Agro-Industrial Waste for Production of Protease Equipped with Industrially Desired Attributes

    Satbir Singh
    University of Jammu, India

    Biography
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    Biography

    Satbir Singh
    University of Jammu, India

    Satbir Singh has completed Ph.D. Biotechnology (2010-15) from Fermentation Biotechnology Lab, School of Biotechnology, University of Jammu J&K, India. His thesis title is Process optimization for production of thermo-alkalistable bacterial protease and its characterization. He has qualified Jawaharlal Nehru University combined entrance examination for Biotechnology (Masters programme) and availed research fellowship from Council of Scientific and Industrial Research during his doctoral research. He has published 15 articles including research papers, reviews and book chapters in reputed journals.



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    Abstract

    Satbir Singh
    University of Jammu, India

    Microbial proteases, due to their huge application potential, have attracted research attention and account for more than 60% of the world wide enzyme sale. However, large scale industrial application of proteases is hindered due to their poor performance under relatively hostile industrial conditions (extremes of temperature, pH), and high production cost of enzymes. The cost-effective production of a thermostable and wide range pH stable protease from a newly isolated Bacillus subtilis K-1 strain (BSK-1) was done using agro-industrial residues. Process optimization for protease production was conducted by one-variable-at-a-time and statistical approaches. The most significant variables for protease production were identified as incubation time, soybean meal, mustard cake and wheat bran. Optimization of these variables by central composite design of response surface methodology resulted in a substantial enhancement in protease yield. Purification of BSK-1 protease was done by chromatography and the purity was confirmed by zymogram and SDS-PAGE analysis. The BSK-1 protease was stable at wide pH and temperature range with optimum of pH 9.0 and 50C. Kinetic study of protease suggested high affinity for substrate and sequence analysis of encoding gene showed that K-1 protease is a serine alkaline protease. The enzyme was stable in presence of detergents and metal ions but strongly inhibited by PMSF. It is envisaged that the isolate BSK-1 could be a potential source of alkaline and thermostable protease for applications in industries like detergent, photographic and leather industry. Moreover, exploitation of agricultural wastes as substrates may pave the way for cost-effective production of industrially-suitable protease.

    Time:

    Title: Development of Process to Produce Biofuel from Lignocellulosic Biomass Using Natural Isolate

    Nidhi Adlakha
    JNU, India

    Biography
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    Biography

    Nidhi Adlakha
    JNU, India

    Nidhi Adlakha, working as Post Doctoral Fellow (Inspire Faculty) in JNU, New Delhi, India. She is working extensively towards understanding cellulose hydrolysis mechanism and developing system for efficient biofuel production. She obtained her PhD degree from International Centre for Genetic Engineering and Biotechnology, New Delhi, India. Her thesis work was on "Development of process to produce biofuel from lignocellulosic biomass using natural isolate" in which she had characterized and isolated potential cellulolytic enzyme systems from Paenibacillus polymyxa, isolated from the gut wood feeding insects. Till date, she have 10 papers published in reputed international journals such as Applied and Environmental Microbiology, Biotechnology for Biofuels and Scientific Reports. Alongwith this, she have 3 Indian patents.



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    Abstract

    Nidhi Adlakha
    JNU, India

    We report here the production of pure (R,R)- 2,3-butanediol (2,3-BDO) isomer by the non-pathogenic PaenibacilluspolymyxaICGEB2008 using lignocellulosichydrolysate as substrate. Experimental design based on Plackett-Burman resulted in identication of Mn and K as most crucial salt elements along with the yeast extract for 2,3-BDO production. Further experiments using Box- Behnken design indicated that both KCl and yeast extract together had major impact on 2,3-BDO production. Optimized medium resulted in 2,3-BDO production with 2.3- fold higher maximum volumetric productivity (2.01 g/L/h) and similar yield (0.33 g/g sugar) as compared to rich yeast extract-peptone-dextrose medium in the bioreactor studies. Considering that the balance substrate was channeled towards ethanol, carbon recovery was close to theoretical yield between the two solvents, i.e., 2,3-BDO and ethanol. Biomass hydrolysate and corn-steep liquor was used further to produce 2,3-BDO without impacting its yield. In addition, 2,3-BDO was also produced via simultaneous saccharication and fermentation, signifying robustness of the strain.

    Time:

    Title: Bioprospecting of Yeast Strains for Efficient Conversion of Lignocellulose to Bioethanol through Simultaneous Saccharification and Fermentation

    Ajay Kumar Pandey
    International Center for Genetic Engineering and Biotechnology, India.

    Biography
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    Biography

    Ajay Kumar Pandey
    International Center for Genetic Engineering and Biotechnology, India.



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    Abstract

    Ajay Kumar Pandey
    International Center for Genetic Engineering and Biotechnology, India.

    Lignocellulosic hydrolysate is a mixer of C6/C5 sugars and inhibitors (Furans, weak acids and phenolics) generated during the pretreatment.Therefore, robust yeast isolates with characteristics of C6/C5 fermentation and pretreatment inhibitor tolerance are pre-requisite forlignocellulosic ethanol production. Moreover, use of thermotolerant yeast isolates will reduce cooling cost, contaminations during fermentation, and also required for developing SSF and SSCF processes. Therefore, we evaluated the growth and fermentation performances of yeast isolates isolated from diverge natural habitats. The growth and fermentation performances were evaluated at 30oC and 40oC along with tolerance towards pretreatment inhibitors (Furfural, HMF, Acetic acid and Ethanol).K. marxianus NGY8 and O. thermophilaNGY11were able to grow onwide range of C6/C5 sugars including arabinose and cellobiose. S. cerevisiae NGY10 isolate, isolated from sugarcane bagasse distillery waste, produced maximum ethanol yield of 47.59 � 2.34 g/l and 46.8 � 3.11 g/l with the efficiency of 94.11% and 93.73% at 30oC and 40oC respectively,in 24 hours with glucose as a carbon source.This isolate produced ethanol yield of 8.17 � 0.14g/l with fermentation efficiency of 93.23% at 40oC, when rice straw enzymatic hydrolyzate was used as carbon source and displayed furfural (1.5 g/l), HMF (3.0 g/l), acetic acid (0.2% v/v) and ethanol (10.0% v/v) tolerant phenotypes. Together, NGY10 isolate could be potential yeast isolate for lignocellulosic ethanol production, C5 metabolic engineering and developing strategies for SSF and SSCF processes.

    Time:

    Title: Application of Bioles and Cutting Frequency in the Yield of Rye Grass

    Stefani Aletse Meza Zamora
    CIBA-IPN, Mexico

    Biography
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    Biography

    Stefani Aletse Meza Zamora
    CIBA-IPN, Mexico

    Stefani Aletse Meza Zamora is a biologist graduated from the Universidad Autónoma Metropolitana, Mexico; focused on the management of natural resources. She has studied marine ecology and is currently a student of the Master of Science in Applied Biotechnology at Centro de Investigacion en Biotecnologia Aplicada – IPN, developing and applying a bacterial biofertilizer to foliage crop.



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    Abstract

    Stefani Aletse Meza Zamora
    CIBA-IPN, Mexico

    In contrast to concentrated foods and high-grain diets, fodder provides an effective, economical and efficient way to feed animals intended for human consumption. Therefore, an increase in forage production is crucial since forages are an important part of the diet for ruminants. In this work, experiments were carried out using compost and digestate as substrate to evaluate grass yield. Seeds of Ballico grass (Lolium perenne) were sown in 1.5 kg pots containing a homogeneous mixture of soil and compost, followed by digestate addition at different concentrations (20, 40 and 60%). Moreover, three cutting frequencies (4, 5, and 6 weeks) and two digestate application frequencies (15 and 30 days) were also tested. Plants grown in pots without the addition of compost or digestate were used as negative controls, whereas plants grown in the presence of a chemical fertilizer were established as positive controls. Some phenological variables were analyzed. Results show that digestate has a positive and progressive effect on the yield of grass, which is reflected in measurements of Total Dry Weight (TDW) and Green Matter (GM). More importantly, digestate showed a higher grass yield than plants treated with a chemical fertilizer (NPK 17-17-17). For instance, the higher concentration of digestate (60%) exceeded the GM yield of negative controls by 220%. Altogether, these results show that the consecutive application of digestate (every 15 days) at a concentration of 60%, in combination with a defoliation frequency of 4 weeks have a positive effect on the yield of rye grass.

    Time:

    Title: Development and Characterization of Transgenic Pigeon Pea Plants Carrying Osruvb Gene Against Salinity Stress Tolerance

    Rakshita Singh
    CCSHAU,India.

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    Biography

    Rakshita Singh
    CCSHAU,India.



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    Abstract

    Rakshita Singh
    CCSHAU,India.

    Stress which arises due to environmental parameters such as salinity, drought, high temperature and cold disrupt the normal metabolism of plants. Almost all abiotic stress conditions generate osmotic stress in the plants. Salinity being a very vital problem for the survival of crops leads to major losses in crop productivity. Overexpression of DNA helicases like PDH 45, PDH 47 leads to abiotic stress tolerance (salinity tolerance in tobacco). However, the role of overexpression of RuvB, which is also a DNA helicase, in abiotic stress tolerance in plants has not been reported so far. Therefore, we have developed transgenic pigeonpea plants overexpressing OsRuvB gene, working under the control of CaMV35S promoter to analyse the effect of this gene on plants under saline conditions. The transgene integration in putative T0 plants has been confirmed through PCR analysis and transformation efficiency of 35-40% has been observed. The transgene integration has also been confirmed in T1 plants through PCR and these plants have been exposed to salinity stress. The physio-biochemical parameters such as relative water content, chlorophyll content, membrane stability test, proline content etc. have been studied to assess the tolerance level of the transgenic plants. The PCR positive transgenic plants are being analysed through southern hybridization and real time PCR to determine the transgene copy number.

    Time:

    Title: Direct Screening and Isolation of Microorganisms for a Biofertilizer Formulation

    Laura Jeannette Garcia Barrera
    CIBA-IPN, Mexico

    Biography
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    Biography

    Laura Jeannette Garcia Barrera
    CIBA-IPN, Mexico

    M. C. Laura Jeannette García Barrera is a Researcher of CIBA-IPN, Mexico. She studied Environmental Engineering at UPAEP, Mexico. She has a Master degree in Sciences in the specialty of Biotechnology from CINVESTAV, Mexico. Has experience evaluating the antifungal activity of plant extracts and works with molecular biology of viruses, bacteria and fungi. Has participated in projects in collaboration with the industry and is currently studying the use of biofertilizers and their impact on microbial communities in soils.



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    Abstract

    Laura Jeannette Garcia Barrera
    CIBA-IPN, Mexico

    Among genomic tools, metagenomics hasthepotential for characterizing large-scale population of bacterial genomes from environmental samples, without the need of isolation and growth. In this work, a metagenomic survey for bacteria present in three different substrates, namely soil, compost and digestate, were carried out. Bacteria belonging to genus with agricultural importance, such as plant growth promotion (PGP), were selected for further analysis. Briefly, bacteria with putative PGP activities were isolated and cultivated toward the goal of formulating a biofertilizer. Samples from soil, compost and digestate were culture in selective media (NBRIP, Pikovskaya Agar, Ashby Agar, NFB, R2A, FMA and Chitin Agar for the isolation of Lysobacter, Varioborax, Azospirillum, and Paenibacillus).A total of 152 strains were isolated, corresponding to 47, 42 and 63from soil, compost and digestate; respectively.Then, pure bacterial strainswere assessed as PGP agents by evaluating the production of indol aceticacid, gibberellic acid, and siderophore, as well as seed germination. Forty bacterial strains that showed PGP activity were tested for antagonistic behaviors. At last, 17strains were selected for PGP effects on Dactylisglomerata L.Strains with the higher PGP activities were identified as Microbacterium sp., Enterobacter ludwigii and Rahnella aquatilis. Taken together, the combination of metagenomic information and classical lab procedures allowed to formulate a novel biofertilizer for plant growth promotion, which is the first step to improve crop productivity.

    Time:

    Title: The Generation of G-Secretase Inhibitor-loaded PLGA-Fe3O4- Magnetic Nanoparticles

    Roa Bashmail
    Dublin City University, Ireland.

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    Biography

    Roa Bashmail
    Dublin City University, Ireland.



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    Abstract

    Roa Bashmail
    Dublin City University, Ireland.

    Cardiovascular disease is the number one killer in Ireland and the wider EU. A hallmark of the disease is the obstruction to blood flow due to the build-up of vascular smooth muscle (SMCs)-like cells within the vessel wall. Treatment options include percutaneous transluminal coronary angioplasty (PTCA) and the insertion of a stent a metal mesh tube into the obstructed vessel to keep it open. However, the vessel can become re-occluded due to the accumulation of SMC-like cells within stented area. The introduction of the 1 st generation drug-eluting stents (DES) has resulted in a paradigm shift for the treatment of in-stent restenosis with significant improvement in therapeutic outcomes. However, while polymer-coated DES have significantly reduced the incident of in-stent restenosis, current DESs lack the fundamental capacity for (i) adjustment of the drug dose and release kinetics and the (ii) ability to replenish the stent with a new drug on depletion. This limitation can be overcome by a strategy combining magnetic targeting via a uniform field-induced magnetization effect and a biocompatible magnetic nanoparticle (MNP) formulation designed for efficient entrapment and delivery of specific drugs that target the resident vascular stem cell source of the SMC. Magnetic nanoparticles (MNPs) containing magnetite (Fe 3 O 4 ) were fabricated, polymer coated with poly(DL-lactide- co-glycolide) polyvinyl alcohol [PLGA-PVA] and loaded with a -secretase inhibitor (GSI) of Notch signalling, DAPT using an oil in water emulsification technique. The free GSIs and GSI- loaded MNPs were assessed for drug release, the efficacy at controlling mesenchymal stem cell (MSC) growth (proliferation and apoptosis) and inhibiting myogenic differentiation under magnetic and non-magnetic conditions. The DAPT-loaded MNPs had an average hydrodynamic diameter of 351 d.nm Up to 40% of drug was released from MNPs within 48 h rising to 65% after 1 week under magnetic conditions. The Notch ligand, Jagged1 increased Hey1 mRNA levels and promoted myogenic differentiation of MSCs in vitro by increasing SMC differentiation markers, myosin heavy chain 11 (Myh11) and calponin1 (Cnn1) expression, respectively. This effect was significantly attenuated following treatment of cells with MNPs loaded with DAPT when compared to unloaded MNPs. Notch GSI loaded magnetic nanoparticles are functional at targeting vascular stem cells in vitro.

    Time:

    Title: Synergistic Effect of Cholesterol-vitamin E, Solubilized in Cyclodextrins, on Frozen-Thawed Bovine Semen

    Khellouf Allaeddine
    Abderrahmane-Mira-University,Algeria.

    Biography
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    Biography

    Khellouf Allaeddine
    Abderrahmane-Mira-University,Algeria.



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    Abstract

    Khellouf Allaeddine
    Abderrahmane-Mira-University,Algeria.

    Cryopreservation of sperm has been developed considerably in the recent years. However, during the different phases of the cryopreservation process spermatozoa are affected by various stresses. The aim of this study was to minimize this damage, particularly at the membrane level by supplementing the freezing extender medium with bioactive molecules, vitamin E and cholesterol. The two molecules are supposed to act in a complementary manner, cholesterol to reinforce the cell membrane and to fight against the cold shock, and vitamin E to fight against the oxidative stress. As the two molecules are lipophilic, they were both preloaded in cyclodextrins to enhance their solubility. The two molecules were used alone or in association. Sperm mobility (using CASA), sperm viability (using HOST) and levels of lipid peroxidation (using TBARs) were used to analyze sperm quality. The post-thawed results showed a significant protection of all sperm parameters when vitamin E and cholesterol were used simultaneously with 57.690.82 m/s, 39.876.3 %, 0.0460.12 nmol/108, for VCL, viability, and oxidative stress status, respectively. Associating cholesterol and vitamin E, both preloaded in cyclodextrins, seems to offer a real opportunity to improve bovine semen quality after freezing-thawing process.

    Time:

    Title: EhLINE1 and EhSINE1 Expression Profiling Using Next Generation Sequencing Reveals Uneven Transcript Distribution and Antisense Expression of Translationally-Silent Regions

    Devinder Kaur
    Jawaharlal Nehru University, India.

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    Biography

    Devinder Kaur
    Jawaharlal Nehru University, India.



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    Abstract

    Devinder Kaur
    Jawaharlal Nehru University, India.

    Entamoeba histolytica is a primitive parasitic protist. 11% of its genome is comprised of retrotransposons (EhLINEs and EhSINEs). LINE and SINE copies are generally maintained in a transcriptionally silent state with few copies being active. To determine the transcriptional status of E. histolytica retrotransposons, RNA-Seq was carried out in triplicate and reads corresponding to EhLINE1 and EhSINE1 were elucidated. Of total 948 copies of EhLINE1, and 493 copies of EhSINE1, 41 LINE1 and 129 SINE1 copies were shown to be transcribed. Of 41 expressed LINE1, 20 were full length while the rest had deletions/truncations. Uneven read distribution of ORF1 and ORF2 was observed with ratio of 1:40 respectively which was validated by northern with 1.5 kb bands from both ORF1 and 2 probes, ORF2 band is much brighter. Read-through transcription was observed for 26% LINE1 in both direct and antisense direction. Further, we looked for antisense expression of EhLINE1 and EhSINE1. Interestingly, 80% of EhLINE1 and 40% of EhSINE1 expressed copies showed antisense expression with LINE1 at a significantly high level compared with sense strand. Only the reverse transcriptase (RT) and Endonuclease (EN) region of LINE1 showed antisense expression whereas in SINE1 it was seen throughout the element. According to the current paradigm of LINE transcription, these elements are transcribed into a single polycistronic transcript from a 5end internal promoter. Our data suggest a complex transcriptional profile whereby transcripts corresponding to different regions of EhLINE1 accumulate to different levels, and antisense transcripts presumably attenuate translation of some of the transcripts.

    Time:

    Title: Screening, Characterization, and Biocatalytic Capacity of Lipase Producing Wild Yeasts from Makiling Forest Reserve (MFR) Philippines

    Julianne Vilela
    University of the Philippines, Philippines

    Biography
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    Biography

    Julianne Vilela
    University of the Philippines, Philippines

    Ms. Julianne Vilela is a University Researcher and Bioinformatician at the Philippine Genome Center, University of the Philippines. Ms. Vilela extensive training includes: Genome-wide Association Studies (GWAS), Quantitative Genetics and Genomics at Iowa State University, Iowa, USA, and Plant Genome and Bioinformatics Training at Boyce Thompson Institute for Plant Sciences, Cornell University, USA.



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    Abstract

    Julianne Vilela
    University of the Philippines, Philippines

    Lipases (triacylglycerol acylhydrolases, E.C. 3.1.1.3) are enzymes generally used in several industrial applications. However, even with the numerous industrial applications of lipases, there are limited studies aiming to characterize and optimize lipase activity especially that of yeast lipase-producing microorganisms. This work aims to select and identify lipase-producing yeasts isolated from Makiling Forest Reserve (MFR), Philippines and to optimize conditions to maximize lipase production. A total of 144 wild yeasts were tested for their lipase producing potential and strain B1-7 showed the highest lipase activity in both solid and liquid selection media (7.6 EAI and 0.082 U/mL-min activity, respectively). Strain B1-7 was molecularly identified by sequencing the ITS1-5.8S-ITS2 region of the fungal rRNA operon DNA as Cryptococcus flavescens.The optimum conditions for maximum lipase activity (0.66 U/mL-min) of the putative C. flavescens strain obtained using Response Surface Method (Box-Behnken Design) were 5.0 C:N value, pH 6.0 and 0.5% inducer. Lipase activity was significantly affected by the C:N to percent inducer interaction (p= 0.010) and % inducer (p = 0.040). After a 72h fed-batch fermentation experiment, lipase activity was 0.115 U/mL-min. A negative correlation (R2= -0.426) was observed between lipase activity and biomass suggesting that lipase production did not depend on biomass. Moreover, no change in lactose concentration was observed suggesting that it was not used as the primary source of carbon. Hence, we can exploit the potential of producing a new strain for industrial application.

    Time:

    Title: Study of Preparation Humate Gel AG Biological Activity

    Lina Pakalniskyte
    Institute of Botany of Nature Research Centre, Lithuania.

    Biography
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    Biography

    Lina Pakalniskyte
    Institute of Botany of Nature Research Centre, Lithuania.

    Lina Pakalniskyte, junior researcher, research fellow of Nature Research Centre of Institute of Botany of Plant Physiology Laboratory (of 1997). She started PhD studies in 2016. The topic of the dissertation is Research of plant physiological responses to enzymatic compositions of probiotics. The objective of work: to investigate the effects of fermented probiotics on agricultural crop growth, development, yield quality and soil structure formation.



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    Abstract

    Lina Pakalniskyte
    Institute of Botany of Nature Research Centre, Lithuania.

    Aiming to optimize and improve the state of the art technologies for the production of winter crops, which would ensure high and stable crop yield under the Lithuanian climatic conditions, it is important to use a modern agrotechnique and agrochemistry.Hormonal system, along with other levels of regulation, determine the character of physiological and biochemical processes, biomass accumulation in the plant, but the possibility of phytohormone application for increasing crop yields did not establish in plant tissues hormones metabolize, are destroyed or combined (Merkys, Novickienė, 2000). Meanwhile, in plants treated with fertilisersthe corresponding changes of phytohormones status occur, synthesis of individual hormones and rearrangement in plant organs change, organogenesis and growth processes alter.Purpose of the work was to perform studies on biological activity of preparation Humate Gel AG,under controlled laboratory conditions comparing with the action of phytohormones IAA, GR, CK and to present the assessment of Humate Gel AG activity.Commercial preparation Humate Gel AG HydroThermoDynamic Technology, Ukraine was used. The microbiological content of this composition is: Azotobacter, R.hizobium, Bradyrhizobium, Bacillus subtilis, B. cereus, B. megaterium, Lactobacillus, Trichoderma..PreparationHumate Gel AG was applied as aqueous solution at the rate of 70 ml/ha, 350 ml/ha, 700 ml/ha, 1 l/ha and 2 l/ha.Evaluation of biological activity of Humate Gel AG was performed comparing its performance with phytohormones: 3-indolylacetic acid (IAA), gibberellic acid (GA), cytokinin (CK) performance upon growth and development of individual plant organs and their parts. Application of biological test methods with seedlings, individual plant organs and their parts for assessment of biological activity of HumateGel AG under laboratory conditions revealed that optimal concentrationsof 700 ml/ha promote cell stretch growth, rooting and stem internodal growth, and thus was characterized by action typical to phytohormones auxin, gibberellin and partially cytokinin. Summarizing the data, we can say that the preparationHumate Gel AG is characterized by auxin and partially gibberellin and cytokinin activity it promoted cell division (IAA), stretch growth (GA), root formation (IAA), stem stretch growth (GA and IAA) and seed germination (CK).

    Time:

    Title: Bioengineering of Yeast Cell for Biodiesel Production

    Priya Kumari
    DBT-ICGEB, India

    Biography
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    Biography

    Priya Kumari
    DBT-ICGEB, India



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    Abstract

    Priya Kumari
    DBT-ICGEB, India

    Due to increased oil demand, depleting fossil fuels and greenhouse gas emissions,biofuels production are getting much attention. The fatty acid based biofuels (fatty acids ethyl ester/biodiesel, fatty alcohol, etc.) produced from microbial cells have emerged as ideal alternatives to fossil oils, with significantpluses over plant, animal and algae oils.Saccharomyces cerevisiae is a most studied industrial model microorganism and also its fatty acid production abilityhas been increased by metabolic engineering approach. But still the cost of the process limits its industrial production therefore, more research is required. Here, we are addressing this issue bysequential metabolic engineering approach. In order to synthesize biodiesel in yeast cells, we integrated wax ester synthase (WS2) gene fromMarinobacterhydrocarbonoclasticusinto its genome. The genetic engineering approaches have focused on high-level biodiesel production by rewiring metabolism pathways to upsurge carbon flux towards fatty acid CoA synthesis, by increasing the cofactor supply, and disrupting the degradation pathway.

    Time:

    Title: Appraisal of Microcarrier Suspension in a Single-Use Shaken Bioreactor with Conical Bottom

    Gregorio Rodriguez
    University College London, UK.

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    Biography

    Gregorio Rodriguez
    University College London, UK.



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    Abstract

    Gregorio Rodriguez
    University College London, UK.

    The pharmaceutical industry is at the forefront of the production of drug products using mammalian cell-based cultures, some of which rely on anchorage dependant cells, with single-use technology bioreactors gaining prominence. Whilst often adapted to suspensioncultures, hybridoma and other cells give increased product yields when culturedattached to a substrate, such as microcarriers, resulting in smaller fermentation runs at higher densities. Microcarriers are also used in tissue engineering andcell for therapy culture. Stem cells are adherent-dependantcells and traditional 2-dimensional static cultures rely on disposable multilayervessels, which have become the common route for stem cell expansion. It has been a recent industry trend to use shaken bioreactors at large scale in the upstream process, performing mammalian cell culture in single-use bags which eliminate the need for cleaning in place, offer flexibility and lower production down-times. Whilst currently still to be fully developed, large scale production of stem cells would require the use of 3-dimensional dynamic culture methods by employing microcarrier technology, asdemonstrated by Frauenschuh et al. (2007). Microcarriers consist of sphericalbeads with a size of 100-300 μm, and can be made of a wide variety of materials, amongst others: plastics, glass, acrylamide, silica, silicone rubber, cellulosedextran and collagen. Medium to large scale single-use devices would greatly benefit the consistency of stem cell culture whilst maintaining potency. Whilst most studies have focused on investigating optimal compositions of microcarrier materials, their concentration and cell culture media, little researchhas been undertaken on the engineering aspects of microcarrier use.The aim of the work is to characterize the suspension dynamics of microcarriers in a cylindrical orbitally shaken bioreactor (OSR) with conical bottoms of different heights. This study builds upon previous works of the research group (1-4) for a flat bottom reactor, where increases in Froude number were found to determine a mean flow transition which was found to be instrumental in determining the just-suspended speed. The dynamicsof solid suspension is studied using commercially available Cytodex-3,stained with trypan for improved visual contrast and image acquisition. The experimental procedure allows to estimate not only the speed required for the solids to lift from the vessel bottom, but also the conditions at which a homogenous distribution of microcarreirs is obtained.Preliminary results indicate that the presence of the conical bottom improves solid suspensionby requiring lower agitation rates for the microcarriers to lift fromthe bottom completely. The critical Froude, which determines theflow type controlling the bioreactor, can be used to scale thesuspension of microcarriers in OSRs.

    Time:

    Title: Microsatellite Polymorphism in Some Mismatch Repair (MMR) and Tumor Suppressor (TS) Genes Shows Significant Association with Gastric Cancer

    Renu Verma
    Guru Gobind Singh Indraprastha University, India

    Biography
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    Biography

    Renu Verma
    Guru Gobind Singh Indraprastha University, India

    Renu Verma is pursuing her Ph.D.in Biotechnology at University School of Biotechnology, Guru Gobind Singh Indraprastha University, New Delhi. She is investigatingassociation of microsatellite instability (MSI) inselected mismatch repair and tumor suppressor genes withgastric cancer. She is also involved in RNA-Seqbased transcriptome profiling of gastric tumors to identify differentially expressed genes and other genetic factors involved during gastric tumorigenesis.



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    Abstract

    Renu Verma
    Guru Gobind Singh Indraprastha University, India

    Genetic and epigenetic changes in different mismatch repair genes and tumor suppressor genescontribute to molecular pathogenesis of some cancers including gastric cancer (GC), the fifth most prevalent cancer causing third most cancer-related mortality in the world. Early diagnosis of GC is difficult as most of the symptoms arisein advanced stages. Microsatellites,also known as Simple Sequence Repeats (SSRs),representshort iterations of 1-6 nucleotide long units, non-randomly distributed in coding and noncoding regions of almost all genomes. Polymorphismat microsatellite regions, commonly referred to asMicrosatellite Instability (MSI), has been clearly implicated in colorectal cancer andnowalso being explored ingastric and other cancers.We investigatedMSI attwenty five microsatellite loci present inthe coding regions of seven mismatch repair genes(PMS1, PMS2, MLH1, MLH3, MSH2, MSH3 and MSH6) and five tumor suppressor genes (TGFBR2, PDCD4, DLC1, EGR1 and CHD5). RNA was isolated from cancerous and adjacent non malignant tissue (5cm apart from tumor) at GB Pant Hospital, New Delhi. The PCR amplicons were sequenced to detect length and sequence polymorphism at selectedmicrosatellite loci. Ourresults showpolymorphismat microsatellite loci in MSH3,MSH6, MLH3,DLC, PDCD4, and TGFBR2 genes in tumor RNA. Expression of genes showing microsatellite mutations analyzed usingreal time PCR showed differential expression in tumor versus control tissues.Our findings encourage further exploration of the role of MSI in molecular pathogenesis of GCto facilitate development ofdiagnostic molecular biomarkers and drug targets for better management of gastric cancer.

    Time:

    Title: The Effect of Stress on Behavior and Immunity in Wistar Rats

    Sansri Soraya
    University Badji Mokhtar Annaba, Algeria.

    Biography
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    Biography

    Sansri Soraya
    University Badji Mokhtar Annaba, Algeria.



    Abstract
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    Abstract

    Sansri Soraya
    University Badji Mokhtar Annaba, Algeria.

    Stress is a major current problem both in humans and in animals and implement strategies to limit sometimes adverse effects. In addition, exposure to stress causes behavioral and immune disorder in rodents. Experimentally, this modification is based on the intensity and type of exerted stress. The objective of this work is to study the effect of three types of stresses, acute restraint, and predation by separation to assess immune and behavioral changes in the Wistar rat. Comparison between the three types of stresses, Behavioral and adaptive changes in the rat are an attempt to identify the behavioral parameters evaluated from the open fields andmaze. Our results on stressed rats showed the following: Increased anxiety with onset of depression evaluated in tests in an elevated cross maze and open field. Impairment of spatial memory. Disturbance of the immune system cells and humoral response (monocytes and lymphocytes).

    Time:

    Title: Biological Desulfuration of Industrial Effluents: Isolation of Sulfato-Reducer Microorganisms

    Asmaa Fathi

    Biography
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    Biography

    Asmaa Fathi



    Abstract
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    Abstract

    Asmaa Fathi

    BSRs can be isolated from a variety of environmental sources in terms of temperature, salinity, atmospheric pressure, pH, and osmotic conditions. They survive for several days in an aerobic environment. They are frequently isolated from soils, fresh and marine waters. The high sulphate content of marine and polluted waters explains their high prevalence and their multiplication in these ecosystems. The conditions necessary for their development are usually the presence of sulphates, organic matter and the existence of a low redox potential. Urban and industrial wastewater loaded with organic matter can induce the formation of unpleasant odors following a biological fermentation. In this study, we investigated the impact of the nature of the effluent and its composition on the amount of sulfur present in the pipelines. The collected data are analyzed, in correlation with the industrial activity of the zone and the nature of the rejection. These results are supplemented by other samples in order to determine the microbiological quality of the effluent. Moreover, the presence of sulphate-reducing microorganisms (main route of transformation of sulphates under the conditions of anaerobic storage of effluents) is verified.

    Time:

    Title: 2,3-Diaryl indenone and 2-Chloro-3-Amino indenone Derivatives as Selective Inhibitor of DNA Repair Enzyme AlkB and AlkBH3

    Richa Nigam
    IITH, India.

    Biography
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    Biography

    Richa Nigam
    IITH, India.



    Abstract
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    Abstract

    Richa Nigam
    IITH, India.

    Human AlkB homologue-3 (AlkBH3) is a DNA repair enzyme that demethylases N1-methyladenine and N3-methylcytosine base lesions. AlkBH3 is an ortholog of bacterial AlkB and also known as prostate cancer antigen-1 (PCA-1) and known to express abundantly in several types of cancers, including prostate cancer. Because of its immense biological and clinical significance, extensive efforts are being directed in developing selective inhibitors for AlkBH3. Here we report synthesis, screening and evaluation of panel of arylated indenone and 2-chloro-3-amino indenone derivatives as new class of specific inhibitors of AlkB family of DNA repair enzymes. An efficient synthesis of 2,3-diaryl indenones from 2,3-dibromo indenones was achieved via Suzuki-Miyaura cross-coupling. Further, synthesis of 2-chloro-3-amino indenone derivatives was achieved from 2,3-dichloro indenones via addition elimination method. Using a robust quantitative assay, we have obtained few inhibitors that showed a unique competitive inhibition mechanism against DNA substrate and a mixed inhibition against 2OG co-substrate. These AlkBH3 inhibitor rendered human cells hypersensitive to exposure to DNA/RNA damaging alkylating agent. This discovery is the first report of an indenone derivative as inhibitors targeting bacterial and human DNA alkylation repair and provides a framework from which second-generation indenone derivatives may be developed.

    Time:

    Title: Identification of Bread Wheat Seed-Specific bZIP Transcription Factors Binding Sites by Genome-Wide Invitro Binding Analysis

    koushik Shah
    National Agri - Food Biotechnology Institute, India.

    Biography
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    Biography

    koushik Shah
    National Agri - Food Biotechnology Institute, India.



    Abstract
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    Abstract

    koushik Shah
    National Agri - Food Biotechnology Institute, India.

    Basic leucine zipper (bZIPs) are dimeric sequence-specific transcription factors (TFs) that are unique to eukaryotes and play an important role in various biological processes including seed development and maturation, plant immunity and defense, and biotic and abiotic stress. In humans and other vertebrates bZIP TFs DNA binding sites gene promoters are well characterized but not much is known in plants. Problem is more pronounced in economically important crop like wheat. Recently an ordered draft sequence of hexaploid wheat (Triticum aestivum) genome and transcriptome analysis has opened new opportunities to study the roles of TFs proteins in gene regulation in wheat. Particularly, we are interested in analyzing the structure and functions of bZIP TFs of wheat. In last few years there has been a spurt in breakthrough technologies that identify TF-binding sites in whole genome (e.g. PBM, ChIP-seq, Bind-n-seq/DAP-seq). The new protocols are helping in identify hitherto unknown binding sites of TFs both in vivo and in vitro. We have cloned seed- specific bZIPs and adapted high-throughput bind-n-seq methodology to identify their genome- wide binding sites. Bind-n-Seq is a simple and robust method in which bZIPs are incubated with bar coded random oligonucleotides libraries (70 mer) with random binding sequences. Pure bZIP bound oligonucleotides are isolated and are sequenced using illumina platform. Based on abundance binding sites are scored. One of significant advantage of bind-n-seq over other method is that large numbers of binding sites are captured for each bZIP that is used in constructing transcription factor binding landscape.

    Time:

    Title: Design of Plastic Materials Based on Non-Stick Polypropylene for Improve Food Packaging Hygiene

    Maria Mekouar

    Biography
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    Biography

    Maria Mekouar



    Abstract
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    Abstract

    Maria Mekouar

    Before being consumed, food comes into contact with many materials namely plastic materials during the various processes of production, processing, storage, preparation and serving of food. This contacting is concerned with the phenomena of physical and chemical interaction, the bonding and also the transfer of material. This can lead to adverse effects both in terms of quality, hygiene and public health. The aim of this work is oriented towards the design of anti-adhesion polypropylene materials by modifying in the standard formula of the basic material. In this work, three different formulations incorporating known natural components of their antibacterial effect were studied. The material was developed by extrusion process. The anti-biofilm effect of the material was confirmed by adhesion tests and observations by MEB scanning electron microscopy. The new material was analyzed by infrared in order to highlight the structural modifications of the groups exposed on the surface of pipelines. The contact angle allows the detection of any modifications of the hydrophobicity and the acid-base character which will make it possible to explain certain variabilities observed after adhesion test. The mechanical properties of the new material were also evaluated and discussed and compared to the properties of the material developed according to the standard formula.

    Time:

    Title: Production and Characterisation of Extracellular Chitinase from a Novel Isolate Chitinophaga sp. S167

    Sonia Sharma
    Punjab University,India.

    Biography
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    Biography

    Sonia Sharma
    Punjab University,India.



    Abstract
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    Abstract

    Sonia Sharma
    Punjab University,India.

    Chitinases cleave β-1,4 N acetyl glucosamine linkages and thus have the ability to degrade chitin in cell wall of fungi and exoskeleton of insects. They are potential antifungal, insecticidal and nematacidal agents. Chitinases have been isolated from bacteria, fungi, plants, insects, crustaceans, animals and humans. Based on the site of action on the substrate, they are classified as exochitinases or endochitinases. An isolate S167 (GeneBank Acc. No. KP017541) showing 98.62%, 98.47 and 97.78% 16S rDNA similarity to Chitinophaga ginsengisoli, C. filiformis and C. pinensis respectively, on EzTaxon was isolated from soil rich in organic decaying matter. Chitinophaga sp. S167 shows chitinolytic activity and produces extracellular chitinase. The chitinase from Chitonophaga sp. S167 inhibited Cladosporium sp., Alternaria sp. and Fusarium sp. The enzyme was found to be optimally active at 40⁰C and pH 6. The chitinase was maximally induced at 72 hours by 1.5% swollen chitin when incubated in the medium of pH 8 at 35C. The enzyme was purified using ion exchange and hydrophobic interaction chromatography. The purity of the enzyme was checked by SDS-PAGE which showed a band with an apparent molecular weight of 50 kDa and its activity was confirmed by zymography. Further studies are being carried out to characterize the enzyme and determine its substrate specificity.

    Time:

    Title: Study of the Invitro Germination of Immature Embryos of Orange Trees (Citrus sinensis)

    Karim Mahmoudi
    Ibn Tofail University,Morocco.

    Biography
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    Biography

    Karim Mahmoudi
    Ibn Tofail University,Morocco.



    Abstract
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    Abstract

    Karim Mahmoudi
    Ibn Tofail University,Morocco.

    Oranges constitute the major part of the production of citrus fruit, which is the most important fruit group in international trade.The creation of new triploid hybrids via the rescue of immature embryos allows diversification of the varietal profile of orange trees. The objective of this study is to optimize the in vitro germination of immature embryos according to the chemical composition of four in vitro culture media in two varieties of orange trees (Pineapple and Parson Brown). At the maturity stage, the fruit was harvested and the extracted seeds were classified according to their size. Only small or flat seeds were cultured in a base medium of Murashig and Tuker (MT) under sterile conditions. The different growth regulator concentrations were tested to obtain the best medium for seedling development: M1 (MT + 1 mg / l GA3), M2 (MT + 1mg / l Kenitin + 0.5 mg / l BAP + 0.1 mg / l ANA), M3 (MT + 25 mg / l adenine sulphate), M4 (MT + 0.5 mg / l Kenitin + 0.5 mg / l BAP + 1 mg / l GA3). For both Orange varieties Pineapple and Parson Brown, the germination rate is maximum in M3 medium respectively at percentages of 100% and 90%. varieties between 6 and 7 days. With respect to growth rate (mm / week), both varieties knew a variation in the four media. Similarly, the maximum acclimation rate in the M1 medium is 80% and 90% respectively for the Pineapple and Parson Brown varieties. In general, the smaller the embryos, the more sensitive they are to the composition of the culture medium. It is therefore essential to optimize the components of the medium to promote their growth and their in vitro developments. Therefore, the medium M1 (MT + 1 mg / l GA3) remains the best to promote good germination in short time and a better acclimatizationrate.

    Time:

    Title: High Variability of Genetic Pattern in Giant Clam ( Tridacna Maxima) Populations within Red Sea Coastal

    Abdelwaheb Ben Othmane
    University of Monastir, Tunisia

    Biography
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    Biography

    Abdelwaheb Ben Othmane
    University of Monastir, Tunisia



    Abstract
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    Abstract

    Abdelwaheb Ben Othmane
    University of Monastir, Tunisia

    Information on the genetic population structure of endangered giant clams is important for conservationprograms and the understanding of ecological and evolutionary processes. In this study, thegenetic population structures of five populations of the giant clams, Tridacna maxima, from five coastal localities of the red sea in Saudi arabiawere screened for a fragment of the cytochrome c oxidase I gene. Forty-four clam were collected from 5 localities. Analyses of sequence variation in a 685 bp segment of the mitochondrial cytochrome oxidase c subunit I(cox1) gene revealed low nucleotide diversity but high genetic diversity within populations (Hd D 0.84). Anoticeable genetic differentiation was found in all populations. However, genetic differentiation was not correlated with geographical separation. eighteen haplotypes were identified. Connectivity between populations, was relatively high, which could be explained by the high dispersal ability of thelarval stage.

    Time:

    Title: Modification of filC to Increase Protein Solubility by Using a Bioinformatic Study

    Masoumeh Bagheri
    Razi vaccine and serum research institute, Iran

    Biography
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    Biography

    Masoumeh Bagheri
    Razi vaccine and serum research institute, Iran



    Abstract
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    Abstract

    Masoumeh Bagheri
    Razi vaccine and serum research institute, Iran

    Flagellin as a virulent factor of pathogenic gram negative bacteria, is responsible for several functions such as movement, adhesion and invasion. It is the major structural protein of bacterial flagella which is the ligand of TLR5. Also, it has a potency to be an adjuvant. Since, protein engineering by amino acid substitution and deletion can be a logical way for making custom built protein derivatives of flagellin for specific applications, we performed a bioinformatic study to find the best model for gene manipulation for flagellin. Gene modification was carried out by side-directed mutagenesis. Consequently, modified gene, truncated modified fliC [tmFliC],was cloned and expressed in pET-21 vector and then expressed proteinwas purified to determine its property. Results showed a high purification efficacy comparison with other reported. This finding resulted fromreduction of the hydrophobicity in C-terminus which may express a more soluble flFliCprotein.

    Time:

    Title: Successor of Dairy Milk

    Dhruv Thanki
    V V P Engineering College, India

    Biography
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    Biography

    Dhruv Thanki
    V V P Engineering College, India



    Abstract
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    Abstract

    Dhruv Thanki
    V V P Engineering College, India

    Three plain soyamilk, coconut milk, rice milk (full-fat and light) replaced 100% of the whole dairy (cow) milk in control formulas for muffins, popovers, and frozen custard-based ice milk dessert. Milks incorporated differed in total solids, pH, viscosity, particle size distribution, and colour when assessed instrumentally. Quality of the prepared food products was evaluated with objective techniques, and consumer sensory panellists assessed appearance, flavour, texture, and overall acceptability. In popovers, neither volume nor acceptability differed with soy milk substitution. For muffins, flavour, texture, and overall acceptability did not differ, and appearance of the soy milk products was preferred,either soy milk can successfully be substituted for dairy milk. Soya milk is one of plant-based complete proteins and cow milk is a rich source of nutrients as well. Cow milk is containing near twice as much fat as and ten times more fatty acids do soymilk. soymilk contains more Fe (ten time more) than does cow milk. Cow milk and soy milk contain nearly identical amounts of protein and water and fibre is a big plus, dairy has none. Although what we choose to drink is really a matter of personal preference and our health objectives but looking at the comparison, soy looks like healthier choice.

    Time:

    Title: Enzyme Stabilization by Immobilization for an Industrial Uses

    Hadjer Zaak
    IbnKhalboun University, Algeria

    Biography
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    Biography

    Hadjer Zaak
    IbnKhalboun University, Algeria



    Abstract
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    Abstract

    Hadjer Zaak
    IbnKhalboun University, Algeria

    Lipases are among the industrial enzymes most used because of their high activity and stability as well as the wide range of substrates they can catalyze. Their use requires immobilization to allow their reuse and easy separation of the reaction mixture. This technique may also improve other enzymatic properties such as stability or activity and selectivity, etc. Stability is a key factor that can limit the use of all enzymes. In the present study, phospholipase Lecitase Ultra (LU) and Thermomyceslanuginosus lipase (TLL) were immobilized in octyl-agarose (OC) beads. The aim was to find a strategy to improve the stability of immobilized enzymes and Limiting their desorption in the reaction medium.

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